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A comparison of peroxisome proliferator-activated receptor-α agonist and antagonist on human umbilical vein endothelial cells angiogenesis.

Dana N, Javanmard SH, Fazilati M, Pilehvarian AA - Adv Biomed Res (2013)

Bottom Line: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα).These results showed that PPARα agonist is effective in suppression of angiogenesis.Further studies are needed to confirm these results in in vivo studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα). In the current study, we compared the effects of PPARα agonist and antagonist on human umbilical vein endothelial cells (HUVECs) angiogenesis with matrigel assay.

Materials and methods: HUVECs (1 × 10(5) cells/well) treated with PPARα agonist (fenofibrate) and antagonist (GW6471) were cultured on matrigel for 24 h. Treated cells were stained with calcein and investigated by fluorescent microscopy. The obtained images were also analyzed by AngioQuant software. Finally, the data were analyzed using SPSS 15 software, Kruskal-Wallis and one way ANOVA.

Results: Statistical analysis showed that fenofibrate significantly inhibit the tube formation (size, length, junction) (P < 0.05) but there was a trend to increased angiogenesis in GW6471 treated group (P > 0.05).

Conclusion: These results showed that PPARα agonist is effective in suppression of angiogenesis. Further studies are needed to confirm these results in in vivo studies.

No MeSH data available.


Related in: MedlinePlus

The effect of fenofibrate and GW6471 on angiogenesis by HUVECs. Quantitative analysis of mean tube length from three independent experiments are shown. *Significantly different from positive control group. #Significantly different from negative control group
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Figure 3: The effect of fenofibrate and GW6471 on angiogenesis by HUVECs. Quantitative analysis of mean tube length from three independent experiments are shown. *Significantly different from positive control group. #Significantly different from negative control group

Mentions: Our results showed that fenofibrate significantly decreased the size [Figure 2], length [Figure 3] and junction of tubes [Figure 4] compared to negative and positive control groups (P < 0.05).


A comparison of peroxisome proliferator-activated receptor-α agonist and antagonist on human umbilical vein endothelial cells angiogenesis.

Dana N, Javanmard SH, Fazilati M, Pilehvarian AA - Adv Biomed Res (2013)

The effect of fenofibrate and GW6471 on angiogenesis by HUVECs. Quantitative analysis of mean tube length from three independent experiments are shown. *Significantly different from positive control group. #Significantly different from negative control group
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814543&req=5

Figure 3: The effect of fenofibrate and GW6471 on angiogenesis by HUVECs. Quantitative analysis of mean tube length from three independent experiments are shown. *Significantly different from positive control group. #Significantly different from negative control group
Mentions: Our results showed that fenofibrate significantly decreased the size [Figure 2], length [Figure 3] and junction of tubes [Figure 4] compared to negative and positive control groups (P < 0.05).

Bottom Line: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα).These results showed that PPARα agonist is effective in suppression of angiogenesis.Further studies are needed to confirm these results in in vivo studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα). In the current study, we compared the effects of PPARα agonist and antagonist on human umbilical vein endothelial cells (HUVECs) angiogenesis with matrigel assay.

Materials and methods: HUVECs (1 × 10(5) cells/well) treated with PPARα agonist (fenofibrate) and antagonist (GW6471) were cultured on matrigel for 24 h. Treated cells were stained with calcein and investigated by fluorescent microscopy. The obtained images were also analyzed by AngioQuant software. Finally, the data were analyzed using SPSS 15 software, Kruskal-Wallis and one way ANOVA.

Results: Statistical analysis showed that fenofibrate significantly inhibit the tube formation (size, length, junction) (P < 0.05) but there was a trend to increased angiogenesis in GW6471 treated group (P > 0.05).

Conclusion: These results showed that PPARα agonist is effective in suppression of angiogenesis. Further studies are needed to confirm these results in in vivo studies.

No MeSH data available.


Related in: MedlinePlus