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Functional and molecular changes of the bladder in rats with crushing injury of nerve bundles from major pelvic ganglion to the bladder: role of RhoA/Rho kinase pathway.

Kim SJ, Lee DS, Bae WJ, Kim S, Hong SH, Lee JY, Hwang TK, Kim SW - Int J Mol Sci (2013)

Bottom Line: However, M3 muscarinic receptor expression was not significantly changed.The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury.From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Seoul St. Mary's Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea. hygeia@catholic.ac.kr

ABSTRACT
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

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Related in: MedlinePlus

Bladder weights in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder groups at one, two, and four weeks after injury. Measurement of bladder weights was done in control (n = 5), sham-operated (n = 5), and crushing injury on nerve bundles from MPG (n = 10) groups at each of one, two, and four weeks after injury. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group.
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f1-ijms-14-17511: Bladder weights in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder groups at one, two, and four weeks after injury. Measurement of bladder weights was done in control (n = 5), sham-operated (n = 5), and crushing injury on nerve bundles from MPG (n = 10) groups at each of one, two, and four weeks after injury. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group.

Mentions: At one week after injury, the bladder weight of crushing injury on nerve bundles from the MPG group (740.9 ± 31.6 mg) was significantly increased compared with the control and sham-operated groups (193.4 ± 31.7 and 232.8 ± 55.2 mg, respectively) (p < 0.05). The bladder weight of crushing injury on nerve bundles from the MPG group (738.2 ± 36.2 mg) was significantly increased compared with the control and sham-operated groups (183.2 ± 42.5 and 193.4 ± 35.2 mg, respectively) at two weeks after injury (p < 0.05). Similar with one and two weeks after injury, the bladder weight of crushing injury on nerve bundles from the MPG group (711.5 ± 60.4 mg) was significantly increased compared with the control and sham-operated groups (160.9 ± 44.8 and 197.8 ± 48.2 mg, respectively) (p < 0.05) (Figure 1).


Functional and molecular changes of the bladder in rats with crushing injury of nerve bundles from major pelvic ganglion to the bladder: role of RhoA/Rho kinase pathway.

Kim SJ, Lee DS, Bae WJ, Kim S, Hong SH, Lee JY, Hwang TK, Kim SW - Int J Mol Sci (2013)

Bladder weights in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder groups at one, two, and four weeks after injury. Measurement of bladder weights was done in control (n = 5), sham-operated (n = 5), and crushing injury on nerve bundles from MPG (n = 10) groups at each of one, two, and four weeks after injury. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3794738&req=5

f1-ijms-14-17511: Bladder weights in the control, sham-operated, and crushing injury on nerve bundles from the MPG to the bladder groups at one, two, and four weeks after injury. Measurement of bladder weights was done in control (n = 5), sham-operated (n = 5), and crushing injury on nerve bundles from MPG (n = 10) groups at each of one, two, and four weeks after injury. Results are expressed as mean ± standard deviation of the mean. * p < 0.05 compared with the control group, ** p < 0.05 compared with the sham-operated group.
Mentions: At one week after injury, the bladder weight of crushing injury on nerve bundles from the MPG group (740.9 ± 31.6 mg) was significantly increased compared with the control and sham-operated groups (193.4 ± 31.7 and 232.8 ± 55.2 mg, respectively) (p < 0.05). The bladder weight of crushing injury on nerve bundles from the MPG group (738.2 ± 36.2 mg) was significantly increased compared with the control and sham-operated groups (183.2 ± 42.5 and 193.4 ± 35.2 mg, respectively) at two weeks after injury (p < 0.05). Similar with one and two weeks after injury, the bladder weight of crushing injury on nerve bundles from the MPG group (711.5 ± 60.4 mg) was significantly increased compared with the control and sham-operated groups (160.9 ± 44.8 and 197.8 ± 48.2 mg, respectively) (p < 0.05) (Figure 1).

Bottom Line: However, M3 muscarinic receptor expression was not significantly changed.The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury.From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Seoul St. Mary's Hospital, the Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, Korea. hygeia@catholic.ac.kr

ABSTRACT
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-α, and ROCK-β was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.

Show MeSH
Related in: MedlinePlus