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Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

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Conservation of papilla-expressed genes in Brassicaceae. Venn diagram for the number of genes in papilla cells from A. thaliana, A. halleri and B. rapa.
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pct133-F5: Conservation of papilla-expressed genes in Brassicaceae. Venn diagram for the number of genes in papilla cells from A. thaliana, A. halleri and B. rapa.

Mentions: To reveal the similarities and differences in papilla-expressed genes in Brassicaceae, the data sets from A. thaliana, A. halleri and B. rapa were compared (Fig. 5). Because the genome of Brassica has been triplicated during the course of evolution, every gene has essentially three copies, i.e. one orthologous and two paralogous genes, in the genome. Thus, in the case of B. rapa, 14,099 unigenes were further selected from the 21,026 unigenes by blast analysis with the e-value cut-off of 1 × E−5, to remove paralogous information and to build one-to-one relationships with the A. thaliana genes. It is noteworthy that there were 12,311 expressed genes common to the data sets from all three species, corresponding to 71% of the total expressed genes in A. thaliana, 64% in A. halleri and 87% in B. rapa, indicating that a large proportion of the expressed genes were common to papilla cells across the species boundary in Brassicaceae. Indeed, among the top 100 most highly expressed genes, 82, 82 and 78 genes in A. thaliana, A. halleri and B. rapa, respectively, were common to all three species (Supplementary Tables S1–S3).Fig. 5


Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Conservation of papilla-expressed genes in Brassicaceae. Venn diagram for the number of genes in papilla cells from A. thaliana, A. halleri and B. rapa.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814185&req=5

pct133-F5: Conservation of papilla-expressed genes in Brassicaceae. Venn diagram for the number of genes in papilla cells from A. thaliana, A. halleri and B. rapa.
Mentions: To reveal the similarities and differences in papilla-expressed genes in Brassicaceae, the data sets from A. thaliana, A. halleri and B. rapa were compared (Fig. 5). Because the genome of Brassica has been triplicated during the course of evolution, every gene has essentially three copies, i.e. one orthologous and two paralogous genes, in the genome. Thus, in the case of B. rapa, 14,099 unigenes were further selected from the 21,026 unigenes by blast analysis with the e-value cut-off of 1 × E−5, to remove paralogous information and to build one-to-one relationships with the A. thaliana genes. It is noteworthy that there were 12,311 expressed genes common to the data sets from all three species, corresponding to 71% of the total expressed genes in A. thaliana, 64% in A. halleri and 87% in B. rapa, indicating that a large proportion of the expressed genes were common to papilla cells across the species boundary in Brassicaceae. Indeed, among the top 100 most highly expressed genes, 82, 82 and 78 genes in A. thaliana, A. halleri and B. rapa, respectively, were common to all three species (Supplementary Tables S1–S3).Fig. 5

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

Show MeSH
Related in: MedlinePlus