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Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection.

Chen Z, Chen J, Wu L, Li W, Chen J, Cheng H, Pan J, Cai B - Int J Nanomedicine (2013)

Bottom Line: HA-BSANPs were successfully prepared with HA coating the surface and amorphous drug in the core.The HA-BSANPs could reside in the articular cavity of rats for more than 14 days, which was significantly longer than BSANPs.HA-BSANPs are a promising carrier for articular-related diseases due to elongated articular residence and improved chondrocytic accumulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.

ABSTRACT

Objective: To evaluate the potential of hyaluronic acid (HA)-coated bovine serum albumin nanoparticles (BSANPs) as a novel chondrocyte-targeting drug-delivery nanomedicine.

Methods: The HA-BSANPs were characterized by dynamic light scattering, transmission electron microscopy, differential scanning calorimetry, and X-ray diffraction. Fluorescence imaging was used to visualize the distribution of nanoparticles after intra-articular injection. The chondrocyte-targeting efficiency and cellular uptake mechanism of HA-BSANPs were investigated using endocytic inhibitors.

Results: HA-BSANPs were successfully prepared with HA coating the surface and amorphous drug in the core. Compared with BSANPs, HA-BSANPs exhibited improved uptake by chondrocytes through a receptor-mediated active uptake mechanism. The endocytosis process of BSANPs and HA-BSANPs involved clathrin-mediated endocytosis, caveolae-mediated endocytosis, and macropinocytosis. No apparent thickening or hyperplasia of the synovium was observed in either BSANPs or HA-BSANPs. The HA-BSANPs could reside in the articular cavity of rats for more than 14 days, which was significantly longer than BSANPs.

Conclusion: HA-BSANPs are a promising carrier for articular-related diseases due to elongated articular residence and improved chondrocytic accumulation.

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Related in: MedlinePlus

The effect of temperature on the in vitro cell uptake of nanoparticles by chondrocyte.Note: **P<0.01, the Q of 4 °C compared with that of 37 °C.Abbreviations:Q, uptake of the unit cell value; BSANPs, bovine serum albumin nanoparticles; HA-BSANPs, hyaluronic acid-coated BSANPs.
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f7-ijn-8-3843: The effect of temperature on the in vitro cell uptake of nanoparticles by chondrocyte.Note: **P<0.01, the Q of 4 °C compared with that of 37 °C.Abbreviations:Q, uptake of the unit cell value; BSANPs, bovine serum albumin nanoparticles; HA-BSANPs, hyaluronic acid-coated BSANPs.

Mentions: The temperature was found to have a more significant effect on the uptake of NPs than brucine solution by chondrocytes (Figure 7). Upon lowering the incubation temperature to 4°C, the uptake of brucine solution, br-BSANPs, and HA-br-BSANPs was reduced by 12.31%, 53.28%, and 69.87%, respectively. The results of this low-temperature assay indicate that both br-BSANPs and HA-br-BSANPs appear to be taken up by the cells via an energy-dependent active process, while free brucine entered the cell mainly through passive diffusion.


Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection.

Chen Z, Chen J, Wu L, Li W, Chen J, Cheng H, Pan J, Cai B - Int J Nanomedicine (2013)

The effect of temperature on the in vitro cell uptake of nanoparticles by chondrocyte.Note: **P<0.01, the Q of 4 °C compared with that of 37 °C.Abbreviations:Q, uptake of the unit cell value; BSANPs, bovine serum albumin nanoparticles; HA-BSANPs, hyaluronic acid-coated BSANPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3794990&req=5

f7-ijn-8-3843: The effect of temperature on the in vitro cell uptake of nanoparticles by chondrocyte.Note: **P<0.01, the Q of 4 °C compared with that of 37 °C.Abbreviations:Q, uptake of the unit cell value; BSANPs, bovine serum albumin nanoparticles; HA-BSANPs, hyaluronic acid-coated BSANPs.
Mentions: The temperature was found to have a more significant effect on the uptake of NPs than brucine solution by chondrocytes (Figure 7). Upon lowering the incubation temperature to 4°C, the uptake of brucine solution, br-BSANPs, and HA-br-BSANPs was reduced by 12.31%, 53.28%, and 69.87%, respectively. The results of this low-temperature assay indicate that both br-BSANPs and HA-br-BSANPs appear to be taken up by the cells via an energy-dependent active process, while free brucine entered the cell mainly through passive diffusion.

Bottom Line: HA-BSANPs were successfully prepared with HA coating the surface and amorphous drug in the core.The HA-BSANPs could reside in the articular cavity of rats for more than 14 days, which was significantly longer than BSANPs.HA-BSANPs are a promising carrier for articular-related diseases due to elongated articular residence and improved chondrocytic accumulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.

ABSTRACT

Objective: To evaluate the potential of hyaluronic acid (HA)-coated bovine serum albumin nanoparticles (BSANPs) as a novel chondrocyte-targeting drug-delivery nanomedicine.

Methods: The HA-BSANPs were characterized by dynamic light scattering, transmission electron microscopy, differential scanning calorimetry, and X-ray diffraction. Fluorescence imaging was used to visualize the distribution of nanoparticles after intra-articular injection. The chondrocyte-targeting efficiency and cellular uptake mechanism of HA-BSANPs were investigated using endocytic inhibitors.

Results: HA-BSANPs were successfully prepared with HA coating the surface and amorphous drug in the core. Compared with BSANPs, HA-BSANPs exhibited improved uptake by chondrocytes through a receptor-mediated active uptake mechanism. The endocytosis process of BSANPs and HA-BSANPs involved clathrin-mediated endocytosis, caveolae-mediated endocytosis, and macropinocytosis. No apparent thickening or hyperplasia of the synovium was observed in either BSANPs or HA-BSANPs. The HA-BSANPs could reside in the articular cavity of rats for more than 14 days, which was significantly longer than BSANPs.

Conclusion: HA-BSANPs are a promising carrier for articular-related diseases due to elongated articular residence and improved chondrocytic accumulation.

Show MeSH
Related in: MedlinePlus