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Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity.

Jahangir T, Safhi MM, Sultana S, Ahmad S - Interdiscip Toxicol (2013)

Bottom Line: Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses.HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy.In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Jazan University, Saudi Arabia.

ABSTRACT
Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Benzo(a)pyrene (B(a)P) administration leads to depletion of renal glutathione and its metabolizing enzymes. Pretreatment with PL (100 and 200 mg /kg b.wt) restored renal glutathione content and its dependent enzymes significantly (p<0.001) with simultaneous increase in catalase(CAT), quinone reductase(QR) in mouse kidney. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. PL extract pretreated groups showed marked inhibition in B(a)P induced micronuclei formation in mouse bone marrow cells with simultaneous restoration of DNA integrity, viz. alkaline unwinding assay and DNA damage shown by gel-electrophoresis. HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy. In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory.

No MeSH data available.


Related in: MedlinePlus

Effect of pretreatment of PL on B(a)P induced DNA damage in mouse (Alkaline unwinding assay). F value = (auDNA – ssDNA)/(dsDNA – ssDNA).
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Figure 0002: Effect of pretreatment of PL on B(a)P induced DNA damage in mouse (Alkaline unwinding assay). F value = (auDNA – ssDNA)/(dsDNA – ssDNA).

Mentions: Table 4 illustrates the effect of pretreatment of the extract of PL on B(a)P induced micronuclei formation in mouse bone marrow cells where PL extract showed marked inhibition in micronuclei formation at both doses. Figure 1 shows agarose gel electrophoresis where results indicate that there was significant DNA fragmentation only in the B(a)P group as compared to control group and concurrent less fragmentation in PL pretreated groups at both doses. A simultaneous decrease in F-value was also noted in DNA alkaline unwinding assay as evident from Figure 2, which is a marker for alteration in DNA integrity. The F-value was inversely proportional to the number of strand breaks present, and thus has been used as an indicator of DNA integrity. During DNA fragmentation, DNA damage is estimated in terms of smearing and lack of intact bands. Control and only PL treated groups showed less smearing and an intact band. Figure 3 shows an HPTLC plate pictograph confirming the presence of quercetin in PL extract.


Pluchea lanceolata protects against Benzo(a) pyrene induced renal toxicity and loss of DNA integrity.

Jahangir T, Safhi MM, Sultana S, Ahmad S - Interdiscip Toxicol (2013)

Effect of pretreatment of PL on B(a)P induced DNA damage in mouse (Alkaline unwinding assay). F value = (auDNA – ssDNA)/(dsDNA – ssDNA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3795321&req=5

Figure 0002: Effect of pretreatment of PL on B(a)P induced DNA damage in mouse (Alkaline unwinding assay). F value = (auDNA – ssDNA)/(dsDNA – ssDNA).
Mentions: Table 4 illustrates the effect of pretreatment of the extract of PL on B(a)P induced micronuclei formation in mouse bone marrow cells where PL extract showed marked inhibition in micronuclei formation at both doses. Figure 1 shows agarose gel electrophoresis where results indicate that there was significant DNA fragmentation only in the B(a)P group as compared to control group and concurrent less fragmentation in PL pretreated groups at both doses. A simultaneous decrease in F-value was also noted in DNA alkaline unwinding assay as evident from Figure 2, which is a marker for alteration in DNA integrity. The F-value was inversely proportional to the number of strand breaks present, and thus has been used as an indicator of DNA integrity. During DNA fragmentation, DNA damage is estimated in terms of smearing and lack of intact bands. Control and only PL treated groups showed less smearing and an intact band. Figure 3 shows an HPTLC plate pictograph confirming the presence of quercetin in PL extract.

Bottom Line: Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses.HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy.In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, College of Pharmacy, Jazan University, Saudi Arabia.

ABSTRACT
Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Benzo(a)pyrene (B(a)P) administration leads to depletion of renal glutathione and its metabolizing enzymes. Pretreatment with PL (100 and 200 mg /kg b.wt) restored renal glutathione content and its dependent enzymes significantly (p<0.001) with simultaneous increase in catalase(CAT), quinone reductase(QR) in mouse kidney. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. PL extract pretreated groups showed marked inhibition in B(a)P induced micronuclei formation in mouse bone marrow cells with simultaneous restoration of DNA integrity, viz. alkaline unwinding assay and DNA damage shown by gel-electrophoresis. HPTLC confirms the presence of quercetin in plant extract which could be responsible for PL protecting efficacy. In conclusion, the present findings strongly support the antioxidant efficacy of PL, possibly by modulation of antioxidant armory.

No MeSH data available.


Related in: MedlinePlus