Endothelial reconstitution by CD34+ progenitors derived from baboon embryonic stem cells.
Bottom Line: The efficiency of generating CD34+ EPCs did not differ significantly from ECGS to EGM-2 culture media, however, angioblasts specified in ECGS medium expressed a higher percentage of CD34+/CXCR4+ cells (3.49 ± 1.32%, n = 3) than those specified in EGM-2 medium (0.49 ± 0.52%, n = 3).After 14 days of ex vivo culture, the grafted cells had attached and integrated to the denuded surface; in addition, they had matured further and expressed terminally differentiated endothelial markers including CD31 and CD146.In conclusion, we have proved that specified CD34+ EPCs are promising therapeutic agents for repairing damaged vasculature.
Affiliation: Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX 78245-0549, USA. email@example.comShow MeSH
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Mentions: As demonstrated previously, our angioblast differentiation protocol can generate angioblasts via mesodermal intermediates and the cells demonstrate dual potential to differentiate into haematopoietic and vascular lineages . To direct angioblast differentiation towards functional EPCs, we tested whether endothelial growth media for mature endothelial cultures could specify and drive the angioblasts into EPCs more efficiently. In this study, we chose two media, EGM-2 and ECGS, and continued to culture angioblasts in ADM as a reference control. EGM-2 contains defined growth factors, whereas ECGS contains bovine pituitary extracts. After angioblast differentiation, we transferred EBs onto collagen IV-coated plates and cultured them for 12 days. Figure 2 illustrates the dynamic process of angioblast specifications.
Affiliation: Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX 78245-0549, USA. firstname.lastname@example.org