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West nile virus antibody prevalence in horses of Ukraine.

Ziegler U, Skrypnyk A, Keller M, Staubach C, Bezymennyi M, Damiani AM, Osterrieder N, Groschup MH - Viruses (2013)

Bottom Line: West Nile virus (WNV) is a mosquito-borne virus of global importance.Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world.Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies.

View Article: PubMed Central - PubMed

Affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, D-17493 Greifswald-Insel Riems, Germany. martin.groschup@fli.bund.de.

ABSTRACT
West Nile virus (WNV) is a mosquito-borne virus of global importance. Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world. In Ukraine, WNV infections in humans and birds were first reported more than 25 years ago, yet the current epidemiological status is quite unclear. In this study, serum samples from over 300 equines were collected and screened in order to detect current WNV activity in Ukraine with the goal to estimate the risk of infection for humans and horses. Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies. The results clearly revealed that WNV circulates in most of the regions from which samples were obtained, shown by a WNV seroprevalence rate of 13.5% of examined horses. This is the first topical report indicating the presence of WNV infections in horses in Ukraine, and the results of this study provide evidence of a widespread WNV circulation in this country.

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Sampling sites of the horse sera with focus on known West Nile virus (WNV) risk areas.
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viruses-05-02469-f001: Sampling sites of the horse sera with focus on known West Nile virus (WNV) risk areas.

Mentions: Horse sera from 310 animals kept in 14 different Ukrainian regions were collected in 2010 and 2011 (Figure 1, Table 1). Sera were screened by a commercial WNV competition ELISA, which essentially detects flavivirus antibodies. Therefore ELISA-positive results were verified by virus neutralization tests (NT) to identify WNV or cross-reactivity specific reactions.


West nile virus antibody prevalence in horses of Ukraine.

Ziegler U, Skrypnyk A, Keller M, Staubach C, Bezymennyi M, Damiani AM, Osterrieder N, Groschup MH - Viruses (2013)

Sampling sites of the horse sera with focus on known West Nile virus (WNV) risk areas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814598&req=5

viruses-05-02469-f001: Sampling sites of the horse sera with focus on known West Nile virus (WNV) risk areas.
Mentions: Horse sera from 310 animals kept in 14 different Ukrainian regions were collected in 2010 and 2011 (Figure 1, Table 1). Sera were screened by a commercial WNV competition ELISA, which essentially detects flavivirus antibodies. Therefore ELISA-positive results were verified by virus neutralization tests (NT) to identify WNV or cross-reactivity specific reactions.

Bottom Line: West Nile virus (WNV) is a mosquito-borne virus of global importance.Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world.Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies.

View Article: PubMed Central - PubMed

Affiliation: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Südufer 10, D-17493 Greifswald-Insel Riems, Germany. martin.groschup@fli.bund.de.

ABSTRACT
West Nile virus (WNV) is a mosquito-borne virus of global importance. Over the last two decades, it has been responsible for significant numbers of cases of illness in humans and animals in many parts of the world. In Ukraine, WNV infections in humans and birds were first reported more than 25 years ago, yet the current epidemiological status is quite unclear. In this study, serum samples from over 300 equines were collected and screened in order to detect current WNV activity in Ukraine with the goal to estimate the risk of infection for humans and horses. Sera were tested by enzyme-linked immunosorbent assay (ELISA) and virus neutralization assay (NT) to detect WNV-specific antibodies. The results clearly revealed that WNV circulates in most of the regions from which samples were obtained, shown by a WNV seroprevalence rate of 13.5% of examined horses. This is the first topical report indicating the presence of WNV infections in horses in Ukraine, and the results of this study provide evidence of a widespread WNV circulation in this country.

Show MeSH
Related in: MedlinePlus