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Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.


Results of a fluidity test for the 0.5% ASC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.
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fig3: Results of a fluidity test for the 0.5% ASC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.

Mentions: Fluidity tests were also conducted for 0.15% ASC/genipin solutions (Figure 3). The solution became turbid immediately after the ASC solution was mixed with the genipin solution. The immediate increase in G′′ resulted in the gel points within 60 s (Figures 3(b) and 3(c)). Even when the ASC solution was mixed with PBS containing no genipin, the solution reached a gel point within 60 s (Figure 3(a)). These results indicated that the immediate increases in G′ of 0.15% ASC/genipin solutions were predominantly because of active fibril formation of ASC at neutral pH.


Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Results of a fluidity test for the 0.5% ASC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Results of a fluidity test for the 0.5% ASC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.
Mentions: Fluidity tests were also conducted for 0.15% ASC/genipin solutions (Figure 3). The solution became turbid immediately after the ASC solution was mixed with the genipin solution. The immediate increase in G′′ resulted in the gel points within 60 s (Figures 3(b) and 3(c)). Even when the ASC solution was mixed with PBS containing no genipin, the solution reached a gel point within 60 s (Figure 3(a)). These results indicated that the immediate increases in G′ of 0.15% ASC/genipin solutions were predominantly because of active fibril formation of ASC at neutral pH.

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.