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Chlorophyll breakdown in senescent banana leaves: catabolism reprogrammed for biosynthesis of persistent blue fluorescent tetrapyrroles.

Vergeiner C, Banala S, Kräutler B - Chemistry (2013)

Bottom Line: Amazingly, in the leaves of banana plants, persistent hmFCCs were identified that accounted for about 80 % of the chlorophyll broken down, and yellow leaves of M. acuminata display a strong blue luminescence.The structures of eight hmFCCs from banana leaves were analyzed by spectroscopic means.As expressed earlier in related studies, the present findings call for attention, as to still elusive biological roles of these linear tetrapyrroles.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organic Chemistry & Center for Molecular Biosciences, University of Innsbruck, 6020 Innsbruck (Austria).

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Quantification of chlorophylls and Ma-FCCs in green and in senescent banana leaves. The total amounts of chlorophylls a and b, and of FCCs are depicted as a function of the degree of senescence of the leaf samples (analysis of extracts from three samples with similar extent of yellowing). The chlorophyll content was calculated from UV/Vis spectra,[27] the amount of FCCs from FCC fractions isolated raw by semipreparative HPLC (data are normalized with respect to the chlorophyll content of green leaves).
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fig03: Quantification of chlorophylls and Ma-FCCs in green and in senescent banana leaves. The total amounts of chlorophylls a and b, and of FCCs are depicted as a function of the degree of senescence of the leaf samples (analysis of extracts from three samples with similar extent of yellowing). The chlorophyll content was calculated from UV/Vis spectra,[27] the amount of FCCs from FCC fractions isolated raw by semipreparative HPLC (data are normalized with respect to the chlorophyll content of green leaves).

Mentions: Quantification of tetrapyrroles in senescent banana leaves: In M. acuminata leaves, freshly harvested at different stages of senescence, the amounts of chlorophylls (a and b) and of fluorescent chlorophyll catabolites (FCCs) were determined quantitatively (Figure 3). For this purpose, green, greenish-yellow, yellow-greenish, shiny yellow and yellow-brownish areas were cut out from different banana leaves and extracted with methanol. Quantitative UV/Vis spectroscopic measurements of the filtered extracts of green leaves and analysis for their chlorophyll content[27] indicated the leaves to contain 61.3(±5.3) nmol cm−2 of chlorophyll a and b (n=3).


Chlorophyll breakdown in senescent banana leaves: catabolism reprogrammed for biosynthesis of persistent blue fluorescent tetrapyrroles.

Vergeiner C, Banala S, Kräutler B - Chemistry (2013)

Quantification of chlorophylls and Ma-FCCs in green and in senescent banana leaves. The total amounts of chlorophylls a and b, and of FCCs are depicted as a function of the degree of senescence of the leaf samples (analysis of extracts from three samples with similar extent of yellowing). The chlorophyll content was calculated from UV/Vis spectra,[27] the amount of FCCs from FCC fractions isolated raw by semipreparative HPLC (data are normalized with respect to the chlorophyll content of green leaves).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814416&req=5

fig03: Quantification of chlorophylls and Ma-FCCs in green and in senescent banana leaves. The total amounts of chlorophylls a and b, and of FCCs are depicted as a function of the degree of senescence of the leaf samples (analysis of extracts from three samples with similar extent of yellowing). The chlorophyll content was calculated from UV/Vis spectra,[27] the amount of FCCs from FCC fractions isolated raw by semipreparative HPLC (data are normalized with respect to the chlorophyll content of green leaves).
Mentions: Quantification of tetrapyrroles in senescent banana leaves: In M. acuminata leaves, freshly harvested at different stages of senescence, the amounts of chlorophylls (a and b) and of fluorescent chlorophyll catabolites (FCCs) were determined quantitatively (Figure 3). For this purpose, green, greenish-yellow, yellow-greenish, shiny yellow and yellow-brownish areas were cut out from different banana leaves and extracted with methanol. Quantitative UV/Vis spectroscopic measurements of the filtered extracts of green leaves and analysis for their chlorophyll content[27] indicated the leaves to contain 61.3(±5.3) nmol cm−2 of chlorophyll a and b (n=3).

Bottom Line: Amazingly, in the leaves of banana plants, persistent hmFCCs were identified that accounted for about 80 % of the chlorophyll broken down, and yellow leaves of M. acuminata display a strong blue luminescence.The structures of eight hmFCCs from banana leaves were analyzed by spectroscopic means.As expressed earlier in related studies, the present findings call for attention, as to still elusive biological roles of these linear tetrapyrroles.

View Article: PubMed Central - PubMed

Affiliation: Institute of Organic Chemistry & Center for Molecular Biosciences, University of Innsbruck, 6020 Innsbruck (Austria).

Show MeSH
Related in: MedlinePlus