Detection of potential TNA and RNA nucleoside precursors in a prebiotic mixture by pure shift diffusion-ordered NMR spectroscopy.
Bottom Line: One potential avenue is the combination of pure shift methodology, in which NMR spectra are measured with greatly improved resolution by suppressing multiplet structure, with diffusion-ordered spectroscopy, in which NMR signals from different species are distinguished through their different rates of diffusion.Such a combination has the added advantage of working with intact mixtures, allowing analyses to be carried out without perturbing mixtures in which chemical entities are part of a network of reactions in equilibrium.The direct formation of potential RNA and TNA nucleoside precursors, amongst other adducts, was observed.
Affiliation: School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. email@example.comShow MeSH
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Mentions: The seminal contributions of Eschenmoser and his co-workers on the chemical etiology of the nucleic acid structure led to the discovery of various informational base-pairing systems that are constitutionally related to RNA.[9–11] Threose nucleic acid (TNA), an oligonucleotide consisting of a four-carbon threofuranosyl sugar, a five covalent bond trans-diaxial phosphodiester backbone, and only three stereogenic centres, has attracted particular interest (Figure 1). The structural simplifications of TNA (relative to RNA), and its ability to Watson–Crick base-pair not only to complementary strands of TNA, but also to RNA and DNA, have led to the suggestion that TNA may have predated RNA in the origin of life.
Affiliation: School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. firstname.lastname@example.org