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New guar biopolymer silver nanocomposites for wound healing applications.

Ghosh Auddy R, Abdullah MF, Das S, Roy P, Datta S, Mukherjee A - Biomed Res Int (2013)

Bottom Line: The total protein, DNA, and hydroxyproline contents of the wound tissues were also significantly higher in the treated group as compared with the silver alginate cream (P < 0.05).Silver nanoparticles exerted positive effects because of their antimicrobial properties.The derivatized guar gum matrix additionally provided a hydrated surface necessary for cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, West Bengal 700009, India.

ABSTRACT
Wound healing is an innate physiological response that helps restore cellular and anatomic continuity of a tissue. Selective biodegradable and biocompatible polymer materials have provided useful scaffolds for wound healing and assisted cellular messaging. In the present study, guar gum, a polymeric galactomannan, was intrinsically modified to a new cationic biopolymer guar gum alkylamine (GGAA) for wound healing applications. Biologically synthesized silver nanoparticles (Agnp) were further impregnated in GGAA for extended evaluations in punch wound models in rodents. SEM studies showed silver nanoparticles well dispersed in the new guar matrix with a particle size of ~18 nm. In wound healing experiments, faster healing and improved cosmetic appearance were observed in the new nanobiomaterial treated group compared to commercially available silver alginate cream. The total protein, DNA, and hydroxyproline contents of the wound tissues were also significantly higher in the treated group as compared with the silver alginate cream (P < 0.05). Silver nanoparticles exerted positive effects because of their antimicrobial properties. The nanobiomaterial was observed to promote wound closure by inducing proliferation and migration of the keratinocytes at the wound site. The derivatized guar gum matrix additionally provided a hydrated surface necessary for cell proliferation.

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X-ray diffraction pattern of NAg-GGAA.
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Related In: Results  -  Collection


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fig2: X-ray diffraction pattern of NAg-GGAA.

Mentions: The XRD patterns of silver nanoparticles indicated a crystalline nature (Figure 2). Silver oxide peak at 2θ 38 degrees was negligible indicating purity of nanoparticles even after storage. This is likely due to GGAA protective capping of silver nanoparticles. Hundred-percent crystalline peak was observed at 2θ, 72 degree and corresponded with a near-spherical structure.


New guar biopolymer silver nanocomposites for wound healing applications.

Ghosh Auddy R, Abdullah MF, Das S, Roy P, Datta S, Mukherjee A - Biomed Res Int (2013)

X-ray diffraction pattern of NAg-GGAA.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794655&req=5

fig2: X-ray diffraction pattern of NAg-GGAA.
Mentions: The XRD patterns of silver nanoparticles indicated a crystalline nature (Figure 2). Silver oxide peak at 2θ 38 degrees was negligible indicating purity of nanoparticles even after storage. This is likely due to GGAA protective capping of silver nanoparticles. Hundred-percent crystalline peak was observed at 2θ, 72 degree and corresponded with a near-spherical structure.

Bottom Line: The total protein, DNA, and hydroxyproline contents of the wound tissues were also significantly higher in the treated group as compared with the silver alginate cream (P < 0.05).Silver nanoparticles exerted positive effects because of their antimicrobial properties.The derivatized guar gum matrix additionally provided a hydrated surface necessary for cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Technology, University of Calcutta, 92 A.P.C. Road, Kolkata, West Bengal 700009, India.

ABSTRACT
Wound healing is an innate physiological response that helps restore cellular and anatomic continuity of a tissue. Selective biodegradable and biocompatible polymer materials have provided useful scaffolds for wound healing and assisted cellular messaging. In the present study, guar gum, a polymeric galactomannan, was intrinsically modified to a new cationic biopolymer guar gum alkylamine (GGAA) for wound healing applications. Biologically synthesized silver nanoparticles (Agnp) were further impregnated in GGAA for extended evaluations in punch wound models in rodents. SEM studies showed silver nanoparticles well dispersed in the new guar matrix with a particle size of ~18 nm. In wound healing experiments, faster healing and improved cosmetic appearance were observed in the new nanobiomaterial treated group compared to commercially available silver alginate cream. The total protein, DNA, and hydroxyproline contents of the wound tissues were also significantly higher in the treated group as compared with the silver alginate cream (P < 0.05). Silver nanoparticles exerted positive effects because of their antimicrobial properties. The nanobiomaterial was observed to promote wound closure by inducing proliferation and migration of the keratinocytes at the wound site. The derivatized guar gum matrix additionally provided a hydrated surface necessary for cell proliferation.

Show MeSH