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Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

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Related in: MedlinePlus

Comparison of transcriptome data sets from papilla cells, stamen, leaf and flower in A. thaliana. (A) Venn diagram for the number of genes expressed in papilla cells, stamen and leaf. Expression data for stamen and leaf are from Schmid et al. (2005). (B). Venn diagram for the number of genes expressed in papilla cells and flowers. Expression data for flowers are from Schmid et al. (2005).
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pct133-F4: Comparison of transcriptome data sets from papilla cells, stamen, leaf and flower in A. thaliana. (A) Venn diagram for the number of genes expressed in papilla cells, stamen and leaf. Expression data for stamen and leaf are from Schmid et al. (2005). (B). Venn diagram for the number of genes expressed in papilla cells and flowers. Expression data for flowers are from Schmid et al. (2005).

Mentions: To characterize the Arabidopsis papilla-specific genes, LM–RNA-seq data from papilla cells were compared with those from microarray analysis of stamen and leaf in A. thaliana (Schmid et al. 2005) (Fig. 4A). Among the 17,240 genes expressed in papilla cells, approximately half (8,538/17,240) were uniformly detected in all tissues examined here, suggesting that these are conserved genes for general cellular function common to all tissues. In contrast, one-third of the genes (5,668/17,240) were papilla specific. Because papilla cells have specialized functions, focusing on pollination, these genes are expected to be involved in the regulation and maintenance of the specific roles of papilla cells.Fig. 4


Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing.

Osaka M, Matsuda T, Sakazono S, Masuko-Suzuki H, Maeda S, Sewaki M, Sone M, Takahashi H, Nakazono M, Iwano M, Takayama S, Shimizu KK, Yano K, Lim YP, Suzuki G, Suwabe K, Watanabe M - Plant Cell Physiol. (2013)

Comparison of transcriptome data sets from papilla cells, stamen, leaf and flower in A. thaliana. (A) Venn diagram for the number of genes expressed in papilla cells, stamen and leaf. Expression data for stamen and leaf are from Schmid et al. (2005). (B). Venn diagram for the number of genes expressed in papilla cells and flowers. Expression data for flowers are from Schmid et al. (2005).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814185&req=5

pct133-F4: Comparison of transcriptome data sets from papilla cells, stamen, leaf and flower in A. thaliana. (A) Venn diagram for the number of genes expressed in papilla cells, stamen and leaf. Expression data for stamen and leaf are from Schmid et al. (2005). (B). Venn diagram for the number of genes expressed in papilla cells and flowers. Expression data for flowers are from Schmid et al. (2005).
Mentions: To characterize the Arabidopsis papilla-specific genes, LM–RNA-seq data from papilla cells were compared with those from microarray analysis of stamen and leaf in A. thaliana (Schmid et al. 2005) (Fig. 4A). Among the 17,240 genes expressed in papilla cells, approximately half (8,538/17,240) were uniformly detected in all tissues examined here, suggesting that these are conserved genes for general cellular function common to all tissues. In contrast, one-third of the genes (5,668/17,240) were papilla specific. Because papilla cells have specialized functions, focusing on pollination, these genes are expected to be involved in the regulation and maintenance of the specific roles of papilla cells.Fig. 4

Bottom Line: Pollination is an early and critical step in plant reproduction, leading to successful fertilization.As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species.These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.

ABSTRACT
Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen-stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

Show MeSH
Related in: MedlinePlus