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Heterogeneity of uroplakin localization in human normal urothelium, papilloma and papillary carcinoma.

Zupancic D, Romih R - Radiol Oncol (2013)

Bottom Line: We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma.Diverse uroplakin expression was found in papilloma and papillary carcinoma.In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cell Biology, Faculty of Medicine, Ljubljana, Slovenia.

ABSTRACT

Background: Uroplakins are differentiation-related membrane proteins of urothelium. We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma. Because of high recurrence rate of these tumours, treated by transurethral resection, we investigated urothelial tumour, resection border and uninvolved urothelium.

Patients and methods: Urinary bladder samples were obtained from tumour free control subjects and patients with papilloma and papillary carcinoma. Immunohistochemical and immunoelectron labelling of uroplakins were performed.

Results: In normal human urothelium with continuous uroplakin-positive superficial cell layer uroplakins were localized to flattened mature fusiform vesicles and apical plasma membrane of umbrella cells. Diverse uroplakin expression was found in papilloma and papillary carcinoma. Three aberrant differentiation stages of urothelial cells, not found in normal urothelium, were recognized in tumours. Diverse uroplakin expression and aberrant differentiation were occasionally found in resection border and in uninvolved urothelium.

Conclusions: We demonstrated here that uroplakin expression and localization in urothelial tumours is altered when compared to normal urothelium. In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation. It is possible that aberrant differentiation stages of urothelial cells in resection border and in uninvolved urothelium contribute to high recurrence rate.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical labelling of uroplakins in normal urothelium and in papiloma (A) Brown reaction products shows that superficial cells (SC) with high uroplakin expression form a continuous layer in normal urothelium from control subjects. Underlying intermediate (IC) and basal cells (BC) are uroplakin-negative. (B) In the urothelium of a patient with papilloma, superficial cells of some papilae are uroplakin-positive (arrows), while others are uroplakin-negative (arrowhead). (C) Individual intermediate urothelial cells of papilae are uroplakin-positive (arrows). L – lumen. Scale bars: 10 μm.
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f1-rado-47-04-338: Immunohistochemical labelling of uroplakins in normal urothelium and in papiloma (A) Brown reaction products shows that superficial cells (SC) with high uroplakin expression form a continuous layer in normal urothelium from control subjects. Underlying intermediate (IC) and basal cells (BC) are uroplakin-negative. (B) In the urothelium of a patient with papilloma, superficial cells of some papilae are uroplakin-positive (arrows), while others are uroplakin-negative (arrowhead). (C) Individual intermediate urothelial cells of papilae are uroplakin-positive (arrows). L – lumen. Scale bars: 10 μm.

Mentions: Consistent with the notion that uroplakins are expressed in terminally differentiated umbrella cells8,9, we considered as normal urothelium only the regions where immunohistochemical labelling of uroplakins was positive in all superficial cells (Figure 1A).


Heterogeneity of uroplakin localization in human normal urothelium, papilloma and papillary carcinoma.

Zupancic D, Romih R - Radiol Oncol (2013)

Immunohistochemical labelling of uroplakins in normal urothelium and in papiloma (A) Brown reaction products shows that superficial cells (SC) with high uroplakin expression form a continuous layer in normal urothelium from control subjects. Underlying intermediate (IC) and basal cells (BC) are uroplakin-negative. (B) In the urothelium of a patient with papilloma, superficial cells of some papilae are uroplakin-positive (arrows), while others are uroplakin-negative (arrowhead). (C) Individual intermediate urothelial cells of papilae are uroplakin-positive (arrows). L – lumen. Scale bars: 10 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3814278&req=5

f1-rado-47-04-338: Immunohistochemical labelling of uroplakins in normal urothelium and in papiloma (A) Brown reaction products shows that superficial cells (SC) with high uroplakin expression form a continuous layer in normal urothelium from control subjects. Underlying intermediate (IC) and basal cells (BC) are uroplakin-negative. (B) In the urothelium of a patient with papilloma, superficial cells of some papilae are uroplakin-positive (arrows), while others are uroplakin-negative (arrowhead). (C) Individual intermediate urothelial cells of papilae are uroplakin-positive (arrows). L – lumen. Scale bars: 10 μm.
Mentions: Consistent with the notion that uroplakins are expressed in terminally differentiated umbrella cells8,9, we considered as normal urothelium only the regions where immunohistochemical labelling of uroplakins was positive in all superficial cells (Figure 1A).

Bottom Line: We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma.Diverse uroplakin expression was found in papilloma and papillary carcinoma.In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Cell Biology, Faculty of Medicine, Ljubljana, Slovenia.

ABSTRACT

Background: Uroplakins are differentiation-related membrane proteins of urothelium. We compared uroplakin expression and ultrastructural localization in human normal urothelium, papilloma and papillary carcinoma. Because of high recurrence rate of these tumours, treated by transurethral resection, we investigated urothelial tumour, resection border and uninvolved urothelium.

Patients and methods: Urinary bladder samples were obtained from tumour free control subjects and patients with papilloma and papillary carcinoma. Immunohistochemical and immunoelectron labelling of uroplakins were performed.

Results: In normal human urothelium with continuous uroplakin-positive superficial cell layer uroplakins were localized to flattened mature fusiform vesicles and apical plasma membrane of umbrella cells. Diverse uroplakin expression was found in papilloma and papillary carcinoma. Three aberrant differentiation stages of urothelial cells, not found in normal urothelium, were recognized in tumours. Diverse uroplakin expression and aberrant differentiation were occasionally found in resection border and in uninvolved urothelium.

Conclusions: We demonstrated here that uroplakin expression and localization in urothelial tumours is altered when compared to normal urothelium. In patients with papilloma and papillary carcinoma immunolabelling of uroplakins at ultrastructural level shows aberrant urothelial differentiation. It is possible that aberrant differentiation stages of urothelial cells in resection border and in uninvolved urothelium contribute to high recurrence rate.

No MeSH data available.


Related in: MedlinePlus