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Effect of ionizing radiation on human skeletal muscle precursor cells.

Jurdana M, Cemazar M, Pegan K, Mars T - Radiol Oncol (2013)

Bottom Line: The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6.Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

View Article: PubMed Central - PubMed

Affiliation: University of Primorska, Faculty of Health Science, Izola, Slovenia.

ABSTRACT

Background: Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.

Materials and methods: Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death.

Results: Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).

Conclusions: Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

No MeSH data available.


Related in: MedlinePlus

The effect of selected therapeutic doses of ionizing radiation (4 Gy, 6 Gy, 8 Gy) on the proliferation of human skeletal myoblasts assessed 72 h after irradiation. Columns and bars represent means ± SD (n= 8). Means are expressed as arbitrary units which are relative units of absorbance measurement at dual wavelengths of 450–540 nm. The control was used as the predetermined reference measurement. Statistically significant differences (p < 0.001) are indicated by ***. Control (CTR) – non-irradiated myoblast; Inhibitor – myoblasts treated with 10 μM AraC, an inhibitor of cell proliferation; 4 Gy – myoblasts irradiated with 4 Gy; 6 Gy - myoblasts irradiated with 6 Gy; 8 Gy – myoblasts irradiated with 8 Gy.
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f1-rado-47-04-376: The effect of selected therapeutic doses of ionizing radiation (4 Gy, 6 Gy, 8 Gy) on the proliferation of human skeletal myoblasts assessed 72 h after irradiation. Columns and bars represent means ± SD (n= 8). Means are expressed as arbitrary units which are relative units of absorbance measurement at dual wavelengths of 450–540 nm. The control was used as the predetermined reference measurement. Statistically significant differences (p < 0.001) are indicated by ***. Control (CTR) – non-irradiated myoblast; Inhibitor – myoblasts treated with 10 μM AraC, an inhibitor of cell proliferation; 4 Gy – myoblasts irradiated with 4 Gy; 6 Gy - myoblasts irradiated with 6 Gy; 8 Gy – myoblasts irradiated with 8 Gy.

Mentions: Therapeutic doses of ionizing radiation used in the experiment effectively inhibited human myoblast proliferation. Irradiation at all doses statistically significantly inhibited myoblast proliferation to the same level (∼ 0.2 of the untreated control) as the inhibitor of DNA synthesis Ara C (Figure 1).


Effect of ionizing radiation on human skeletal muscle precursor cells.

Jurdana M, Cemazar M, Pegan K, Mars T - Radiol Oncol (2013)

The effect of selected therapeutic doses of ionizing radiation (4 Gy, 6 Gy, 8 Gy) on the proliferation of human skeletal myoblasts assessed 72 h after irradiation. Columns and bars represent means ± SD (n= 8). Means are expressed as arbitrary units which are relative units of absorbance measurement at dual wavelengths of 450–540 nm. The control was used as the predetermined reference measurement. Statistically significant differences (p < 0.001) are indicated by ***. Control (CTR) – non-irradiated myoblast; Inhibitor – myoblasts treated with 10 μM AraC, an inhibitor of cell proliferation; 4 Gy – myoblasts irradiated with 4 Gy; 6 Gy - myoblasts irradiated with 6 Gy; 8 Gy – myoblasts irradiated with 8 Gy.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3814283&req=5

f1-rado-47-04-376: The effect of selected therapeutic doses of ionizing radiation (4 Gy, 6 Gy, 8 Gy) on the proliferation of human skeletal myoblasts assessed 72 h after irradiation. Columns and bars represent means ± SD (n= 8). Means are expressed as arbitrary units which are relative units of absorbance measurement at dual wavelengths of 450–540 nm. The control was used as the predetermined reference measurement. Statistically significant differences (p < 0.001) are indicated by ***. Control (CTR) – non-irradiated myoblast; Inhibitor – myoblasts treated with 10 μM AraC, an inhibitor of cell proliferation; 4 Gy – myoblasts irradiated with 4 Gy; 6 Gy - myoblasts irradiated with 6 Gy; 8 Gy – myoblasts irradiated with 8 Gy.
Mentions: Therapeutic doses of ionizing radiation used in the experiment effectively inhibited human myoblast proliferation. Irradiation at all doses statistically significantly inhibited myoblast proliferation to the same level (∼ 0.2 of the untreated control) as the inhibitor of DNA synthesis Ara C (Figure 1).

Bottom Line: The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6.Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

View Article: PubMed Central - PubMed

Affiliation: University of Primorska, Faculty of Health Science, Izola, Slovenia.

ABSTRACT

Background: Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.

Materials and methods: Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death.

Results: Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).

Conclusions: Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.

No MeSH data available.


Related in: MedlinePlus