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Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.

Kuete V, Fankam AG, Wiench B, Efferth T - Evid Based Complement Alternat Med (2013)

Bottom Line: Results.Conclusion.The three active plants may be a source for the development of new anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.

No MeSH data available.


Related in: MedlinePlus

Effect of plant extracts and vinblastine (VIN) on the MMP of CCRF-CEM cells after 24 h of treatment. Data of control and vinblastine under similar experimental conditions were previously reported [33]. Samples were tested at their 1/4 × IC50 (1), 1/2 × IC50 (2), IC50 (3), and 2 × IC50 (4) values. The IC50 values are 0.20 μM for VIN, 10.57 μg/mL (Gladiolus quartinianus whole plant, GQW), 9.28 μg/mL (Vepris soyauxii leaves, VSL), and 17.32 μg/mL (Anonidium mannii leaves, AML).
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fig3: Effect of plant extracts and vinblastine (VIN) on the MMP of CCRF-CEM cells after 24 h of treatment. Data of control and vinblastine under similar experimental conditions were previously reported [33]. Samples were tested at their 1/4 × IC50 (1), 1/2 × IC50 (2), IC50 (3), and 2 × IC50 (4) values. The IC50 values are 0.20 μM for VIN, 10.57 μg/mL (Gladiolus quartinianus whole plant, GQW), 9.28 μg/mL (Vepris soyauxii leaves, VSL), and 17.32 μg/mL (Anonidium mannii leaves, AML).

Mentions: We assessed the effect of the GQW, VSL, and AML extracts on MMP in CCRF-CEM cells. As shown in Figure 3, percentage alterations of 13.5%, 28.9%, and 32.3% were induced by GQW, VSL, and AML extracts, respectively, after 24 h of treatment with twofold IC50. The MMP value for untreated cells was 4.81%. Under similar experimental conditions, these values were lower than that of the reference compound, vinblastine which yielded 48.6% as previously reported [33].


Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.

Kuete V, Fankam AG, Wiench B, Efferth T - Evid Based Complement Alternat Med (2013)

Effect of plant extracts and vinblastine (VIN) on the MMP of CCRF-CEM cells after 24 h of treatment. Data of control and vinblastine under similar experimental conditions were previously reported [33]. Samples were tested at their 1/4 × IC50 (1), 1/2 × IC50 (2), IC50 (3), and 2 × IC50 (4) values. The IC50 values are 0.20 μM for VIN, 10.57 μg/mL (Gladiolus quartinianus whole plant, GQW), 9.28 μg/mL (Vepris soyauxii leaves, VSL), and 17.32 μg/mL (Anonidium mannii leaves, AML).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794640&req=5

fig3: Effect of plant extracts and vinblastine (VIN) on the MMP of CCRF-CEM cells after 24 h of treatment. Data of control and vinblastine under similar experimental conditions were previously reported [33]. Samples were tested at their 1/4 × IC50 (1), 1/2 × IC50 (2), IC50 (3), and 2 × IC50 (4) values. The IC50 values are 0.20 μM for VIN, 10.57 μg/mL (Gladiolus quartinianus whole plant, GQW), 9.28 μg/mL (Vepris soyauxii leaves, VSL), and 17.32 μg/mL (Anonidium mannii leaves, AML).
Mentions: We assessed the effect of the GQW, VSL, and AML extracts on MMP in CCRF-CEM cells. As shown in Figure 3, percentage alterations of 13.5%, 28.9%, and 32.3% were induced by GQW, VSL, and AML extracts, respectively, after 24 h of treatment with twofold IC50. The MMP value for untreated cells was 4.81%. Under similar experimental conditions, these values were lower than that of the reference compound, vinblastine which yielded 48.6% as previously reported [33].

Bottom Line: Results.Conclusion.The three active plants may be a source for the development of new anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.

No MeSH data available.


Related in: MedlinePlus