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Molecular identification of Ancylostoma caninum isolated from cats in southern China based on complete ITS sequence.

Liu Y, Zheng G, Alsarakibi M, Zhang X, Hu W, Lu P, Lin L, Tan L, Luo Q, Li G - Biomed Res Int (2013)

Bottom Line: Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world.Comparative analysis of three complete ITS sequences obtained from cat-derived A. caninum showed the same length (738 bp) as that of dog-derived A. caninum.However, the sequence variation range was 98.6%-100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A. caninum isolates (AM850106, EU159416) in the same studied area.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT
Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence of A. caninum in cats in Guangzhou, southern China. Of the 102 fecal samples examined, the prevalence of A. caninum in cats was 95.1% and 83.3% using PCR-RFLP and microscopy, respectively. Among them, the prevalence of single hookworm infection with A. caninum was 54.90%, while mixed infections with both A. caninum and A. ceylanicum were 40.20%. Comparative analysis of three complete ITS sequences obtained from cat-derived A. caninum showed the same length (738 bp) as that of dog-derived A. caninum. However, the sequence variation range was 98.6%-100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A. caninum isolates (AM850106, EU159416) in the same studied area. The phylogenetic tree revealed A. caninum derived from both cats and dogs in single cluster. Results suggest that cats could be the main host of A. caninum in China, which may cause cross-infection between dogs and cats in the same area.

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Related in: MedlinePlus

PCR amplicons digested by restriction endonucleases Taq I. Lanes 1–53: random samples from 56 samples; P: PCR amplicon; M: DL-2000 DNA marker.
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fig2: PCR amplicons digested by restriction endonucleases Taq I. Lanes 1–53: random samples from 56 samples; P: PCR amplicon; M: DL-2000 DNA marker.

Mentions: The results showed that U. stenocephala was absent in the 97 examined PCR samples, where there was no enzymatic digestion by restriction endonucleases BsuRI (not shown). Figure 1 shows the digestion results of the 97 PCR amplicons by restriction endonucleases EcoR II, in which 41 samples showed three bands, revealing that these samples were mixed infections with A. ceylanicum and one undetermined hookworm (A. caninum or other), while 56 samples showed one band, revealing that these samples were single infection with one undetermined hookworm (A. caninum or other). Fifty three samples from the 56 single infection samples were randomly chosen for the Taq I digestion; the results show that all these samples were infected with only one species (Figure 2). Thereafter, 11 positive samples (6/56 and 5/41) were successfully sequenced; the phylogenetic tree based on those sequences with 15 reference sequences (Figure 3) revealed that the 56 positive samples were infected with A. caninum, while the 41 samples were infected with both A. ceylanicum and A. caninum, without any A. tubaeforme infection in our study. Thus, the overall prevalence of A. caninum infection was 95.1%, with a prevalence of 54.9% single infection and 40.2% mixed infections with A. ceylanicum.


Molecular identification of Ancylostoma caninum isolated from cats in southern China based on complete ITS sequence.

Liu Y, Zheng G, Alsarakibi M, Zhang X, Hu W, Lu P, Lin L, Tan L, Luo Q, Li G - Biomed Res Int (2013)

PCR amplicons digested by restriction endonucleases Taq I. Lanes 1–53: random samples from 56 samples; P: PCR amplicon; M: DL-2000 DNA marker.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794661&req=5

fig2: PCR amplicons digested by restriction endonucleases Taq I. Lanes 1–53: random samples from 56 samples; P: PCR amplicon; M: DL-2000 DNA marker.
Mentions: The results showed that U. stenocephala was absent in the 97 examined PCR samples, where there was no enzymatic digestion by restriction endonucleases BsuRI (not shown). Figure 1 shows the digestion results of the 97 PCR amplicons by restriction endonucleases EcoR II, in which 41 samples showed three bands, revealing that these samples were mixed infections with A. ceylanicum and one undetermined hookworm (A. caninum or other), while 56 samples showed one band, revealing that these samples were single infection with one undetermined hookworm (A. caninum or other). Fifty three samples from the 56 single infection samples were randomly chosen for the Taq I digestion; the results show that all these samples were infected with only one species (Figure 2). Thereafter, 11 positive samples (6/56 and 5/41) were successfully sequenced; the phylogenetic tree based on those sequences with 15 reference sequences (Figure 3) revealed that the 56 positive samples were infected with A. caninum, while the 41 samples were infected with both A. ceylanicum and A. caninum, without any A. tubaeforme infection in our study. Thus, the overall prevalence of A. caninum infection was 95.1%, with a prevalence of 54.9% single infection and 40.2% mixed infections with A. ceylanicum.

Bottom Line: Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world.Comparative analysis of three complete ITS sequences obtained from cat-derived A. caninum showed the same length (738 bp) as that of dog-derived A. caninum.However, the sequence variation range was 98.6%-100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A. caninum isolates (AM850106, EU159416) in the same studied area.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT
Ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. A highly sensitive and species-specific PCR-RFLP technique was utilised to detect the prevalence of A. caninum in cats in Guangzhou, southern China. Of the 102 fecal samples examined, the prevalence of A. caninum in cats was 95.1% and 83.3% using PCR-RFLP and microscopy, respectively. Among them, the prevalence of single hookworm infection with A. caninum was 54.90%, while mixed infections with both A. caninum and A. ceylanicum were 40.20%. Comparative analysis of three complete ITS sequences obtained from cat-derived A. caninum showed the same length (738 bp) as that of dog-derived A. caninum. However, the sequence variation range was 98.6%-100%, where only one cat isolate (M63) showed 100% sequence similarity in comparison with two dog-derived A. caninum isolates (AM850106, EU159416) in the same studied area. The phylogenetic tree revealed A. caninum derived from both cats and dogs in single cluster. Results suggest that cats could be the main host of A. caninum in China, which may cause cross-infection between dogs and cats in the same area.

Show MeSH
Related in: MedlinePlus