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Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.


Storage modulus (G′) of the 0.5% PSC/genipin solution measured by a temperature-responsive gelation test. The temperature was maintained at 25°C for 600 s and then increased to 37°C. Numbers in figures indicate genipin concentrations.
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fig7: Storage modulus (G′) of the 0.5% PSC/genipin solution measured by a temperature-responsive gelation test. The temperature was maintained at 25°C for 600 s and then increased to 37°C. Numbers in figures indicate genipin concentrations.

Mentions: The effect of the genipin concentration on gelation was investigated by temperature-responsive gelation tests for 0.5% and 0.15% PSC solutions. No significant increase in G′ was observed in the intervals at 25°C for all the solutions (Figure 7). The gel points were almost independent of genipin concentrations: all the 0.15% PSC solutions containing 0–2 mM of genipin passed through gel points in the increasing temperature from 25°C to 37°C (Figure 7(a)). As for the 0.5% PSC solutions containing genipin, gel points were achieved in 160–180 s after the temperatures reached 37°C (Figure 7(b)). The rate of increase in G′ was proportional to the increase in the genipin concentration (Figure 7). The genipin concentration dependence was similar in 0.15% and 0.5% PSC solutions, although G′ values of 0.5% PSC gels were much higher than those of 0.15% gels. The effect of the collagen concentration on gelation was also investigated. The rate of increase in G′ accelerated as the PSC concentration increased from 0.15 to 0.5% (Figure 8).


Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Storage modulus (G′) of the 0.5% PSC/genipin solution measured by a temperature-responsive gelation test. The temperature was maintained at 25°C for 600 s and then increased to 37°C. Numbers in figures indicate genipin concentrations.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig7: Storage modulus (G′) of the 0.5% PSC/genipin solution measured by a temperature-responsive gelation test. The temperature was maintained at 25°C for 600 s and then increased to 37°C. Numbers in figures indicate genipin concentrations.
Mentions: The effect of the genipin concentration on gelation was investigated by temperature-responsive gelation tests for 0.5% and 0.15% PSC solutions. No significant increase in G′ was observed in the intervals at 25°C for all the solutions (Figure 7). The gel points were almost independent of genipin concentrations: all the 0.15% PSC solutions containing 0–2 mM of genipin passed through gel points in the increasing temperature from 25°C to 37°C (Figure 7(a)). As for the 0.5% PSC solutions containing genipin, gel points were achieved in 160–180 s after the temperatures reached 37°C (Figure 7(b)). The rate of increase in G′ was proportional to the increase in the genipin concentration (Figure 7). The genipin concentration dependence was similar in 0.15% and 0.5% PSC solutions, although G′ values of 0.5% PSC gels were much higher than those of 0.15% gels. The effect of the collagen concentration on gelation was also investigated. The rate of increase in G′ accelerated as the PSC concentration increased from 0.15 to 0.5% (Figure 8).

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.