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Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.


Results of a fluidity test for the 0.5% PSC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.
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fig2: Results of a fluidity test for the 0.5% PSC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.

Mentions: Figure 1 shows the results of the fluidity test for the 0.15% PSC/genipin solutions containing various concentrations of genipin (0–2 mM). In the absence of genipin, G′′ was greater than G′ over the entire range of timepoints investigated (Figure 1(a)), exhibiting a viscous nature. Turbidity was not observed, providing evidence that fibril formation of PSC did not occur in 60 min at 25°C. G′ and G′′ showed time-dependent increases in the presence of genipin, where the gel point, defined as the time at which G′ and G′′ were equal, became shorter with the increasing concentration of genipin (Figures 1(a)–1(d)). The reduced gel points indicated that genipin gradually crosslinked collagen monomers. The gel point was determined to be 2190 ± 180 s (mean ± SD; n = 3) at a genipin concentration of 1 mM. The gel points of the 0.5% PSC/genipin solutions were longer than those of the 0.15% PSC/genipin solutions at the same genipin concentrations (Figure 2). The gel point at a genipin concentration of 2 mM was determined to be 2490 ± 270 s (mean ± SD; n = 3). At a genipin concentration of 1 mM, the solution did not reach a gel point within 60 min in triplicate experiments.


Temperature-responsive gelation of type I collagen solutions involving fibril formation and genipin crosslinking as a potential injectable hydrogel.

Yunoki S, Ohyabu Y, Hatayama H - Int J Biomater (2013)

Results of a fluidity test for the 0.5% PSC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3814099&req=5

fig2: Results of a fluidity test for the 0.5% PSC/genipin solutions containing various concentrations of genipin: (a) 0 mM, (b) 0.5 mM, (c) 1 mM, or (d) 2 mM. Temperatures were maintained at 25°C.
Mentions: Figure 1 shows the results of the fluidity test for the 0.15% PSC/genipin solutions containing various concentrations of genipin (0–2 mM). In the absence of genipin, G′′ was greater than G′ over the entire range of timepoints investigated (Figure 1(a)), exhibiting a viscous nature. Turbidity was not observed, providing evidence that fibril formation of PSC did not occur in 60 min at 25°C. G′ and G′′ showed time-dependent increases in the presence of genipin, where the gel point, defined as the time at which G′ and G′′ were equal, became shorter with the increasing concentration of genipin (Figures 1(a)–1(d)). The reduced gel points indicated that genipin gradually crosslinked collagen monomers. The gel point was determined to be 2190 ± 180 s (mean ± SD; n = 3) at a genipin concentration of 1 mM. The gel points of the 0.5% PSC/genipin solutions were longer than those of the 0.15% PSC/genipin solutions at the same genipin concentrations (Figure 2). The gel point at a genipin concentration of 2 mM was determined to be 2490 ± 270 s (mean ± SD; n = 3). At a genipin concentration of 1 mM, the solution did not reach a gel point within 60 min in triplicate experiments.

Bottom Line: The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points.In specific cases PSC would be preferred over ASC as an injectable gel system.The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, 2-4-10 Aomi, Koto-ku, Tokyo 135-0064, Japan.

ABSTRACT
We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0-2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

No MeSH data available.