Limits...
Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty.

Hu Z, Zhao G, Wang L, Pu B, Hao J, Lao H, Zhang X, Gan Q, Jiang W - Int J Endocrinol (2013)

Bottom Line: From 4 weeks to 12 weeks after injection of PMMA.The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks.The osteocalcin expression continued to increase during 4 to 12 week.

View Article: PubMed Central - PubMed

Affiliation: Department of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, China.

ABSTRACT
Percutaneous vertebroplasty (PVP) is widely used in the treatment of painful osteoporotic vertebral compression fractures with the injection of PMMA cement, and the controversy for PMMA damage to the osteoporotic bone tissue and to affect the fractures repairing never stops. 72 old female rabbits, each age 3.0~3.5 y, rabbits were assigned randomly to two groups of thirty-six each; PMMA cement were injected into vertebral body in rabbits via mimic PVP, sacrificed at 1 h, 24 h, 3 d, 7 d, 4 w, and 12 w. The expression VEGF and collagen type I, the tissue response, and repair reaction in the interface between PMMA and bone tissue were observed dynamically with RT-PCR and western blot technique; the osteocalcin expression were studied by immunohistochemistry. Compared with the control group, the expression of collagen I increased at 1 hour and was higher from 24 h to 3 d. From 4 weeks to 12 weeks after injection of PMMA. The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks. The osteocalcin expression continued to increase during 4 to 12 week. PMMA would not cause local bone permanent necrosis, and interface injury repairing cycle could be prolonged in a vertebroplasty.

No MeSH data available.


Related in: MedlinePlus

(a) Inflammatory cells infiltration after PMMA injected 3 d, HE ×50. (b) Inflammatory reaction lightened after PMMA injected 7 d, HE ×50.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3814054&req=5

fig2: (a) Inflammatory cells infiltration after PMMA injected 3 d, HE ×50. (b) Inflammatory reaction lightened after PMMA injected 7 d, HE ×50.

Mentions: Compared with that of control, the samples were tightly combined with PMMA cement. The interface between bone tissues and PMMA was infiltrated with inflammatory cells and fibrous tissues. Inflammatory cell infiltration was obvious in 24 h, developed to a peak in 3 d, alleviated comparatively in 7 d. Chondrocytes were found growing in cluster and differentiating to woven bone at the interface without conspicuous inflammatory cells in 4 w; massive lamellar bone formed and occasional haematopoietic bone marrow could be observed without any inflammation in 12 w (Figures 1, 2, and 3).


Related Biological Research in the Interface between Bone Cement and Bone after Percutaneous Vertebroplasty.

Hu Z, Zhao G, Wang L, Pu B, Hao J, Lao H, Zhang X, Gan Q, Jiang W - Int J Endocrinol (2013)

(a) Inflammatory cells infiltration after PMMA injected 3 d, HE ×50. (b) Inflammatory reaction lightened after PMMA injected 7 d, HE ×50.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3814054&req=5

fig2: (a) Inflammatory cells infiltration after PMMA injected 3 d, HE ×50. (b) Inflammatory reaction lightened after PMMA injected 7 d, HE ×50.
Mentions: Compared with that of control, the samples were tightly combined with PMMA cement. The interface between bone tissues and PMMA was infiltrated with inflammatory cells and fibrous tissues. Inflammatory cell infiltration was obvious in 24 h, developed to a peak in 3 d, alleviated comparatively in 7 d. Chondrocytes were found growing in cluster and differentiating to woven bone at the interface without conspicuous inflammatory cells in 4 w; massive lamellar bone formed and occasional haematopoietic bone marrow could be observed without any inflammation in 12 w (Figures 1, 2, and 3).

Bottom Line: From 4 weeks to 12 weeks after injection of PMMA.The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks.The osteocalcin expression continued to increase during 4 to 12 week.

View Article: PubMed Central - PubMed

Affiliation: Department of Spine Surgery, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing 400016, China.

ABSTRACT
Percutaneous vertebroplasty (PVP) is widely used in the treatment of painful osteoporotic vertebral compression fractures with the injection of PMMA cement, and the controversy for PMMA damage to the osteoporotic bone tissue and to affect the fractures repairing never stops. 72 old female rabbits, each age 3.0~3.5 y, rabbits were assigned randomly to two groups of thirty-six each; PMMA cement were injected into vertebral body in rabbits via mimic PVP, sacrificed at 1 h, 24 h, 3 d, 7 d, 4 w, and 12 w. The expression VEGF and collagen type I, the tissue response, and repair reaction in the interface between PMMA and bone tissue were observed dynamically with RT-PCR and western blot technique; the osteocalcin expression were studied by immunohistochemistry. Compared with the control group, the expression of collagen I increased at 1 hour and was higher from 24 h to 3 d. From 4 weeks to 12 weeks after injection of PMMA. The expression of VEGF decreased at 1 hour and 24 hours, significantly increased at 3 days, decreased once again at 7 days, then increased significantly at 4-12 weeks. The osteocalcin expression continued to increase during 4 to 12 week. PMMA would not cause local bone permanent necrosis, and interface injury repairing cycle could be prolonged in a vertebroplasty.

No MeSH data available.


Related in: MedlinePlus