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Mediated plastid RNA editing in plant immunity.

García-Andrade J, Ramírez V, López A, Vera P - PLoS Pathog. (2013)

Bottom Line: Furthermore, we observed that following a pathogenic challenge, wild type plants respond with editing inhibition of ndhB transcript.In parallel, rapid destabilization of the plastidial NDH complex is also observed in the plant following perception of a pathogenic cue.Therefore, NDH complex activity and plant immunity appear as interlinked processes.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C, Ciudad Politécnica de la Innovación, Ingeniero Fausto Elio, Valencia, Spain.

ABSTRACT
Plant regulatory circuits coordinating nuclear and plastid gene expression have evolved in response to external stimuli. RNA editing is one of such control mechanisms. We determined the Arabidopsis nuclear-encoded homeodomain-containing protein OCP3 is incorporated into the chloroplast, and contributes to control over the extent of ndhB transcript editing. ndhB encodes the B subunit of the chloroplast NADH dehydrogenase-like complex (NDH) involved in cyclic electron flow (CEF) around photosystem I. In ocp3 mutant strains, ndhB editing efficiency decays, CEF is impaired and disease resistance to fungal pathogens substantially enhanced, a process recapitulated in plants defective in editing plastid RNAs encoding NDH complex subunits due to mutations in previously described nuclear-encoded pentatricopeptide-related proteins (i.e. CRR21, CRR2). Furthermore, we observed that following a pathogenic challenge, wild type plants respond with editing inhibition of ndhB transcript. In parallel, rapid destabilization of the plastidial NDH complex is also observed in the plant following perception of a pathogenic cue. Therefore, NDH complex activity and plant immunity appear as interlinked processes.

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OCP3 co-localizes with pTAC2.(A) Chloroplast localization pattern of PGL34-YFP, PEND-RFP, WHIRLY-GFP, pTAC2-YFP and OCP3-mCHERRY in protoplasts from N. benthamiana evaluated by confocal microscopy. (B) Co-localization patterns of OCP3 with each of the proteins shown in (A). YFP- and GFP-specific fluorescence is shown in green, RFP- and mCHERRY-specific fluorescence is shown in red and chlorophyll-derived fluorescence is shown in blue.
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ppat-1003713-g002: OCP3 co-localizes with pTAC2.(A) Chloroplast localization pattern of PGL34-YFP, PEND-RFP, WHIRLY-GFP, pTAC2-YFP and OCP3-mCHERRY in protoplasts from N. benthamiana evaluated by confocal microscopy. (B) Co-localization patterns of OCP3 with each of the proteins shown in (A). YFP- and GFP-specific fluorescence is shown in green, RFP- and mCHERRY-specific fluorescence is shown in red and chlorophyll-derived fluorescence is shown in blue.

Mentions: OCP3-YFP distribution within the chloroplast showed protein accumulated in the form of speckles or punctate patterns. We noted similarities among proteins targeting different plastid structures or molecules, including plastoglobules (i.e. PGL34, [29]), plastid nucleoids (PEND, [30]), targeting associated with introns containing RNAs (i.e. WHIRLY, [31]) and with RNAs undergoing editing (i.e. pTAC2) (Figure 2A). We co-expressed the OCP3-mCherry protein with PGL34-YFP, PEND-RFP, WHIRLY-GFP, or pTAC2-YFP in protoplasts to demonstrate possible co-localizations. OCP3-mCherry fluorescence overlapped consistently with the fluorescence derived from the pentatricopeptide repeat (PPR) protein pTAC2-YFP (Figure 2B).


Mediated plastid RNA editing in plant immunity.

García-Andrade J, Ramírez V, López A, Vera P - PLoS Pathog. (2013)

OCP3 co-localizes with pTAC2.(A) Chloroplast localization pattern of PGL34-YFP, PEND-RFP, WHIRLY-GFP, pTAC2-YFP and OCP3-mCHERRY in protoplasts from N. benthamiana evaluated by confocal microscopy. (B) Co-localization patterns of OCP3 with each of the proteins shown in (A). YFP- and GFP-specific fluorescence is shown in green, RFP- and mCHERRY-specific fluorescence is shown in red and chlorophyll-derived fluorescence is shown in blue.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814343&req=5

ppat-1003713-g002: OCP3 co-localizes with pTAC2.(A) Chloroplast localization pattern of PGL34-YFP, PEND-RFP, WHIRLY-GFP, pTAC2-YFP and OCP3-mCHERRY in protoplasts from N. benthamiana evaluated by confocal microscopy. (B) Co-localization patterns of OCP3 with each of the proteins shown in (A). YFP- and GFP-specific fluorescence is shown in green, RFP- and mCHERRY-specific fluorescence is shown in red and chlorophyll-derived fluorescence is shown in blue.
Mentions: OCP3-YFP distribution within the chloroplast showed protein accumulated in the form of speckles or punctate patterns. We noted similarities among proteins targeting different plastid structures or molecules, including plastoglobules (i.e. PGL34, [29]), plastid nucleoids (PEND, [30]), targeting associated with introns containing RNAs (i.e. WHIRLY, [31]) and with RNAs undergoing editing (i.e. pTAC2) (Figure 2A). We co-expressed the OCP3-mCherry protein with PGL34-YFP, PEND-RFP, WHIRLY-GFP, or pTAC2-YFP in protoplasts to demonstrate possible co-localizations. OCP3-mCherry fluorescence overlapped consistently with the fluorescence derived from the pentatricopeptide repeat (PPR) protein pTAC2-YFP (Figure 2B).

Bottom Line: Furthermore, we observed that following a pathogenic challenge, wild type plants respond with editing inhibition of ndhB transcript.In parallel, rapid destabilization of the plastidial NDH complex is also observed in the plant following perception of a pathogenic cue.Therefore, NDH complex activity and plant immunity appear as interlinked processes.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C, Ciudad Politécnica de la Innovación, Ingeniero Fausto Elio, Valencia, Spain.

ABSTRACT
Plant regulatory circuits coordinating nuclear and plastid gene expression have evolved in response to external stimuli. RNA editing is one of such control mechanisms. We determined the Arabidopsis nuclear-encoded homeodomain-containing protein OCP3 is incorporated into the chloroplast, and contributes to control over the extent of ndhB transcript editing. ndhB encodes the B subunit of the chloroplast NADH dehydrogenase-like complex (NDH) involved in cyclic electron flow (CEF) around photosystem I. In ocp3 mutant strains, ndhB editing efficiency decays, CEF is impaired and disease resistance to fungal pathogens substantially enhanced, a process recapitulated in plants defective in editing plastid RNAs encoding NDH complex subunits due to mutations in previously described nuclear-encoded pentatricopeptide-related proteins (i.e. CRR21, CRR2). Furthermore, we observed that following a pathogenic challenge, wild type plants respond with editing inhibition of ndhB transcript. In parallel, rapid destabilization of the plastidial NDH complex is also observed in the plant following perception of a pathogenic cue. Therefore, NDH complex activity and plant immunity appear as interlinked processes.

Show MeSH
Related in: MedlinePlus