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Chlorophyll breakdown in senescent banana leaves: catabolism reprogrammed for biosynthesis of persistent blue fluorescent tetrapyrroles.

Vergeiner C, Banala S, Kräutler B - Chemistry (2013)

Bottom Line: Amazingly, in the leaves of banana plants, persistent hmFCCs were identified that accounted for about 80 % of the chlorophyll broken down, and yellow leaves of M. acuminata display a strong blue luminescence.The structures of eight hmFCCs from banana leaves were analyzed by spectroscopic means.As expressed earlier in related studies, the present findings call for attention, as to still elusive biological roles of these linear tetrapyrroles.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organic Chemistry & Center for Molecular Biosciences, University of Innsbruck, 6020 Innsbruck (Austria).

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The four major FCCs in senescent banana leaves, Ma-FCC-61, Ma-FCC-63, Ma-FCC-64 and Ma-FCC-69, are hmFCCs and have similar UV/Vis spectra (top). The CD-spectra (bottom) of the glucosyl-carrying FCCs are similar, the digalactosyl-substituted Ma-FCC-61 deviates slightly; spectra of FCCs in MeOH (see the Experimental Section for details).
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fig04: The four major FCCs in senescent banana leaves, Ma-FCC-61, Ma-FCC-63, Ma-FCC-64 and Ma-FCC-69, are hmFCCs and have similar UV/Vis spectra (top). The CD-spectra (bottom) of the glucosyl-carrying FCCs are similar, the digalactosyl-substituted Ma-FCC-61 deviates slightly; spectra of FCCs in MeOH (see the Experimental Section for details).

Mentions: The two slightly less polar FCCs, Ma-FCC-63 and Ma-FCC-64, could be separated (in part) by preparative HPLC in MeOH/H2O (see the Experimental Section). The first separation was incomplete due to partial interconversion of these isomeric hmFCCs (see below). For the second, final purification step corresponding precautions were taken to prevent isomerization and possible transesterification (with methanol) by changing the solvent system to ACN/H2O and by running HPLC with shorter on-column times. The FCC containing fractions were directly frozen in liquid nitrogen, stored at −80 °C to avoid isomerization. The samples were lyophilized, to yield 0.79 mg (0.98 μmol) of analytical pure Ma-FCC-63, and 0.63 mg (0.77 μmol) of Ma-FCC-64, to be used for spectroscopic analyses. The UV/Vis spectra of the two FCCs as well as their CD spectra showed considerable similarities; these spectra were also comparable to those of Ma-FCC-69 (Figure 4).


Chlorophyll breakdown in senescent banana leaves: catabolism reprogrammed for biosynthesis of persistent blue fluorescent tetrapyrroles.

Vergeiner C, Banala S, Kräutler B - Chemistry (2013)

The four major FCCs in senescent banana leaves, Ma-FCC-61, Ma-FCC-63, Ma-FCC-64 and Ma-FCC-69, are hmFCCs and have similar UV/Vis spectra (top). The CD-spectra (bottom) of the glucosyl-carrying FCCs are similar, the digalactosyl-substituted Ma-FCC-61 deviates slightly; spectra of FCCs in MeOH (see the Experimental Section for details).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814416&req=5

fig04: The four major FCCs in senescent banana leaves, Ma-FCC-61, Ma-FCC-63, Ma-FCC-64 and Ma-FCC-69, are hmFCCs and have similar UV/Vis spectra (top). The CD-spectra (bottom) of the glucosyl-carrying FCCs are similar, the digalactosyl-substituted Ma-FCC-61 deviates slightly; spectra of FCCs in MeOH (see the Experimental Section for details).
Mentions: The two slightly less polar FCCs, Ma-FCC-63 and Ma-FCC-64, could be separated (in part) by preparative HPLC in MeOH/H2O (see the Experimental Section). The first separation was incomplete due to partial interconversion of these isomeric hmFCCs (see below). For the second, final purification step corresponding precautions were taken to prevent isomerization and possible transesterification (with methanol) by changing the solvent system to ACN/H2O and by running HPLC with shorter on-column times. The FCC containing fractions were directly frozen in liquid nitrogen, stored at −80 °C to avoid isomerization. The samples were lyophilized, to yield 0.79 mg (0.98 μmol) of analytical pure Ma-FCC-63, and 0.63 mg (0.77 μmol) of Ma-FCC-64, to be used for spectroscopic analyses. The UV/Vis spectra of the two FCCs as well as their CD spectra showed considerable similarities; these spectra were also comparable to those of Ma-FCC-69 (Figure 4).

Bottom Line: Amazingly, in the leaves of banana plants, persistent hmFCCs were identified that accounted for about 80 % of the chlorophyll broken down, and yellow leaves of M. acuminata display a strong blue luminescence.The structures of eight hmFCCs from banana leaves were analyzed by spectroscopic means.As expressed earlier in related studies, the present findings call for attention, as to still elusive biological roles of these linear tetrapyrroles.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organic Chemistry & Center for Molecular Biosciences, University of Innsbruck, 6020 Innsbruck (Austria).

Show MeSH
Related in: MedlinePlus