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A comparison of peroxisome proliferator-activated receptor-α agonist and antagonist on human umbilical vein endothelial cells angiogenesis.

Dana N, Javanmard SH, Fazilati M, Pilehvarian AA - Adv Biomed Res (2013)

Bottom Line: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα).These results showed that PPARα agonist is effective in suppression of angiogenesis.Further studies are needed to confirm these results in in vivo studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα). In the current study, we compared the effects of PPARα agonist and antagonist on human umbilical vein endothelial cells (HUVECs) angiogenesis with matrigel assay.

Materials and methods: HUVECs (1 × 10(5) cells/well) treated with PPARα agonist (fenofibrate) and antagonist (GW6471) were cultured on matrigel for 24 h. Treated cells were stained with calcein and investigated by fluorescent microscopy. The obtained images were also analyzed by AngioQuant software. Finally, the data were analyzed using SPSS 15 software, Kruskal-Wallis and one way ANOVA.

Results: Statistical analysis showed that fenofibrate significantly inhibit the tube formation (size, length, junction) (P < 0.05) but there was a trend to increased angiogenesis in GW6471 treated group (P > 0.05).

Conclusion: These results showed that PPARα agonist is effective in suppression of angiogenesis. Further studies are needed to confirm these results in in vivo studies.

No MeSH data available.


Related in: MedlinePlus

The effect of PPARα agonist (fenofibrate) and antagonis (GW6471) on tube formation of HUVECs. HUVECs were plated on a well coated with 100 μl of Matrigel basement membrane matrix. After they were treated for 24 h, the cells were dyed and photographed with a Nikon camera attached to a fluorescent microscope at ×10 magnification
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Figure 1: The effect of PPARα agonist (fenofibrate) and antagonis (GW6471) on tube formation of HUVECs. HUVECs were plated on a well coated with 100 μl of Matrigel basement membrane matrix. After they were treated for 24 h, the cells were dyed and photographed with a Nikon camera attached to a fluorescent microscope at ×10 magnification

Mentions: To verify the anti-angiogenic activity of fenofibrate, the cells treatment started at the time of seeding HUVEC on to matrigel, and endothelial cells tube formation was observed over a period of time. Fenofibrate significantly suppressed the formation of tube-like structures (P < 0.05) [Figure 1].


A comparison of peroxisome proliferator-activated receptor-α agonist and antagonist on human umbilical vein endothelial cells angiogenesis.

Dana N, Javanmard SH, Fazilati M, Pilehvarian AA - Adv Biomed Res (2013)

The effect of PPARα agonist (fenofibrate) and antagonis (GW6471) on tube formation of HUVECs. HUVECs were plated on a well coated with 100 μl of Matrigel basement membrane matrix. After they were treated for 24 h, the cells were dyed and photographed with a Nikon camera attached to a fluorescent microscope at ×10 magnification
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814543&req=5

Figure 1: The effect of PPARα agonist (fenofibrate) and antagonis (GW6471) on tube formation of HUVECs. HUVECs were plated on a well coated with 100 μl of Matrigel basement membrane matrix. After they were treated for 24 h, the cells were dyed and photographed with a Nikon camera attached to a fluorescent microscope at ×10 magnification
Mentions: To verify the anti-angiogenic activity of fenofibrate, the cells treatment started at the time of seeding HUVEC on to matrigel, and endothelial cells tube formation was observed over a period of time. Fenofibrate significantly suppressed the formation of tube-like structures (P < 0.05) [Figure 1].

Bottom Line: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα).These results showed that PPARα agonist is effective in suppression of angiogenesis.Further studies are needed to confirm these results in in vivo studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα). In the current study, we compared the effects of PPARα agonist and antagonist on human umbilical vein endothelial cells (HUVECs) angiogenesis with matrigel assay.

Materials and methods: HUVECs (1 × 10(5) cells/well) treated with PPARα agonist (fenofibrate) and antagonist (GW6471) were cultured on matrigel for 24 h. Treated cells were stained with calcein and investigated by fluorescent microscopy. The obtained images were also analyzed by AngioQuant software. Finally, the data were analyzed using SPSS 15 software, Kruskal-Wallis and one way ANOVA.

Results: Statistical analysis showed that fenofibrate significantly inhibit the tube formation (size, length, junction) (P < 0.05) but there was a trend to increased angiogenesis in GW6471 treated group (P > 0.05).

Conclusion: These results showed that PPARα agonist is effective in suppression of angiogenesis. Further studies are needed to confirm these results in in vivo studies.

No MeSH data available.


Related in: MedlinePlus