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ABCC3 as a marker for multidrug resistance in non-small cell lung cancer.

Zhao Y, Lu H, Yan A, Yang Y, Meng Q, Sun L, Pang H, Li C, Dong X, Cai L - Sci Rep (2013)

Bottom Line: The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group.ABCC3 was one of the most up-regulated genes in the resistant group.ABCC3-positive expression correlated with lymph node involvement, advanced TNM stage, more malignant histological type, multiple-resistance to anti-cancer drugs, and reduced OS.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Internal Medical Oncology, the Affiliated Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China [2].

ABSTRACT
Multidrug resistance (MDR) contributes to the failure of chemotherapy and high mortality in non-small cell lung cancer (NSCLC). We aim to identify MDR genes that predict tumor response to chemotherapy. 199 NSCLC fresh tissue samples were tested for chemosensitivity by MTT assay. cDNA microarray was done with 5 samples with highest resistance and 6 samples with highest sensitivity. Expression of ABCC3 mRNA and protein was detected by real-time PCR and immunohistochemisty, respectively. The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group. ABCC3 was one of the most up-regulated genes in the resistant group. ABCC3-positive expression correlated with lymph node involvement, advanced TNM stage, more malignant histological type, multiple-resistance to anti-cancer drugs, and reduced OS. ABCC3 expression may serve as a marker for MDR and predictor for poor clinical outcome of NSCLC.

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Related in: MedlinePlus

ABCC3 protein expression in NSCLC.Positive (panel A–B) and negative (panel C–D) expressions of ABCC3 in patients were observed. (A, C, ×100, SP; B, D, ×200, SP).
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f3: ABCC3 protein expression in NSCLC.Positive (panel A–B) and negative (panel C–D) expressions of ABCC3 in patients were observed. (A, C, ×100, SP; B, D, ×200, SP).

Mentions: ABCC3 expression by IHC in NSCLC and its correlation with clinicopathological characteristics ABCC3 immunoreactivity was present in the cell membrane and cytoplasm of NSCLC tumor (Fig. 3). ABCC3-positive expression was observed in 63 cases including 23 with squamous cell carcinoma (SCC), 38 with adenocarcinoma (ADC), and 2 with other types (Table 1). ABCC3 expression correlated with pN status (P < 0.001), pathological TNM stage (P < 0.001) and histological type (P = 0.04).


ABCC3 as a marker for multidrug resistance in non-small cell lung cancer.

Zhao Y, Lu H, Yan A, Yang Y, Meng Q, Sun L, Pang H, Li C, Dong X, Cai L - Sci Rep (2013)

ABCC3 protein expression in NSCLC.Positive (panel A–B) and negative (panel C–D) expressions of ABCC3 in patients were observed. (A, C, ×100, SP; B, D, ×200, SP).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814586&req=5

f3: ABCC3 protein expression in NSCLC.Positive (panel A–B) and negative (panel C–D) expressions of ABCC3 in patients were observed. (A, C, ×100, SP; B, D, ×200, SP).
Mentions: ABCC3 expression by IHC in NSCLC and its correlation with clinicopathological characteristics ABCC3 immunoreactivity was present in the cell membrane and cytoplasm of NSCLC tumor (Fig. 3). ABCC3-positive expression was observed in 63 cases including 23 with squamous cell carcinoma (SCC), 38 with adenocarcinoma (ADC), and 2 with other types (Table 1). ABCC3 expression correlated with pN status (P < 0.001), pathological TNM stage (P < 0.001) and histological type (P = 0.04).

Bottom Line: The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group.ABCC3 was one of the most up-regulated genes in the resistant group.ABCC3-positive expression correlated with lymph node involvement, advanced TNM stage, more malignant histological type, multiple-resistance to anti-cancer drugs, and reduced OS.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Internal Medical Oncology, the Affiliated Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang Province, China [2].

ABSTRACT
Multidrug resistance (MDR) contributes to the failure of chemotherapy and high mortality in non-small cell lung cancer (NSCLC). We aim to identify MDR genes that predict tumor response to chemotherapy. 199 NSCLC fresh tissue samples were tested for chemosensitivity by MTT assay. cDNA microarray was done with 5 samples with highest resistance and 6 samples with highest sensitivity. Expression of ABCC3 mRNA and protein was detected by real-time PCR and immunohistochemisty, respectively. The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group. ABCC3 was one of the most up-regulated genes in the resistant group. ABCC3-positive expression correlated with lymph node involvement, advanced TNM stage, more malignant histological type, multiple-resistance to anti-cancer drugs, and reduced OS. ABCC3 expression may serve as a marker for MDR and predictor for poor clinical outcome of NSCLC.

Show MeSH
Related in: MedlinePlus