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Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.


Storage modulus (G′) of the 0.5% PSC solution containing no genipin with increase in temperature. The temperature was maintained at 25°C for 600 s and then increased to (a) 37, (b) 33, or (c) 30°C.
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fig10: Storage modulus (G′) of the 0.5% PSC solution containing no genipin with increase in temperature. The temperature was maintained at 25°C for 600 s and then increased to (a) 37, (b) 33, or (c) 30°C.

Mentions: The temperature dependence of collagen fibril formation without the influence of genipin crosslinking was also evaluated. Figure 10 shows the change in G′ of a 0.5% PSC solution containing no genipin with increase in temperature from 25 to 30, 33, and 37°C. The initial rise in G′ decelerated with the target temperature. At the target temperature of 30°C, only a slight increase in G′ (6 Pa) was observed in 60 min.


Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Storage modulus (G′) of the 0.5% PSC solution containing no genipin with increase in temperature. The temperature was maintained at 25°C for 600 s and then increased to (a) 37, (b) 33, or (c) 30°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3814099&req=5

fig10: Storage modulus (G′) of the 0.5% PSC solution containing no genipin with increase in temperature. The temperature was maintained at 25°C for 600 s and then increased to (a) 37, (b) 33, or (c) 30°C.
Mentions: The temperature dependence of collagen fibril formation without the influence of genipin crosslinking was also evaluated. Figure 10 shows the change in G′ of a 0.5% PSC solution containing no genipin with increase in temperature from 25 to 30, 33, and 37°C. The initial rise in G′ decelerated with the target temperature. At the target temperature of 30°C, only a slight increase in G′ (6 Pa) was observed in 60 min.

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.