Limits...
Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

Show MeSH

Related in: MedlinePlus

Classification of papilla-expressed genes by gene ontology, in A. thaliana, A. halleri and B. rapa. The pistil-expressed gene sets were sorted into six functional categories based on a functional classification of the genes, according to the gene ontology (GO) annotations (Tung et al. 2005, Allen et al. 2010): signal transduction, cell wall-related, stress/defense, metabolism, ion transport and transcription.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3814185&req=5

pct133-F6: Classification of papilla-expressed genes by gene ontology, in A. thaliana, A. halleri and B. rapa. The pistil-expressed gene sets were sorted into six functional categories based on a functional classification of the genes, according to the gene ontology (GO) annotations (Tung et al. 2005, Allen et al. 2010): signal transduction, cell wall-related, stress/defense, metabolism, ion transport and transcription.

Mentions: These gene sets were next sorted into six functional categories, taking into consideration several aspects of the biological role, function and physiology of stigmas, i.e. signal transduction, cell wall-related, stress/defense, metabolism, ion transport and transcription: this categorization is based on a functional classification of the pistil-expressed genes according to the GO annotations (Tung et al. 2005, Allen et al. 2010). From the 17,240, 19,260 and 14,099 genes expressed in papilla cells of A. thaliana, A. halleri and B. rapa, 6,468, 7,481 and 5,700 unigenes were classified into six groups according to their annotations and GO terms, respectively. General classification based on GO terms of the papilla-expressed gene data sets revealed that the proportion of categories showed broad similarities among species, in terms of functional classes (Fig. 6; Supplementary Table S6). These results reflect a degree of functional conservation of papilla cells between Arabidopsis and Brassica, and this is consistent with previous results in stigma (Tung et al. 2005, Allen et al. 2010). They also support the theory that mechanisms for stigmatic function are similar in different species, depending on the degree of species differentiation (Allen et al. 2010). A previous study indicated that the expression profiles of stigma-expressed genes in maize were more similar to those of rice than those of Arabidopsis (Swanson et al. 2004, Li et al. 2007, Xiao et al, 2012).Fig. 6


Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Classification of papilla-expressed genes by gene ontology, in A. thaliana, A. halleri and B. rapa. The pistil-expressed gene sets were sorted into six functional categories based on a functional classification of the genes, according to the gene ontology (GO) annotations (Tung et al. 2005, Allen et al. 2010): signal transduction, cell wall-related, stress/defense, metabolism, ion transport and transcription.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814185&req=5

pct133-F6: Classification of papilla-expressed genes by gene ontology, in A. thaliana, A. halleri and B. rapa. The pistil-expressed gene sets were sorted into six functional categories based on a functional classification of the genes, according to the gene ontology (GO) annotations (Tung et al. 2005, Allen et al. 2010): signal transduction, cell wall-related, stress/defense, metabolism, ion transport and transcription.
Mentions: These gene sets were next sorted into six functional categories, taking into consideration several aspects of the biological role, function and physiology of stigmas, i.e. signal transduction, cell wall-related, stress/defense, metabolism, ion transport and transcription: this categorization is based on a functional classification of the pistil-expressed genes according to the GO annotations (Tung et al. 2005, Allen et al. 2010). From the 17,240, 19,260 and 14,099 genes expressed in papilla cells of A. thaliana, A. halleri and B. rapa, 6,468, 7,481 and 5,700 unigenes were classified into six groups according to their annotations and GO terms, respectively. General classification based on GO terms of the papilla-expressed gene data sets revealed that the proportion of categories showed broad similarities among species, in terms of functional classes (Fig. 6; Supplementary Table S6). These results reflect a degree of functional conservation of papilla cells between Arabidopsis and Brassica, and this is consistent with previous results in stigma (Tung et al. 2005, Allen et al. 2010). They also support the theory that mechanisms for stigmatic function are similar in different species, depending on the degree of species differentiation (Allen et al. 2010). A previous study indicated that the expression profiles of stigma-expressed genes in maize were more similar to those of rice than those of Arabidopsis (Swanson et al. 2004, Li et al. 2007, Xiao et al, 2012).Fig. 6

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

Show MeSH
Related in: MedlinePlus