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The effect of L-arginine and L-NAME on myocardial capillary density in normal rats.

Khazaei M, Moshayedi MA, Jervekani MT, Aghili S, Montazer S, Dastjerdi RM, Hashemzehi F, Jazi HH - Adv Biomed Res (2013)

Bottom Line: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit.Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group.L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: This study evaluated the effect of L-arginine (Nitric Oxide (NO) precursor) and L-NG-Nitroarginine Methyl Ester (L-NAME) (NO synthase inhibitor) on myocardial capillary density in normal rats.

Materials and methods: EIGHTEEN MALE RATS WERE DIVIDED INTO THREE GROUPS: Group 1: Received L-NAME (10 mg/kg/day; ip), Group 2: Received L-arginine (50 mg/kg/day; ip), and Group 3 (control) received normal saline. After 3 weeks, blood samples were taken and myocardial capillary density was evaluated using immunohistochemistry method.

Results: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit. Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group. L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

Conclusion: Although L-arginine alters some serum angiogenic factors, either L-arginine or L-NAME could not improve myocardial capillary density in normal rats.

No MeSH data available.


Serum VEGF level (pg/ml) at the end of study (*P < 0.05 compared with other groups)
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Figure 2: Serum VEGF level (pg/ml) at the end of study (*P < 0.05 compared with other groups)

Mentions: At the beginning of the experiment, serum VEGF level in the three groups was not significantly different between groups (P > 0.05), but at the end of study, serum level of VEGF in the control group was 78.22 ± 5.14 pg/ml. L-arginine significantly increased this level to 353.01 ± 7.03 pg/ml (P < 0.05), while L-NAME could not alter significant differences in serum VEGF level [Figure 2].


The effect of L-arginine and L-NAME on myocardial capillary density in normal rats.

Khazaei M, Moshayedi MA, Jervekani MT, Aghili S, Montazer S, Dastjerdi RM, Hashemzehi F, Jazi HH - Adv Biomed Res (2013)

Serum VEGF level (pg/ml) at the end of study (*P < 0.05 compared with other groups)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814566&req=5

Figure 2: Serum VEGF level (pg/ml) at the end of study (*P < 0.05 compared with other groups)
Mentions: At the beginning of the experiment, serum VEGF level in the three groups was not significantly different between groups (P > 0.05), but at the end of study, serum level of VEGF in the control group was 78.22 ± 5.14 pg/ml. L-arginine significantly increased this level to 353.01 ± 7.03 pg/ml (P < 0.05), while L-NAME could not alter significant differences in serum VEGF level [Figure 2].

Bottom Line: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit.Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group.L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: This study evaluated the effect of L-arginine (Nitric Oxide (NO) precursor) and L-NG-Nitroarginine Methyl Ester (L-NAME) (NO synthase inhibitor) on myocardial capillary density in normal rats.

Materials and methods: EIGHTEEN MALE RATS WERE DIVIDED INTO THREE GROUPS: Group 1: Received L-NAME (10 mg/kg/day; ip), Group 2: Received L-arginine (50 mg/kg/day; ip), and Group 3 (control) received normal saline. After 3 weeks, blood samples were taken and myocardial capillary density was evaluated using immunohistochemistry method.

Results: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit. Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group. L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

Conclusion: Although L-arginine alters some serum angiogenic factors, either L-arginine or L-NAME could not improve myocardial capillary density in normal rats.

No MeSH data available.