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Kai-xin-san, a chinese herbal decoction containing ginseng radix et rhizoma, polygalae radix, acori tatarinowii rhizoma, and poria, stimulates the expression and secretion of neurotrophic factors in cultured astrocytes.

Zhu KY, Xu SL, Choi RC, Yan AL, Dong TT, Tsim KW - Evid Based Complement Alternat Med (2013)

Bottom Line: In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction.The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase.The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Center for Chinese Medicine and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong ; Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, China.

ABSTRACT
Kai-xin-san (KXS), a Chinese herbal decoction prescribed by Sun Simiao in Beiji Qianjin Yaofang about 1400 years ago, contains Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria. In China, KXS has been used to treat stress-related psychiatric diseases with the symptoms of depression and forgetfulness. Although animal study has supported the antidepression function of KXS, the mechanism in cellular level is still unknown. Here, a chemically standardized water extract of KXS was applied onto cultured astrocytes in exploring the action mechanisms of KXS treatment, which significantly stimulated the expression and secretion of neurotrophic factors, including NGF, BDNF, and GDNF, in a dose-dependent manner: the stimulation was both in mRNA and protein levels. In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction. The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase. The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

No MeSH data available.


Related in: MedlinePlus

Cultures of rat astrocytes. (a) Cultured astrocytes were harvested on DIV4, 8, 12, 16, and 20, and the mRNA encoding GFAP was analyzed. Values are expressed as the percentage of increase to DIV 4 (set as 1) and in mean ± SEM, n = 3. (b) Cultured astrocytes were observed under the microscopy. Meanwhile, cultured astrocytes were stained by Cy3-anti-GFAP (shown in red) and anti-rabbit anti-oligo2 (shown in green) polyclonal antibodies observed under confocal microscopy in the presence of 0.1% Triton-X 100. Bar = 10 μm. Representative figures are showed here, n = 3.
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fig1: Cultures of rat astrocytes. (a) Cultured astrocytes were harvested on DIV4, 8, 12, 16, and 20, and the mRNA encoding GFAP was analyzed. Values are expressed as the percentage of increase to DIV 4 (set as 1) and in mean ± SEM, n = 3. (b) Cultured astrocytes were observed under the microscopy. Meanwhile, cultured astrocytes were stained by Cy3-anti-GFAP (shown in red) and anti-rabbit anti-oligo2 (shown in green) polyclonal antibodies observed under confocal microscopy in the presence of 0.1% Triton-X 100. Bar = 10 μm. Representative figures are showed here, n = 3.

Mentions: Cultured astrocytes reached confluence on the day in vitro (DIV) 12 having the mRNA level of glial fibrillary acidic protein (GFAP), a marker for astrocyte, reaching the peak on DIV 12 to 16 (Figure 1(a)). To test the culture purity, the astrocytes were seeded onto the glass cover slip. At DIV 12, the culture was fixed for immunofluorescent staining with GFAP and possible contamination of oligodendrocytes, which was viewed under the microscopy (Figure 1(b)). The result showed that the majority of cell population was astrocyte, and thus, this stage of astrocyte was used subsequently for all biochemical analyses.


Kai-xin-san, a chinese herbal decoction containing ginseng radix et rhizoma, polygalae radix, acori tatarinowii rhizoma, and poria, stimulates the expression and secretion of neurotrophic factors in cultured astrocytes.

Zhu KY, Xu SL, Choi RC, Yan AL, Dong TT, Tsim KW - Evid Based Complement Alternat Med (2013)

Cultures of rat astrocytes. (a) Cultured astrocytes were harvested on DIV4, 8, 12, 16, and 20, and the mRNA encoding GFAP was analyzed. Values are expressed as the percentage of increase to DIV 4 (set as 1) and in mean ± SEM, n = 3. (b) Cultured astrocytes were observed under the microscopy. Meanwhile, cultured astrocytes were stained by Cy3-anti-GFAP (shown in red) and anti-rabbit anti-oligo2 (shown in green) polyclonal antibodies observed under confocal microscopy in the presence of 0.1% Triton-X 100. Bar = 10 μm. Representative figures are showed here, n = 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814066&req=5

fig1: Cultures of rat astrocytes. (a) Cultured astrocytes were harvested on DIV4, 8, 12, 16, and 20, and the mRNA encoding GFAP was analyzed. Values are expressed as the percentage of increase to DIV 4 (set as 1) and in mean ± SEM, n = 3. (b) Cultured astrocytes were observed under the microscopy. Meanwhile, cultured astrocytes were stained by Cy3-anti-GFAP (shown in red) and anti-rabbit anti-oligo2 (shown in green) polyclonal antibodies observed under confocal microscopy in the presence of 0.1% Triton-X 100. Bar = 10 μm. Representative figures are showed here, n = 3.
Mentions: Cultured astrocytes reached confluence on the day in vitro (DIV) 12 having the mRNA level of glial fibrillary acidic protein (GFAP), a marker for astrocyte, reaching the peak on DIV 12 to 16 (Figure 1(a)). To test the culture purity, the astrocytes were seeded onto the glass cover slip. At DIV 12, the culture was fixed for immunofluorescent staining with GFAP and possible contamination of oligodendrocytes, which was viewed under the microscopy (Figure 1(b)). The result showed that the majority of cell population was astrocyte, and thus, this stage of astrocyte was used subsequently for all biochemical analyses.

Bottom Line: In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction.The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase.The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

View Article: PubMed Central - PubMed

Affiliation: Division of Life Science, Center for Chinese Medicine and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong ; Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, China.

ABSTRACT
Kai-xin-san (KXS), a Chinese herbal decoction prescribed by Sun Simiao in Beiji Qianjin Yaofang about 1400 years ago, contains Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria. In China, KXS has been used to treat stress-related psychiatric diseases with the symptoms of depression and forgetfulness. Although animal study has supported the antidepression function of KXS, the mechanism in cellular level is still unknown. Here, a chemically standardized water extract of KXS was applied onto cultured astrocytes in exploring the action mechanisms of KXS treatment, which significantly stimulated the expression and secretion of neurotrophic factors, including NGF, BDNF, and GDNF, in a dose-dependent manner: the stimulation was both in mRNA and protein levels. In addition, the water extracts of four individual herbs did not significantly stimulate the expression of neurotrophic factors, which could explain the optimized effect of KXS in a herbal decoction. The KXS-induced expression of neurotrophic factors did not depend on signaling mediated by estrogen receptor or protein kinase. The results suggested that the antidepressant-like action of KXS might be mediated by an increase of expression of neurotrophic factors in astrocytes, which fully supported the clinical usage of this decoction.

No MeSH data available.


Related in: MedlinePlus