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Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

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Distribution of TFs and receptor-like kinases (RLKs) in the 12,311 genes common to three Brassicaceae species. TFs were assigned by the Database Arabidopsis Transcription Factors (DATF: http://datf.cbi.pku.edu.cn/) for the 12,311 common genes. RLKs were identified and categorized among genes that have GO terms ‘plasma membrane’ within cellular components.
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pct133-F8: Distribution of TFs and receptor-like kinases (RLKs) in the 12,311 genes common to three Brassicaceae species. TFs were assigned by the Database Arabidopsis Transcription Factors (DATF: http://datf.cbi.pku.edu.cn/) for the 12,311 common genes. RLKs were identified and categorized among genes that have GO terms ‘plasma membrane’ within cellular components.

Mentions: The second category of biological process represented in the data set was ‘regulation of transcription’. Based on the Database Arabidopsis Transcription Factors (DATF: http://datf.cbi.pku.edu.cn/), several families of transcription factors (TFs) were represented in the 12,311 common genes, and 754 TF genes were identified (Supplementary Table S8). Among these, six families of zinc finger (C2H2, C3H), PHD, MYB, bHLH and bZIP groups were represented (Fig. 8A). HECATE encodes a basic helix–loop–helix (bHLH) TF which controls the reproductive development of the transmitting tract and floral organ (Gremski et al. 2007). No Transmitting Tract (NTT) encodes a C2H2/C2HC zinc finger TF expressed in the transmitting tract, which is required for development of both the transmitting tract and the funiculus of ovules (Crawford et al. 2007). Other key genes were also identified: HALF FIELD (HAF) (Crawford et al. 2007, Crawford and Yanofsky 2011), Brassinosteroid enhanced expression 1 (BEE1) and BEE3 (Friedrichsen et al. 2002). HAF, BEE1 and BEE3 encode closely related bHLH TFs that act redundantly to specify reproductive tract tissues, where they are expressed in distinct but overlapping patterns (Crawford and Yanofsky 2011). Thus, although little is known about the TF-controlled genetic network in reproductive development, the data sets of TF are expected to be a valuable resource for the study of genetic networks in papilla cell development in Brassicaceae.Fig. 8


Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Distribution of TFs and receptor-like kinases (RLKs) in the 12,311 genes common to three Brassicaceae species. TFs were assigned by the Database Arabidopsis Transcription Factors (DATF: http://datf.cbi.pku.edu.cn/) for the 12,311 common genes. RLKs were identified and categorized among genes that have GO terms ‘plasma membrane’ within cellular components.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814185&req=5

pct133-F8: Distribution of TFs and receptor-like kinases (RLKs) in the 12,311 genes common to three Brassicaceae species. TFs were assigned by the Database Arabidopsis Transcription Factors (DATF: http://datf.cbi.pku.edu.cn/) for the 12,311 common genes. RLKs were identified and categorized among genes that have GO terms ‘plasma membrane’ within cellular components.
Mentions: The second category of biological process represented in the data set was ‘regulation of transcription’. Based on the Database Arabidopsis Transcription Factors (DATF: http://datf.cbi.pku.edu.cn/), several families of transcription factors (TFs) were represented in the 12,311 common genes, and 754 TF genes were identified (Supplementary Table S8). Among these, six families of zinc finger (C2H2, C3H), PHD, MYB, bHLH and bZIP groups were represented (Fig. 8A). HECATE encodes a basic helix–loop–helix (bHLH) TF which controls the reproductive development of the transmitting tract and floral organ (Gremski et al. 2007). No Transmitting Tract (NTT) encodes a C2H2/C2HC zinc finger TF expressed in the transmitting tract, which is required for development of both the transmitting tract and the funiculus of ovules (Crawford et al. 2007). Other key genes were also identified: HALF FIELD (HAF) (Crawford et al. 2007, Crawford and Yanofsky 2011), Brassinosteroid enhanced expression 1 (BEE1) and BEE3 (Friedrichsen et al. 2002). HAF, BEE1 and BEE3 encode closely related bHLH TFs that act redundantly to specify reproductive tract tissues, where they are expressed in distinct but overlapping patterns (Crawford and Yanofsky 2011). Thus, although little is known about the TF-controlled genetic network in reproductive development, the data sets of TF are expected to be a valuable resource for the study of genetic networks in papilla cell development in Brassicaceae.Fig. 8

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

Show MeSH
Related in: MedlinePlus