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Sequence heterogeneity of the ORF3 gene of porcine epidemic diarrhea viruses field samples in Fujian, China, 2010-2012.

Chen X, Zeng L, Yang J, Yu F, Ge J, Guo Q, Gao X, Song T - Viruses (2013)

Bottom Line: Twenty-seven field samples that showed positive in PEDV detection were collected from different farms of Fujian province from 2010 to 2012.Their heterogeneity was investigated by analysis of the ORF3 gene because of its potential function as a representation of virulence.The results can help to reconsider the strategy of PEDV vaccine management and prevent outbreaks of PEDV-induced diarrhea more efficiently.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China. tieyingsong@163.com.

ABSTRACT
Twenty-seven field samples that showed positive in PEDV detection were collected from different farms of Fujian province from 2010 to 2012. Their heterogeneity was investigated by analysis of the ORF3 gene because of its potential function as a representation of virulence. According to the results, six Fujian strains in Group 1 showed a different genotype with unique point mutations, which might be used in differentiation between PEDV groups and brought potential antigenic variation. P55 and five reference strains in Group 2 had a long length deletion, showing another genotype and might be involved in the variation of virulence. Phylogenetic analysis revealed that the collected Fujian strains were very distant from the vaccine development strain CV777, which might be the reason why the vaccine was inefficient to control the disease. The results can help to reconsider the strategy of PEDV vaccine management and prevent outbreaks of PEDV-induced diarrhea more efficiently.

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Related in: MedlinePlus

Alignment of amino acid sequences of ORF3 proteins of Fujian PEDV strains and reference strains. The asterisks represent the segments with no differences and not shown in this figure. The dashes represent deleted amino acids. The shadows indicate the unique substitutions of chosen strains. The boxes indicate the unique insertions or deletions of chosen strains. The underlines represent the Fujian field samples in this study.
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viruses-05-02375-f002: Alignment of amino acid sequences of ORF3 proteins of Fujian PEDV strains and reference strains. The asterisks represent the segments with no differences and not shown in this figure. The dashes represent deleted amino acids. The shadows indicate the unique substitutions of chosen strains. The boxes indicate the unique insertions or deletions of chosen strains. The underlines represent the Fujian field samples in this study.

Mentions: In terms of predicted amino acid sequence, the Subgroup 1, including six local strains, had two unique mutations, from L to S at 25 and from C to F at 107 respectively (Figure 2). It also had a unique point mutation (from V to F at 80, Figure 2), which was similar to Group 2 strains (Figure 2). P55 was found to have a long length deletion from 82 aa to the end, which existed in most of the Group 2 strains (Figure 2). No difference was found in the other 20 Fujian samples when compared to the relevant reference strains.


Sequence heterogeneity of the ORF3 gene of porcine epidemic diarrhea viruses field samples in Fujian, China, 2010-2012.

Chen X, Zeng L, Yang J, Yu F, Ge J, Guo Q, Gao X, Song T - Viruses (2013)

Alignment of amino acid sequences of ORF3 proteins of Fujian PEDV strains and reference strains. The asterisks represent the segments with no differences and not shown in this figure. The dashes represent deleted amino acids. The shadows indicate the unique substitutions of chosen strains. The boxes indicate the unique insertions or deletions of chosen strains. The underlines represent the Fujian field samples in this study.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814593&req=5

viruses-05-02375-f002: Alignment of amino acid sequences of ORF3 proteins of Fujian PEDV strains and reference strains. The asterisks represent the segments with no differences and not shown in this figure. The dashes represent deleted amino acids. The shadows indicate the unique substitutions of chosen strains. The boxes indicate the unique insertions or deletions of chosen strains. The underlines represent the Fujian field samples in this study.
Mentions: In terms of predicted amino acid sequence, the Subgroup 1, including six local strains, had two unique mutations, from L to S at 25 and from C to F at 107 respectively (Figure 2). It also had a unique point mutation (from V to F at 80, Figure 2), which was similar to Group 2 strains (Figure 2). P55 was found to have a long length deletion from 82 aa to the end, which existed in most of the Group 2 strains (Figure 2). No difference was found in the other 20 Fujian samples when compared to the relevant reference strains.

Bottom Line: Twenty-seven field samples that showed positive in PEDV detection were collected from different farms of Fujian province from 2010 to 2012.Their heterogeneity was investigated by analysis of the ORF3 gene because of its potential function as a representation of virulence.The results can help to reconsider the strategy of PEDV vaccine management and prevent outbreaks of PEDV-induced diarrhea more efficiently.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China. tieyingsong@163.com.

ABSTRACT
Twenty-seven field samples that showed positive in PEDV detection were collected from different farms of Fujian province from 2010 to 2012. Their heterogeneity was investigated by analysis of the ORF3 gene because of its potential function as a representation of virulence. According to the results, six Fujian strains in Group 1 showed a different genotype with unique point mutations, which might be used in differentiation between PEDV groups and brought potential antigenic variation. P55 and five reference strains in Group 2 had a long length deletion, showing another genotype and might be involved in the variation of virulence. Phylogenetic analysis revealed that the collected Fujian strains were very distant from the vaccine development strain CV777, which might be the reason why the vaccine was inefficient to control the disease. The results can help to reconsider the strategy of PEDV vaccine management and prevent outbreaks of PEDV-induced diarrhea more efficiently.

Show MeSH
Related in: MedlinePlus