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The effect of L-arginine and L-NAME on myocardial capillary density in normal rats.

Khazaei M, Moshayedi MA, Jervekani MT, Aghili S, Montazer S, Dastjerdi RM, Hashemzehi F, Jazi HH - Adv Biomed Res (2013)

Bottom Line: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit.Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group.L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: This study evaluated the effect of L-arginine (Nitric Oxide (NO) precursor) and L-NG-Nitroarginine Methyl Ester (L-NAME) (NO synthase inhibitor) on myocardial capillary density in normal rats.

Materials and methods: EIGHTEEN MALE RATS WERE DIVIDED INTO THREE GROUPS: Group 1: Received L-NAME (10 mg/kg/day; ip), Group 2: Received L-arginine (50 mg/kg/day; ip), and Group 3 (control) received normal saline. After 3 weeks, blood samples were taken and myocardial capillary density was evaluated using immunohistochemistry method.

Results: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit. Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group. L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

Conclusion: Although L-arginine alters some serum angiogenic factors, either L-arginine or L-NAME could not improve myocardial capillary density in normal rats.

No MeSH data available.


Microscopic view of the heart muscle stained with anti-CD31 antibody using immunohistochemistry method in (a) Control, (b) recipients of L-arginine, (c) recipients of L-NAME. Images are with magnification of ×400
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Figure 4: Microscopic view of the heart muscle stained with anti-CD31 antibody using immunohistochemistry method in (a) Control, (b) recipients of L-arginine, (c) recipients of L-NAME. Images are with magnification of ×400

Mentions: Neither L-arginine nor L-NAME made a significant difference in capillary density and coronary angiogenesis [Figure 3]. Example of IHC slides prepared from the hearts of animals are presented in Figure 4.


The effect of L-arginine and L-NAME on myocardial capillary density in normal rats.

Khazaei M, Moshayedi MA, Jervekani MT, Aghili S, Montazer S, Dastjerdi RM, Hashemzehi F, Jazi HH - Adv Biomed Res (2013)

Microscopic view of the heart muscle stained with anti-CD31 antibody using immunohistochemistry method in (a) Control, (b) recipients of L-arginine, (c) recipients of L-NAME. Images are with magnification of ×400
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814566&req=5

Figure 4: Microscopic view of the heart muscle stained with anti-CD31 antibody using immunohistochemistry method in (a) Control, (b) recipients of L-arginine, (c) recipients of L-NAME. Images are with magnification of ×400
Mentions: Neither L-arginine nor L-NAME made a significant difference in capillary density and coronary angiogenesis [Figure 3]. Example of IHC slides prepared from the hearts of animals are presented in Figure 4.

Bottom Line: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit.Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group.L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: This study evaluated the effect of L-arginine (Nitric Oxide (NO) precursor) and L-NG-Nitroarginine Methyl Ester (L-NAME) (NO synthase inhibitor) on myocardial capillary density in normal rats.

Materials and methods: EIGHTEEN MALE RATS WERE DIVIDED INTO THREE GROUPS: Group 1: Received L-NAME (10 mg/kg/day; ip), Group 2: Received L-arginine (50 mg/kg/day; ip), and Group 3 (control) received normal saline. After 3 weeks, blood samples were taken and myocardial capillary density was evaluated using immunohistochemistry method.

Results: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit. Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group. L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

Conclusion: Although L-arginine alters some serum angiogenic factors, either L-arginine or L-NAME could not improve myocardial capillary density in normal rats.

No MeSH data available.