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West nile virus antibody prevalence in horses of Ukraine.

Ziegler U, Skrypnyk A, Keller M, Staubach C, Bezymennyi M, Damiani AM, Osterrieder N, Groschup MH - Viruses (2013)

Bottom Line: West Nile virus (WNV) is a mosquito-borne virus of global importance.Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world.Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies.

View Article: PubMed Central - PubMed

Affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, D-17493 Greifswald-Insel Riems, Germany. martin.groschup@fli.bund.de.

ABSTRACT
West Nile virus (WNV) is a mosquito-borne virus of global importance. Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world. In Ukraine, WNV infections in humans and birds were first reported more than 25 years ago, yet the current epidemiological status is quite unclear. In this study, serum samples from over 300 equines were collected and screened in order to detect current WNV activity in Ukraine with the goal to estimate the risk of infection for humans and horses. Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies. The results clearly revealed that WNV circulates in most of the regions from which samples were obtained, shown by a WNV seroprevalence rate of 13.5% of examined horses. This is the first topical report indicating the presence of WNV infections in horses in Ukraine, and the results of this study provide evidence of a widespread WNV circulation in this country.

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Competitive ELISA (S/N%) results of all 295 investigated horse sera. (1 = reactive samples, 2 = negative samples)
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viruses-05-02469-f002: Competitive ELISA (S/N%) results of all 295 investigated horse sera. (1 = reactive samples, 2 = negative samples)

Mentions: From the 310 sera, 295 samples were investigated using the competitive WNV ELISA. Volumes of the remaining 15 samples were so low (<50 µl) that they were only used for different NT (Table 1). The ELISA results showed a high rate of seropositive horses in different regions of Ukraine (Table 2). A total of 49 out of the 295 sera were reactive (Figure 2). Resulting in an overall ELISA prevalence rate of 16.6%., WNV antibody-positive animals were discovered in seven of the 14 investigated Ukrainian regions and high ELISA prevalence rates between 20% and 25% were determined for the Luhanska, Kharkivska and Kirovohradska regions. Even higher prevalence rates were obtained for Poltavska (33%) and Zaporizka (83%), with the caveat, however, that available sample numbers were small (n=6) (Table 2). All 49 ELISA-positive samples were investigated further by the different NT to exactly determine the virus resulting in ELISA seropositivity.


West nile virus antibody prevalence in horses of Ukraine.

Ziegler U, Skrypnyk A, Keller M, Staubach C, Bezymennyi M, Damiani AM, Osterrieder N, Groschup MH - Viruses (2013)

Competitive ELISA (S/N%) results of all 295 investigated horse sera. (1 = reactive samples, 2 = negative samples)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814598&req=5

viruses-05-02469-f002: Competitive ELISA (S/N%) results of all 295 investigated horse sera. (1 = reactive samples, 2 = negative samples)
Mentions: From the 310 sera, 295 samples were investigated using the competitive WNV ELISA. Volumes of the remaining 15 samples were so low (<50 µl) that they were only used for different NT (Table 1). The ELISA results showed a high rate of seropositive horses in different regions of Ukraine (Table 2). A total of 49 out of the 295 sera were reactive (Figure 2). Resulting in an overall ELISA prevalence rate of 16.6%., WNV antibody-positive animals were discovered in seven of the 14 investigated Ukrainian regions and high ELISA prevalence rates between 20% and 25% were determined for the Luhanska, Kharkivska and Kirovohradska regions. Even higher prevalence rates were obtained for Poltavska (33%) and Zaporizka (83%), with the caveat, however, that available sample numbers were small (n=6) (Table 2). All 49 ELISA-positive samples were investigated further by the different NT to exactly determine the virus resulting in ELISA seropositivity.

Bottom Line: West Nile virus (WNV) is a mosquito-borne virus of global importance.Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world.Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies.

View Article: PubMed Central - PubMed

Affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, D-17493 Greifswald-Insel Riems, Germany. martin.groschup@fli.bund.de.

ABSTRACT
West Nile virus (WNV) is a mosquito-borne virus of global importance. Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world. In Ukraine, WNV infections in humans and birds were first reported more than 25 years ago, yet the current epidemiological status is quite unclear. In this study, serum samples from over 300 equines were collected and screened in order to detect current WNV activity in Ukraine with the goal to estimate the risk of infection for humans and horses. Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies. The results clearly revealed that WNV circulates in most of the regions from which samples were obtained, shown by a WNV seroprevalence rate of 13.5% of examined horses. This is the first topical report indicating the presence of WNV infections in horses in Ukraine, and the results of this study provide evidence of a widespread WNV circulation in this country.

Show MeSH
Related in: MedlinePlus