Limits...
Kai-xin-san, a chinese herbal decoction containing ginseng radix et rhizoma, polygalae radix, acori tatarinowii rhizoma, and poria, stimulates the expression and secretion of neurotrophic factors in cultured astrocytes.

Zhu KY, Xu SL, Choi RC, Yan AL, Dong TT, Tsim KW - Evid Based Complement Alternat Med (2013)

Bottom Line: In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction.The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase.The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Center for Chinese Medicine and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong ; Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, China.

ABSTRACT
Kai-xin-san (KXS), a Chinese herbal decoction prescribed by Sun Simiao in Beiji Qianjin Yaofang about 1400 years ago, contains Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria. In China, KXS has been used to treat stress-related psychiatric diseases with the symptoms of depression and forgetfulness. Although animal study has supported the antidepression function of KXS, the mechanism in cellular level is still unknown. Here, a chemically standardized water extract of KXS was applied onto cultured astrocytes in exploring the action mechanisms of KXS treatment, which significantly stimulated the expression and secretion of neurotrophic factors, including NGF, BDNF, and GDNF, in a dose-dependent manner: the stimulation was both in mRNA and protein levels. In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction. The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase. The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

No MeSH data available.


Related in: MedlinePlus

KXS stimulates the mRNA expression of neurotrophic factors in astrocytes. The mRNA expression levels of neurotrophic factors (NGF, GDNF, BDNF, NT3, NT4, and NT5) in astrocytes were analyzed. The astrocytes were treated with different formulations of KXS (0.5–50 μg/mL) for 24 hours. The mRNA was determined by quantitative PCR. Primers for neurotrophic factors were listed in Supplementary Table 1. Available online at http://dx.doi.org/10.1155/2013/731385 Bt2-cAMP (1 mM) was used as a control. Data are expressed as x Basal where the value of control is set as 1 and in Mean ± SEM, n = 4.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3814066&req=5

fig2: KXS stimulates the mRNA expression of neurotrophic factors in astrocytes. The mRNA expression levels of neurotrophic factors (NGF, GDNF, BDNF, NT3, NT4, and NT5) in astrocytes were analyzed. The astrocytes were treated with different formulations of KXS (0.5–50 μg/mL) for 24 hours. The mRNA was determined by quantitative PCR. Primers for neurotrophic factors were listed in Supplementary Table 1. Available online at http://dx.doi.org/10.1155/2013/731385 Bt2-cAMP (1 mM) was used as a control. Data are expressed as x Basal where the value of control is set as 1 and in Mean ± SEM, n = 4.

Mentions: The effect of the three formulations of KXS on the expression of neurotrophic factors was evaluated by quantitative PCR, which included NGF, BDNF, GDNF, NT3, NT4, and NT5. The specific primers of these neurotrophic factors were listed in Supplementary Table 1. Astrocytes cultured at DIV 12 were treated with KXS from 0.5–50 μg/mL for 24 hours, and then the amounts of mRNA were analyzed. The KXS treatment significantly increased the mRNA levels of NGF, BDNF, GDNF, and NT3 in dose-dependent manners (Figure 2). For NGF, the mRNA expression level showed over 2-fold enhancement under the treatment of KXS from 5 to 50 μg/mL, while the DZW-652 showed the best effect (~4-fold) at concentration of 15 μg/mL. For BDNF, DZW-652 increased the mRNA expression level over 2.5-fold. The best effect of DZW-652 could be also observed in GDNF induction. For NT3, K-984 showed better effect (~2-fold). For NT4 and NT5, there were slight changes observed after KXS treatment (Figure 2). Thus, KXS having a high ratio of Ginseng Radix et Rhizoma and Polygalae Radix could benefit the expression of neurotrophic factors, as this was in DZW-652 formulation.


Kai-xin-san, a chinese herbal decoction containing ginseng radix et rhizoma, polygalae radix, acori tatarinowii rhizoma, and poria, stimulates the expression and secretion of neurotrophic factors in cultured astrocytes.

Zhu KY, Xu SL, Choi RC, Yan AL, Dong TT, Tsim KW - Evid Based Complement Alternat Med (2013)

KXS stimulates the mRNA expression of neurotrophic factors in astrocytes. The mRNA expression levels of neurotrophic factors (NGF, GDNF, BDNF, NT3, NT4, and NT5) in astrocytes were analyzed. The astrocytes were treated with different formulations of KXS (0.5–50 μg/mL) for 24 hours. The mRNA was determined by quantitative PCR. Primers for neurotrophic factors were listed in Supplementary Table 1. Available online at http://dx.doi.org/10.1155/2013/731385 Bt2-cAMP (1 mM) was used as a control. Data are expressed as x Basal where the value of control is set as 1 and in Mean ± SEM, n = 4.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814066&req=5

fig2: KXS stimulates the mRNA expression of neurotrophic factors in astrocytes. The mRNA expression levels of neurotrophic factors (NGF, GDNF, BDNF, NT3, NT4, and NT5) in astrocytes were analyzed. The astrocytes were treated with different formulations of KXS (0.5–50 μg/mL) for 24 hours. The mRNA was determined by quantitative PCR. Primers for neurotrophic factors were listed in Supplementary Table 1. Available online at http://dx.doi.org/10.1155/2013/731385 Bt2-cAMP (1 mM) was used as a control. Data are expressed as x Basal where the value of control is set as 1 and in Mean ± SEM, n = 4.
Mentions: The effect of the three formulations of KXS on the expression of neurotrophic factors was evaluated by quantitative PCR, which included NGF, BDNF, GDNF, NT3, NT4, and NT5. The specific primers of these neurotrophic factors were listed in Supplementary Table 1. Astrocytes cultured at DIV 12 were treated with KXS from 0.5–50 μg/mL for 24 hours, and then the amounts of mRNA were analyzed. The KXS treatment significantly increased the mRNA levels of NGF, BDNF, GDNF, and NT3 in dose-dependent manners (Figure 2). For NGF, the mRNA expression level showed over 2-fold enhancement under the treatment of KXS from 5 to 50 μg/mL, while the DZW-652 showed the best effect (~4-fold) at concentration of 15 μg/mL. For BDNF, DZW-652 increased the mRNA expression level over 2.5-fold. The best effect of DZW-652 could be also observed in GDNF induction. For NT3, K-984 showed better effect (~2-fold). For NT4 and NT5, there were slight changes observed after KXS treatment (Figure 2). Thus, KXS having a high ratio of Ginseng Radix et Rhizoma and Polygalae Radix could benefit the expression of neurotrophic factors, as this was in DZW-652 formulation.

Bottom Line: In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction.The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase.The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Center for Chinese Medicine and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong ; Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, China.

ABSTRACT
Kai-xin-san (KXS), a Chinese herbal decoction prescribed by Sun Simiao in Beiji Qianjin Yaofang about 1400 years ago, contains Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria. In China, KXS has been used to treat stress-related psychiatric diseases with the symptoms of depression and forgetfulness. Although animal study has supported the antidepression function of KXS, the mechanism in cellular level is still unknown. Here, a chemically standardized water extract of KXS was applied onto cultured astrocytes in exploring the action mechanisms of KXS treatment, which significantly stimulated the expression and secretion of neurotrophic factors, including NGF, BDNF, and GDNF, in a dose-dependent manner: the stimulation was both in mRNA and protein levels. In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction. The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase. The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

No MeSH data available.


Related in: MedlinePlus