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Heterogeneity of uroplakin localization in human normal urothelium, papilloma and papillary carcinoma.

Zupancic D, Romih R - Radiol Oncol (2013)

Bottom Line: We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma.Diverse uroplakin expression was found in papilloma and papillary carcinoma.In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cell Biology, Faculty of Medicine, Ljubljana, Slovenia.

ABSTRACT

Background: Uroplakins are differentiation-related membrane proteins of urothelium. We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma. Because of high recurrence rate of these tumours, treated by transurethral resection, we investigated urothelial tumour, resection border and uninvolved urothelium.

Patients and methods: Urinary bladder samples were obtained from tumour free control subjects and patients with papilloma and papillary carcinoma. Immunohistochemical and immunoelectron labelling of uroplakins were performed.

Results: In normal human urothelium with continuous uroplakin-positive superficial cell layer uroplakins were localized to flattened mature fusiform vesicles and apical plasma membrane of umbrella cells. Diverse uroplakin expression was found in papilloma and papillary carcinoma. Three aberrant differentiation stages of urothelial cells, not found in normal urothelium, were recognized in tumours. Diverse uroplakin expression and aberrant differentiation were occasionally found in resection border and in uninvolved urothelium.

Conclusions: We demonstrated here that uroplakin expression and localization in urothelial tumours is altered when compared to normal urothelium. In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation. It is possible that aberrant differentiation stages of urothelial cells in resection border and in uninvolved urothelium contribute to high recurrence rate.

No MeSH data available.


Related in: MedlinePlus

Immunoelectron microscopy of uroplakins in normal urothelium. (A) Two neighbouring superficial umbrella cells (SC), contain numerous mature fusiform vesicles. A part of underlying intermediate cell (IC) is also seen. (B) In umbrella cell uroplakin-positive apical plasma membrane (arrows) and uroplakin-positive mature fusiform vesicles (asterisks) are heavily labelled with colloidal gold particles. (C) Intermediate cell contains uroplakin-positive transporting vesicles (arrows). The basolateral plasma membrane (arrowheads) is uroplakin-negative. N – nucleus, L – lumen. Scale bars: 1 μm.
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f3-rado-47-04-338: Immunoelectron microscopy of uroplakins in normal urothelium. (A) Two neighbouring superficial umbrella cells (SC), contain numerous mature fusiform vesicles. A part of underlying intermediate cell (IC) is also seen. (B) In umbrella cell uroplakin-positive apical plasma membrane (arrows) and uroplakin-positive mature fusiform vesicles (asterisks) are heavily labelled with colloidal gold particles. (C) Intermediate cell contains uroplakin-positive transporting vesicles (arrows). The basolateral plasma membrane (arrowheads) is uroplakin-negative. N – nucleus, L – lumen. Scale bars: 1 μm.

Mentions: Normal urothelium was covered with superficial cells, which contained uroplakin-positive large flattened mature fusiform vesicles and uroplakin-positive apical plasma membrane (Figure 3A, B). These superficial cells were terminally differentiated and they shared characteristics with umbrella cells described in other species.16,18 Intermediate cells of normal urothelium were generally uroplakin-negative, except for some intermediate cells that contained small, round uroplakin-positive transporting vesicles. Their plasma membrane was always uroplakin-negative (Figure 3C), which is also in agreement with observations in other species.18


Heterogeneity of uroplakin localization in human normal urothelium, papilloma and papillary carcinoma.

Zupancic D, Romih R - Radiol Oncol (2013)

Immunoelectron microscopy of uroplakins in normal urothelium. (A) Two neighbouring superficial umbrella cells (SC), contain numerous mature fusiform vesicles. A part of underlying intermediate cell (IC) is also seen. (B) In umbrella cell uroplakin-positive apical plasma membrane (arrows) and uroplakin-positive mature fusiform vesicles (asterisks) are heavily labelled with colloidal gold particles. (C) Intermediate cell contains uroplakin-positive transporting vesicles (arrows). The basolateral plasma membrane (arrowheads) is uroplakin-negative. N – nucleus, L – lumen. Scale bars: 1 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3814278&req=5

f3-rado-47-04-338: Immunoelectron microscopy of uroplakins in normal urothelium. (A) Two neighbouring superficial umbrella cells (SC), contain numerous mature fusiform vesicles. A part of underlying intermediate cell (IC) is also seen. (B) In umbrella cell uroplakin-positive apical plasma membrane (arrows) and uroplakin-positive mature fusiform vesicles (asterisks) are heavily labelled with colloidal gold particles. (C) Intermediate cell contains uroplakin-positive transporting vesicles (arrows). The basolateral plasma membrane (arrowheads) is uroplakin-negative. N – nucleus, L – lumen. Scale bars: 1 μm.
Mentions: Normal urothelium was covered with superficial cells, which contained uroplakin-positive large flattened mature fusiform vesicles and uroplakin-positive apical plasma membrane (Figure 3A, B). These superficial cells were terminally differentiated and they shared characteristics with umbrella cells described in other species.16,18 Intermediate cells of normal urothelium were generally uroplakin-negative, except for some intermediate cells that contained small, round uroplakin-positive transporting vesicles. Their plasma membrane was always uroplakin-negative (Figure 3C), which is also in agreement with observations in other species.18

Bottom Line: We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma.Diverse uroplakin expression was found in papilloma and papillary carcinoma.In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cell Biology, Faculty of Medicine, Ljubljana, Slovenia.

ABSTRACT

Background: Uroplakins are differentiation-related membrane proteins of urothelium. We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma. Because of high recurrence rate of these tumours, treated by transurethral resection, we investigated urothelial tumour, resection border and uninvolved urothelium.

Patients and methods: Urinary bladder samples were obtained from tumour free control subjects and patients with papilloma and papillary carcinoma. Immunohistochemical and immunoelectron labelling of uroplakins were performed.

Results: In normal human urothelium with continuous uroplakin-positive superficial cell layer uroplakins were localized to flattened mature fusiform vesicles and apical plasma membrane of umbrella cells. Diverse uroplakin expression was found in papilloma and papillary carcinoma. Three aberrant differentiation stages of urothelial cells, not found in normal urothelium, were recognized in tumours. Diverse uroplakin expression and aberrant differentiation were occasionally found in resection border and in uninvolved urothelium.

Conclusions: We demonstrated here that uroplakin expression and localization in urothelial tumours is altered when compared to normal urothelium. In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation. It is possible that aberrant differentiation stages of urothelial cells in resection border and in uninvolved urothelium contribute to high recurrence rate.

No MeSH data available.


Related in: MedlinePlus