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Functional and molecular changes of the bladder in rats with crushing injury of nerve bundles from major pelvic ganglion to the bladder: role of RhoA/Rho kinase pathway.

Kim SJ, Lee DS, Bae WJ, Kim S, Hong SH, Lee JY, Hwang TK, Kim SW - Int J Mol Sci (2013)

Bottom Line: However, M3 muscarinic receptor expression was not significantly changed.The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury.From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Seoul St. Mary's Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea. hygeia@catholic.ac.kr

ABSTRACT
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

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Related in: MedlinePlus

Anatomic appearance of nerve bundles from major pelvic ganglion (MPG) to the bladder. The nerve bundles were isolated and a crush injury induced using a hemostat clamp for 2 min in the crushing injury on nerve bundles from the MPG to the bladder group.
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f6-ijms-14-17511: Anatomic appearance of nerve bundles from major pelvic ganglion (MPG) to the bladder. The nerve bundles were isolated and a crush injury induced using a hemostat clamp for 2 min in the crushing injury on nerve bundles from the MPG to the bladder group.

Mentions: Tiletamine (Zoletil) 0.2 mL was injected intraperitoneally to anesthetize the animals. A lower midline incision was made and the prostate gland was exposed. After identifying of MPG on the lateral side to bilateral prostates [20], the nerve bundles from the MPG to the bladder were identified and isolated (Figure 6). In sham-operated group, there was no further surgical manipulation. In the crushing injury on nerve bundles from the MPG to the bladder group, the nerve bundles were isolated and a crush injury induced using a hemostat clamp for 2 min and the abdomen was closed. Functional and molecular evaluation of the bladder were performed in control, sham-operated, and crushing injury on nerve bundles from the MPG group after 1, 2, and 4 weeks.


Functional and molecular changes of the bladder in rats with crushing injury of nerve bundles from major pelvic ganglion to the bladder: role of RhoA/Rho kinase pathway.

Kim SJ, Lee DS, Bae WJ, Kim S, Hong SH, Lee JY, Hwang TK, Kim SW - Int J Mol Sci (2013)

Anatomic appearance of nerve bundles from major pelvic ganglion (MPG) to the bladder. The nerve bundles were isolated and a crush injury induced using a hemostat clamp for 2 min in the crushing injury on nerve bundles from the MPG to the bladder group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3794738&req=5

f6-ijms-14-17511: Anatomic appearance of nerve bundles from major pelvic ganglion (MPG) to the bladder. The nerve bundles were isolated and a crush injury induced using a hemostat clamp for 2 min in the crushing injury on nerve bundles from the MPG to the bladder group.
Mentions: Tiletamine (Zoletil) 0.2 mL was injected intraperitoneally to anesthetize the animals. A lower midline incision was made and the prostate gland was exposed. After identifying of MPG on the lateral side to bilateral prostates [20], the nerve bundles from the MPG to the bladder were identified and isolated (Figure 6). In sham-operated group, there was no further surgical manipulation. In the crushing injury on nerve bundles from the MPG to the bladder group, the nerve bundles were isolated and a crush injury induced using a hemostat clamp for 2 min and the abdomen was closed. Functional and molecular evaluation of the bladder were performed in control, sham-operated, and crushing injury on nerve bundles from the MPG group after 1, 2, and 4 weeks.

Bottom Line: However, M3 muscarinic receptor expression was not significantly changed.The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury.From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Seoul St. Mary's Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea. hygeia@catholic.ac.kr

ABSTRACT
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

Show MeSH
Related in: MedlinePlus