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Effect of ionizing radiation on human skeletal muscle precursor cells.

Jurdana M, Cemazar M, Pegan K, Mars T - Radiol Oncol (2013)

Bottom Line: The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6.Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

View Article: PubMed Central - PubMed

Affiliation: University of Primorska, Faculty of Health Science, Izola, Slovenia.

ABSTRACT

Background: Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.

Materials and methods: Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death.

Results: Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).

Conclusions: Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

No MeSH data available.


Related in: MedlinePlus

The effect of different doses of ionizing radiation on the HSP 27 and HSP 70 level in myoblasts 24 h after exposure. Representative Western blots for HSP 27 and HSP 70 (A). Relative levels of HSP 27 and HSP 70 shown as % of control level of proteins in myoblasts not exposed to irradiation (B), * p < 0.05 denotes significant difference in level of HSP in exposed myoblasts vs. level in control non-exposed myoblasts.
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f4-rado-47-04-376: The effect of different doses of ionizing radiation on the HSP 27 and HSP 70 level in myoblasts 24 h after exposure. Representative Western blots for HSP 27 and HSP 70 (A). Relative levels of HSP 27 and HSP 70 shown as % of control level of proteins in myoblasts not exposed to irradiation (B), * p < 0.05 denotes significant difference in level of HSP in exposed myoblasts vs. level in control non-exposed myoblasts.

Mentions: The two most prominent stress proteins, HSP 27 and HSP 70, were followed in myoblasts 24-hours after irradiation. Levels of both proteins were increased in myoblasts exposed to irradiation in comparison with levels in control non-exposed myoblasts, although this increase was insignificant in myoblasts exposed to lower doses of irradiation (4 Gy and 6 Gy), while in myoblasts exposed to 8 Gy irradiation, the HSP 70 level was statistically significantly increased (p < 0.05) (Figure. 4).


Effect of ionizing radiation on human skeletal muscle precursor cells.

Jurdana M, Cemazar M, Pegan K, Mars T - Radiol Oncol (2013)

The effect of different doses of ionizing radiation on the HSP 27 and HSP 70 level in myoblasts 24 h after exposure. Representative Western blots for HSP 27 and HSP 70 (A). Relative levels of HSP 27 and HSP 70 shown as % of control level of proteins in myoblasts not exposed to irradiation (B), * p < 0.05 denotes significant difference in level of HSP in exposed myoblasts vs. level in control non-exposed myoblasts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3814283&req=5

f4-rado-47-04-376: The effect of different doses of ionizing radiation on the HSP 27 and HSP 70 level in myoblasts 24 h after exposure. Representative Western blots for HSP 27 and HSP 70 (A). Relative levels of HSP 27 and HSP 70 shown as % of control level of proteins in myoblasts not exposed to irradiation (B), * p < 0.05 denotes significant difference in level of HSP in exposed myoblasts vs. level in control non-exposed myoblasts.
Mentions: The two most prominent stress proteins, HSP 27 and HSP 70, were followed in myoblasts 24-hours after irradiation. Levels of both proteins were increased in myoblasts exposed to irradiation in comparison with levels in control non-exposed myoblasts, although this increase was insignificant in myoblasts exposed to lower doses of irradiation (4 Gy and 6 Gy), while in myoblasts exposed to 8 Gy irradiation, the HSP 70 level was statistically significantly increased (p < 0.05) (Figure. 4).

Bottom Line: The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6.Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

View Article: PubMed Central - PubMed

Affiliation: University of Primorska, Faculty of Health Science, Izola, Slovenia.

ABSTRACT

Background: Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.

Materials and methods: Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death.

Results: Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).

Conclusions: Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

No MeSH data available.


Related in: MedlinePlus