Limits...
DNA damage in rheumatoid arthritis: an age-dependent increase in the lipid peroxidation-derived DNA adduct, heptanone-etheno-2'-deoxycytidine.

Ogawa M, Matsuda T, Ogata A, Hamasaki T, Kumanogoh A, Toyofuku T, Tanaka T - Autoimmune Dis (2013)

Bottom Line: Results.H ε dC levels correlated well with the number of swollen joints (r = 0.57, P < 0.0001) and weakly with the number of tender joints (r = 0.26, P = 0.08) of RA patients, while they did not show a significant association with serological markers such as C-reactive protein and matrix metalloproteinase 3.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, Suita, Osaka 565-0871, Japan.

ABSTRACT
Objective. To evaluate what types of DNA damages are detected in rheumatoid arthritis (RA). Methods. The DNA adducts such as 8-oxo-hydroxy-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG), 1,N(6)-etheno-2'-deoxyadenosine ( ε dA), and heptanone-etheno-2'-deoxycytidine (H ε dC) in genomic DNAs, derived from whole blood cells from 46 RA patients and 31 healthy controls, were analyzed by high-performance liquid chromatography tandem mass spectrometry, and their levels in RA patients and controls were compared. In addition, correlation between DNA adducts and clinical parameters of RA was analyzed. Results. Compared with controls, the levels of H ε dC in RA were significantly higher (P < 0.0001) and age dependent (r = 0.43, P < 0.01), while there was no significant difference in 8-oxo-dG and ε dA accumulation between RA patients and controls. H ε dC levels correlated well with the number of swollen joints (r = 0.57, P < 0.0001) and weakly with the number of tender joints (r = 0.26, P = 0.08) of RA patients, while they did not show a significant association with serological markers such as C-reactive protein and matrix metalloproteinase 3. Conclusion. These findings indicate that H ε dC may have some influence on the development of RA and/or its complications.

No MeSH data available.


Related in: MedlinePlus

HεdC levels show positive correlation with the number of swollen joints and the total number of swollen plus tender joints. HεdC levels correlated weakly with the number of tender joints (P = 0.08) but strongly with that of swollen joints (P < 0.0001) and the total number of tender + swollen joints (P = 0.0005). The straight line represents linear approximation of the values of HεdC versus the number of involved joints in RA patients.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3814043&req=5

fig4: HεdC levels show positive correlation with the number of swollen joints and the total number of swollen plus tender joints. HεdC levels correlated weakly with the number of tender joints (P = 0.08) but strongly with that of swollen joints (P < 0.0001) and the total number of tender + swollen joints (P = 0.0005). The straight line represents linear approximation of the values of HεdC versus the number of involved joints in RA patients.

Mentions: Correlation analyses were performed to explore whether HεdC levels correlated with clinical or laboratory test findings including platelet count, concentrations of CRP, serum amyloid A (SAA), albumin, and matrix metalloproteinase 3 (MMP3), as well as levels of total cholesterol, triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in addition to the number of swollen joints and tender joints. The results are shown in Table 2. Slightly positive, but not statistically significant, correlations were observed between the levels of HεdC and the number of platelets, concentrations of CRP, SAA, and MMP3, and the number of tender joints. On the other hand, HεdC levels showed a significantly positive correlation with the number of swollen joints (r = 0.57, P < 0.0001) and the total number of swollen and tender joints (r = 0.48, P = 0.0005) (Table 2 and Figure 4) and were also age-dependent (r = 0.43, P = 0.003) (Table 2 and Figure 3(b)). Finally, HεdC levels showed no associations with disease duration, class and stage, positivity of autoantibodies such as rheumatoid factor and anticitrullinated protein antibody, or use of NSAID, methotrexate, or prednisolone.


DNA damage in rheumatoid arthritis: an age-dependent increase in the lipid peroxidation-derived DNA adduct, heptanone-etheno-2'-deoxycytidine.

Ogawa M, Matsuda T, Ogata A, Hamasaki T, Kumanogoh A, Toyofuku T, Tanaka T - Autoimmune Dis (2013)

HεdC levels show positive correlation with the number of swollen joints and the total number of swollen plus tender joints. HεdC levels correlated weakly with the number of tender joints (P = 0.08) but strongly with that of swollen joints (P < 0.0001) and the total number of tender + swollen joints (P = 0.0005). The straight line represents linear approximation of the values of HεdC versus the number of involved joints in RA patients.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3814043&req=5

fig4: HεdC levels show positive correlation with the number of swollen joints and the total number of swollen plus tender joints. HεdC levels correlated weakly with the number of tender joints (P = 0.08) but strongly with that of swollen joints (P < 0.0001) and the total number of tender + swollen joints (P = 0.0005). The straight line represents linear approximation of the values of HεdC versus the number of involved joints in RA patients.
Mentions: Correlation analyses were performed to explore whether HεdC levels correlated with clinical or laboratory test findings including platelet count, concentrations of CRP, serum amyloid A (SAA), albumin, and matrix metalloproteinase 3 (MMP3), as well as levels of total cholesterol, triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in addition to the number of swollen joints and tender joints. The results are shown in Table 2. Slightly positive, but not statistically significant, correlations were observed between the levels of HεdC and the number of platelets, concentrations of CRP, SAA, and MMP3, and the number of tender joints. On the other hand, HεdC levels showed a significantly positive correlation with the number of swollen joints (r = 0.57, P < 0.0001) and the total number of swollen and tender joints (r = 0.48, P = 0.0005) (Table 2 and Figure 4) and were also age-dependent (r = 0.43, P = 0.003) (Table 2 and Figure 3(b)). Finally, HεdC levels showed no associations with disease duration, class and stage, positivity of autoantibodies such as rheumatoid factor and anticitrullinated protein antibody, or use of NSAID, methotrexate, or prednisolone.

Bottom Line: Results.H ε dC levels correlated well with the number of swollen joints (r = 0.57, P < 0.0001) and weakly with the number of tender joints (r = 0.26, P = 0.08) of RA patients, while they did not show a significant association with serological markers such as C-reactive protein and matrix metalloproteinase 3.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamada-Oka, Suita, Osaka 565-0871, Japan.

ABSTRACT
Objective. To evaluate what types of DNA damages are detected in rheumatoid arthritis (RA). Methods. The DNA adducts such as 8-oxo-hydroxy-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG), 1,N(6)-etheno-2'-deoxyadenosine ( ε dA), and heptanone-etheno-2'-deoxycytidine (H ε dC) in genomic DNAs, derived from whole blood cells from 46 RA patients and 31 healthy controls, were analyzed by high-performance liquid chromatography tandem mass spectrometry, and their levels in RA patients and controls were compared. In addition, correlation between DNA adducts and clinical parameters of RA was analyzed. Results. Compared with controls, the levels of H ε dC in RA were significantly higher (P < 0.0001) and age dependent (r = 0.43, P < 0.01), while there was no significant difference in 8-oxo-dG and ε dA accumulation between RA patients and controls. H ε dC levels correlated well with the number of swollen joints (r = 0.57, P < 0.0001) and weakly with the number of tender joints (r = 0.26, P = 0.08) of RA patients, while they did not show a significant association with serological markers such as C-reactive protein and matrix metalloproteinase 3. Conclusion. These findings indicate that H ε dC may have some influence on the development of RA and/or its complications.

No MeSH data available.


Related in: MedlinePlus