Limits...
Detection of potential TNA and RNA nucleoside precursors in a prebiotic mixture by pure shift diffusion-ordered NMR spectroscopy.

Islam S, Aguilar JA, Powner MW, Nilsson M, Morris GA, Sutherland JD - Chemistry (2013)

Bottom Line: One potential avenue is the combination of pure shift methodology, in which NMR spectra are measured with greatly improved resolution by suppressing multiplet structure, with diffusion-ordered spectroscopy, in which NMR signals from different species are distinguished through their different rates of diffusion.Such a combination has the added advantage of working with intact mixtures, allowing analyses to be carried out without perturbing mixtures in which chemical entities are part of a network of reactions in equilibrium.The direct formation of potential RNA and TNA nucleoside precursors, amongst other adducts, was observed.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. saidul.islam@qmul.ac.uk

Show MeSH

Related in: MedlinePlus

Idealised pairing conformations, constitutions and configurations of RNA and TNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3814424&req=5

fig01: Idealised pairing conformations, constitutions and configurations of RNA and TNA.

Mentions: The seminal contributions of Eschenmoser and his co-workers on the chemical etiology of the nucleic acid structure led to the discovery of various informational base-pairing systems that are constitutionally related to RNA.[9–11] Threose nucleic acid (TNA), an oligonucleotide consisting of a four-carbon threofuranosyl sugar, a five covalent bond trans-diaxial phosphodiester backbone, and only three stereogenic centres, has attracted particular interest (Figure 1).[12] The structural simplifications of TNA (relative to RNA), and its ability to Watson–Crick base-pair not only to complementary strands of TNA, but also to RNA and DNA, have led to the suggestion that TNA may have predated RNA in the origin of life.[13]


Detection of potential TNA and RNA nucleoside precursors in a prebiotic mixture by pure shift diffusion-ordered NMR spectroscopy.

Islam S, Aguilar JA, Powner MW, Nilsson M, Morris GA, Sutherland JD - Chemistry (2013)

Idealised pairing conformations, constitutions and configurations of RNA and TNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814424&req=5

fig01: Idealised pairing conformations, constitutions and configurations of RNA and TNA.
Mentions: The seminal contributions of Eschenmoser and his co-workers on the chemical etiology of the nucleic acid structure led to the discovery of various informational base-pairing systems that are constitutionally related to RNA.[9–11] Threose nucleic acid (TNA), an oligonucleotide consisting of a four-carbon threofuranosyl sugar, a five covalent bond trans-diaxial phosphodiester backbone, and only three stereogenic centres, has attracted particular interest (Figure 1).[12] The structural simplifications of TNA (relative to RNA), and its ability to Watson–Crick base-pair not only to complementary strands of TNA, but also to RNA and DNA, have led to the suggestion that TNA may have predated RNA in the origin of life.[13]

Bottom Line: One potential avenue is the combination of pure shift methodology, in which NMR spectra are measured with greatly improved resolution by suppressing multiplet structure, with diffusion-ordered spectroscopy, in which NMR signals from different species are distinguished through their different rates of diffusion.Such a combination has the added advantage of working with intact mixtures, allowing analyses to be carried out without perturbing mixtures in which chemical entities are part of a network of reactions in equilibrium.The direct formation of potential RNA and TNA nucleoside precursors, amongst other adducts, was observed.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry, University of Manchester, Oxford Road, Manchester, M13 9PL, UK. saidul.islam@qmul.ac.uk

Show MeSH
Related in: MedlinePlus