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Functional and molecular changes of the bladder in rats with crushing injury of nerve bundles from major pelvic ganglion to the bladder: role of RhoA/Rho kinase pathway.

Kim SJ, Lee DS, Bae WJ, Kim S, Hong SH, Lee JY, Hwang TK, Kim SW - Int J Mol Sci (2013)

Bottom Line: However, M3 muscarinic receptor expression was not significantly changed.The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury.From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Seoul St. Mary's Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea. hygeia@catholic.ac.kr

ABSTRACT
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

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Related in: MedlinePlus

Contractile response to electrical field stimulation in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder group at one (A); two (B); and four (C) weeks after injury. The number of rats analyzed at each of one, two, and four weeks after injury was five in the control group, five in the sham-operated group, and 10 in the crushing injury on nerve bundles from MPG group. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group, *** p < 0.01 compared with the control and sham-operated groups.
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f2-ijms-14-17511: Contractile response to electrical field stimulation in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder group at one (A); two (B); and four (C) weeks after injury. The number of rats analyzed at each of one, two, and four weeks after injury was five in the control group, five in the sham-operated group, and 10 in the crushing injury on nerve bundles from MPG group. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group, *** p < 0.01 compared with the control and sham-operated groups.

Mentions: The results of EFS of bladder strips were shown in Figure 2. At one week after crushing nerve injury, the contractile response was significantly decreased compared with the control and sham-operated groups at each of the four frequencies (2, 4, 8, and 32 Hz) (p < 0.05) (Figure 2A). In addition, the significantly decrease contractile response at two and four weeks after injury was observed compared with the control and sham-operated groups (Figure 2B,C).


Functional and molecular changes of the bladder in rats with crushing injury of nerve bundles from major pelvic ganglion to the bladder: role of RhoA/Rho kinase pathway.

Kim SJ, Lee DS, Bae WJ, Kim S, Hong SH, Lee JY, Hwang TK, Kim SW - Int J Mol Sci (2013)

Contractile response to electrical field stimulation in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder group at one (A); two (B); and four (C) weeks after injury. The number of rats analyzed at each of one, two, and four weeks after injury was five in the control group, five in the sham-operated group, and 10 in the crushing injury on nerve bundles from MPG group. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group, *** p < 0.01 compared with the control and sham-operated groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3794738&req=5

f2-ijms-14-17511: Contractile response to electrical field stimulation in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder group at one (A); two (B); and four (C) weeks after injury. The number of rats analyzed at each of one, two, and four weeks after injury was five in the control group, five in the sham-operated group, and 10 in the crushing injury on nerve bundles from MPG group. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group, *** p < 0.01 compared with the control and sham-operated groups.
Mentions: The results of EFS of bladder strips were shown in Figure 2. At one week after crushing nerve injury, the contractile response was significantly decreased compared with the control and sham-operated groups at each of the four frequencies (2, 4, 8, and 32 Hz) (p < 0.05) (Figure 2A). In addition, the significantly decrease contractile response at two and four weeks after injury was observed compared with the control and sham-operated groups (Figure 2B,C).

Bottom Line: However, M3 muscarinic receptor expression was not significantly changed.The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury.From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Seoul St. Mary's Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea. hygeia@catholic.ac.kr

ABSTRACT
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

Show MeSH
Related in: MedlinePlus