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The modern primitives: applying new technological approaches to explore the biology of the earliest red blood cells.

Fraser ST - ISRN Hematol (2013)

Bottom Line: The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development.Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive.While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

View Article: PubMed Central - PubMed

Affiliation: Disciplines of Physiology, Anatomy and Histology, Bosch Institute, School of Medical Sciences, University of Sydney, Medical Foundation Building K25, 92-94 Parramatta Road, Camperdown, NSW 2050, Australia.

ABSTRACT
One of the most critical stages in mammalian embryogenesis is the independent production of the embryo's own circulating, functional red blood cells. Correspondingly, erythrocytes are the first cell type to become functionally mature during embryogenesis. Failure to achieve this invariably leads to in utero lethality. The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development. Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive. While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

No MeSH data available.


Distinct waves of red blood cell production (erythropoiesis) occur throughout ontogeny.
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Related In: Results  -  Collection


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fig1: Distinct waves of red blood cell production (erythropoiesis) occur throughout ontogeny.

Mentions: The independent production of the red cell component of the blood is a critical step in embryonic survival. Embryos will invariably die if they are incapable of forming their own circulatory system, including erythrocytes. The small, enucleated erythrocytes observed in healthy adult circulation are not the first blood cells to be generated. A number of different waves of erythroid cells are formed throughout mammalian embryogenesis (Figure 1). The first wave to appear is the primitive erythroid lineage, which is the major focus of this review. Cells forming this lineage appear shortly after gastrulation, are large, express embryonic globins, and circulate for much of their existence with a nucleus. Progenitors of these cells are found in the YS from E7.5 to E8.5, after which they migrate into the developing circulatory system and dominate the blood stream until the liver becomes a site of hematopoietic progenitor cell expansion and maturation (from E12.5 in the mouse fetus) [7, 8, 30].


The modern primitives: applying new technological approaches to explore the biology of the earliest red blood cells.

Fraser ST - ISRN Hematol (2013)

Distinct waves of red blood cell production (erythropoiesis) occur throughout ontogeny.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814094&req=5

fig1: Distinct waves of red blood cell production (erythropoiesis) occur throughout ontogeny.
Mentions: The independent production of the red cell component of the blood is a critical step in embryonic survival. Embryos will invariably die if they are incapable of forming their own circulatory system, including erythrocytes. The small, enucleated erythrocytes observed in healthy adult circulation are not the first blood cells to be generated. A number of different waves of erythroid cells are formed throughout mammalian embryogenesis (Figure 1). The first wave to appear is the primitive erythroid lineage, which is the major focus of this review. Cells forming this lineage appear shortly after gastrulation, are large, express embryonic globins, and circulate for much of their existence with a nucleus. Progenitors of these cells are found in the YS from E7.5 to E8.5, after which they migrate into the developing circulatory system and dominate the blood stream until the liver becomes a site of hematopoietic progenitor cell expansion and maturation (from E12.5 in the mouse fetus) [7, 8, 30].

Bottom Line: The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development.Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive.While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

View Article: PubMed Central - PubMed

Affiliation: Disciplines of Physiology, Anatomy and Histology, Bosch Institute, School of Medical Sciences, University of Sydney, Medical Foundation Building K25, 92-94 Parramatta Road, Camperdown, NSW 2050, Australia.

ABSTRACT
One of the most critical stages in mammalian embryogenesis is the independent production of the embryo's own circulating, functional red blood cells. Correspondingly, erythrocytes are the first cell type to become functionally mature during embryogenesis. Failure to achieve this invariably leads to in utero lethality. The recent application of technologies such as transcriptome analysis, flow cytometry, mutant embryo analysis, and transgenic fluorescent gene expression reporter systems has shed new light on the distinct erythroid lineages that arise early in development. Here, I will describe the similarities and differences between the distinct erythroid populations that must form for the embryo to survive. While much of the focus of this review will be the poorly understood primitive erythroid lineage, a discussion of other erythroid and hematopoietic lineages, as well as the cell types making up the different niches that give rise to these lineages, is essential for presenting an appropriate developmental context of these cells.

No MeSH data available.