Detection of potential TNA and RNA nucleoside precursors in a prebiotic mixture by pure shift diffusion-ordered NMR spectroscopy.
Bottom Line: One potential avenue is the combination of pure shift methodology, in which NMR spectra are measured with greatly improved resolution by suppressing multiplet structure, with diffusion-ordered spectroscopy, in which NMR signals from different species are distinguished through their different rates of diffusion.Such a combination has the added advantage of working with intact mixtures, allowing analyses to be carried out without perturbing mixtures in which chemical entities are part of a network of reactions in equilibrium.The direct formation of potential RNA and TNA nucleoside precursors, amongst other adducts, was observed.
Affiliation: School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. firstname.lastname@example.orgShow MeSH
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Mentions: The main obstacle to NMR analysis of this type of reaction mixture is lack of resolving power, so it represents an ideal test for a new class of experiment that collapses homonuclear multiplet structure to give a single spectral line per chemical site, and has hence been termed “pure shift” NMR.[31, 33, 41] One of the most effective of these methods for broadband homonuclear decoupling is based on the work of Zangger and Sterk and has been incorporated into a variety of uni- and multidimensional experiments such as DOSY and TOCSY (Figure 3 b).[31, 32, 43] Reported applications of such methods have hitherto been limited to simple samples containing no more than a few chemical species of comparable concentration. Here, Zangger–Sterk methods are applied to a real and highly complex problem in mixture analysis.
Affiliation: School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. email@example.com