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Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty.

Hu Z, Zhao G, Wang L, Pu B, Hao J, Lao H, Zhang X, Gan Q, Jiang W - Int J Endocrinol (2013)

Bottom Line: From 4 weeks to 12 weeks after injection of PMMA.The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks.The osteocalcin expression continued to increase during 4 to 12 week.

View Article: PubMed Central - PubMed

Affiliation: Department of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, China.

ABSTRACT
Percutaneous vertebroplasty (PVP) is widely used in the treatment of painful osteoporotic vertebral compression fractures with the injection of PMMA cement, and the controversy for PMMA damage to the osteoporotic bone tissue and to affect the fractures repairing never stops. 72 old female rabbits, each age 3.0~3.5 y, rabbits were assigned randomly to two groups of thirty-six each; PMMA cement were injected into vertebral body in rabbits via mimic PVP, sacrificed at 1 h, 24 h, 3 d, 7 d, 4 w, and 12 w. The expression VEGF and collagen type I, the tissue response, and repair reaction in the interface between PMMA and bone tissue were observed dynamically with RT-PCR and western blot technique; the osteocalcin expression were studied by immunohistochemistry. Compared with the control group, the expression of collagen I increased at 1 hour and was higher from 24 h to 3 d. From 4 weeks to 12 weeks after injection of PMMA. The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks. The osteocalcin expression continued to increase during 4 to 12 week. PMMA would not cause local bone permanent necrosis, and interface injury repairing cycle could be prolonged in a vertebroplasty.

No MeSH data available.


Related in: MedlinePlus

(a) OC expression slightly increased in PMMA-bone interface in 1 week, 20 × 10SP + HE. (b) OC expression in PMMA-bone interface in 4 weeks, when chondrocytes were found to be growing in cluster and differentiating to woven bone, 20 × 10SP + HE.
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Related In: Results  -  Collection


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fig6: (a) OC expression slightly increased in PMMA-bone interface in 1 week, 20 × 10SP + HE. (b) OC expression in PMMA-bone interface in 4 weeks, when chondrocytes were found to be growing in cluster and differentiating to woven bone, 20 × 10SP + HE.

Mentions: Compared with normal bone tissue, after 1 h, 24 h, and 3 d, the OC expression had no significant increase in both groups (P < 0.05). At 7 days, the OC expression of control group increased slightly, the PMMA group had no significant change (P > 0.05). At 4 weeks, the OC expression increased in both groups, but the control group was more significant than the PMMA group (P > 0.05). The OC expression continued to increase during 4 to 12 weeks in experimental group. It is slightly decreased in the control group (P > 0.05) (Figures 6(a) and 6(b)).


Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty.

Hu Z, Zhao G, Wang L, Pu B, Hao J, Lao H, Zhang X, Gan Q, Jiang W - Int J Endocrinol (2013)

(a) OC expression slightly increased in PMMA-bone interface in 1 week, 20 × 10SP + HE. (b) OC expression in PMMA-bone interface in 4 weeks, when chondrocytes were found to be growing in cluster and differentiating to woven bone, 20 × 10SP + HE.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814054&req=5

fig6: (a) OC expression slightly increased in PMMA-bone interface in 1 week, 20 × 10SP + HE. (b) OC expression in PMMA-bone interface in 4 weeks, when chondrocytes were found to be growing in cluster and differentiating to woven bone, 20 × 10SP + HE.
Mentions: Compared with normal bone tissue, after 1 h, 24 h, and 3 d, the OC expression had no significant increase in both groups (P < 0.05). At 7 days, the OC expression of control group increased slightly, the PMMA group had no significant change (P > 0.05). At 4 weeks, the OC expression increased in both groups, but the control group was more significant than the PMMA group (P > 0.05). The OC expression continued to increase during 4 to 12 weeks in experimental group. It is slightly decreased in the control group (P > 0.05) (Figures 6(a) and 6(b)).

Bottom Line: From 4 weeks to 12 weeks after injection of PMMA.The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks.The osteocalcin expression continued to increase during 4 to 12 week.

View Article: PubMed Central - PubMed

Affiliation: Department of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, China.

ABSTRACT
Percutaneous vertebroplasty (PVP) is widely used in the treatment of painful osteoporotic vertebral compression fractures with the injection of PMMA cement, and the controversy for PMMA damage to the osteoporotic bone tissue and to affect the fractures repairing never stops. 72 old female rabbits, each age 3.0~3.5 y, rabbits were assigned randomly to two groups of thirty-six each; PMMA cement were injected into vertebral body in rabbits via mimic PVP, sacrificed at 1 h, 24 h, 3 d, 7 d, 4 w, and 12 w. The expression VEGF and collagen type I, the tissue response, and repair reaction in the interface between PMMA and bone tissue were observed dynamically with RT-PCR and western blot technique; the osteocalcin expression were studied by immunohistochemistry. Compared with the control group, the expression of collagen I increased at 1 hour and was higher from 24 h to 3 d. From 4 weeks to 12 weeks after injection of PMMA. The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks. The osteocalcin expression continued to increase during 4 to 12 week. PMMA would not cause local bone permanent necrosis, and interface injury repairing cycle could be prolonged in a vertebroplasty.

No MeSH data available.


Related in: MedlinePlus