Characterization of fungal RTG2 genes in retrograde signaling of Saccharomyces cerevisiae.
Bottom Line: Changes in the functional status of mitochondria result in the transcriptional activation of a subset of nuclear-encoded genes in a process referred to as retrograde signaling.Our findings show that all four Rtg2p homologs are functional upon activation of retrograde signaling, although their degree of complementation varied.In addition, all Rtg2p homologs showed a marked reduction in Mks1p binding, which may contribute to their altered responses to retrograde signaling.
Affiliation: Department of Biological Sciences, Mississippi State University, P.O. Box GY, Starkville, MS 39762, USA.Show MeSH
Related in: MedlinePlus
Mentions: The current model for activation of retrograde signaling proposes that a dynamic interaction exists between Mks1p and Bmh1p and Mks1p and Rtg2p (Liu et al., 2003; Ferreira et al., 2005). To assess whether the decreased interaction between Mks1p and Rtg2p homologs was paralleled by an increased interaction between Mks1p and Bmh1p, a co-immunoprecipitation approach was taken. Mks1p–Bmh1p interaction was measured under both basal (+ glutamate) and inducing (- glutamate) conditions. Cells grown in the presence of glutamate showed a similar interaction affinity between Mks1p and Bmh1p irrespective of the expressed Rtg2p homolog (Fig. 5 and Table S4). Given the inverse relationship dynamics between Mks1p–Rtg2p and Mks1p–Bmh1p complex formation, cells grown under inducing conditions should decrease Mks1p–Bmh1p complex formation to facilitate an increased Mks1p–Rtg2p interaction (Liu et al., 2003). Interestingly, all Rtg2p homologs showed a stronger interaction between Mks1p and Bmh1p compared with that detected for S. cerevisiae RTG2 expressing cells under inducing conditions.
Affiliation: Department of Biological Sciences, Mississippi State University, P.O. Box GY, Starkville, MS 39762, USA.