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The modern primitives: applying new technological approaches to explore the biology of the earliest red blood cells.

Fraser ST - ISRN Hematol (2013)

Bottom Line: The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development.Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive.While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

View Article: PubMed Central - PubMed

Affiliation: Disciplines of Physiology, Anatomy and Histology, Bosch Institute, School of Medical Sciences, University of Sydney, Medical Foundation Building K25, 92-94 Parramatta Road, Camperdown, NSW 2050, Australia.

ABSTRACT
One of the most critical stages in mammalian embryogenesis is the independent production of the embryo's own circulating, functional red blood cells. Correspondingly, erythrocytes are the first cell type to become functionally mature during embryogenesis. Failure to achieve this invariably leads to in utero lethality. The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development. Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive. While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

No MeSH data available.


The cellular composition of the embryonic blood changes profoundly as the embryo matures. Embryonic blood cells at embryonic day E13.5 are mostly primitive nucleated (magenta) Ter-119+ (yellow) erythroblasts (left panel). Three days later, nucleated erythroid cells are very rare and have been replaced by enucleated Ter-119+ definitive erythrocytes (right panel).
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fig3: The cellular composition of the embryonic blood changes profoundly as the embryo matures. Embryonic blood cells at embryonic day E13.5 are mostly primitive nucleated (magenta) Ter-119+ (yellow) erythroblasts (left panel). Three days later, nucleated erythroid cells are very rare and have been replaced by enucleated Ter-119+ definitive erythrocytes (right panel).

Mentions: A comparison of primitive and definitive erythroid cells in situ is shown in Figure 3. Nucleated Ter-119+ cells dominate the circulation at E13.5. However, just three days later, the circulation is primarily composed of smaller, enucleated definitive erythroid cells. How does these two arms of the erythroid system differ and what is responsible for these differences?


The modern primitives: applying new technological approaches to explore the biology of the earliest red blood cells.

Fraser ST - ISRN Hematol (2013)

The cellular composition of the embryonic blood changes profoundly as the embryo matures. Embryonic blood cells at embryonic day E13.5 are mostly primitive nucleated (magenta) Ter-119+ (yellow) erythroblasts (left panel). Three days later, nucleated erythroid cells are very rare and have been replaced by enucleated Ter-119+ definitive erythrocytes (right panel).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814094&req=5

fig3: The cellular composition of the embryonic blood changes profoundly as the embryo matures. Embryonic blood cells at embryonic day E13.5 are mostly primitive nucleated (magenta) Ter-119+ (yellow) erythroblasts (left panel). Three days later, nucleated erythroid cells are very rare and have been replaced by enucleated Ter-119+ definitive erythrocytes (right panel).
Mentions: A comparison of primitive and definitive erythroid cells in situ is shown in Figure 3. Nucleated Ter-119+ cells dominate the circulation at E13.5. However, just three days later, the circulation is primarily composed of smaller, enucleated definitive erythroid cells. How does these two arms of the erythroid system differ and what is responsible for these differences?

Bottom Line: The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development.Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive.While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

View Article: PubMed Central - PubMed

Affiliation: Disciplines of Physiology, Anatomy and Histology, Bosch Institute, School of Medical Sciences, University of Sydney, Medical Foundation Building K25, 92-94 Parramatta Road, Camperdown, NSW 2050, Australia.

ABSTRACT
One of the most critical stages in mammalian embryogenesis is the independent production of the embryo's own circulating, functional red blood cells. Correspondingly, erythrocytes are the first cell type to become functionally mature during embryogenesis. Failure to achieve this invariably leads to in utero lethality. The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development. Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive. While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

No MeSH data available.