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Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.

Kuete V, Fankam AG, Wiench B, Efferth T - Evid Based Complement Alternat Med (2013)

Bottom Line: Results.Conclusion.The three active plants may be a source for the development of new anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.

No MeSH data available.


Related in: MedlinePlus

Cell-cycle distribution of CCRF-CEM cells treated with plant extractsordoxorubicin at their corresponding IC50 values for 72 h. Data of control and doxorubicin obtained under similar experimental conditions were previously reported [33]. Flow cytometry histograms are available as supportive information (Figure S1).
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fig2: Cell-cycle distribution of CCRF-CEM cells treated with plant extractsordoxorubicin at their corresponding IC50 values for 72 h. Data of control and doxorubicin obtained under similar experimental conditions were previously reported [33]. Flow cytometry histograms are available as supportive information (Figure S1).

Mentions: The cell-cycle distribution and induction of apoptosis of CCRF-CEM cells upon treatment with GQW, VSL AML, are depicted in Figure 2. Upon 72 h treatment, the GQWextract induced cell cycle arrest between G0/G1 and S phases whilst VSL and AMLextracts induced G0/G1 arrest. The three extracts led to a time-dependent increase of sub-G0/G1 cells, indicating induction of apoptosis. CCRF-CEM cells treated with concentrations equivalent to the IC50 value of each studied extracts progressively underwent apoptosis, with percentages in sub-G0/G1 phase ranging from 11.2% (24 h) to 44.3% (72 h) for GQW, from 19.7% (24 h) to 53.2% (72 h) for VSL, and from 22.7% (24 h) to 76.2% (72 h) for AML. The values of the sub-G0/G1 phase recorded with AMLwere higher than those obtained with nontreated cells (range from 3.82% (24 h) to 9.37% (72 h)), but were comparable to those obtained for the control drug, doxorubicin (range from 59.4% (24 h) to 71.9% (72 h)) (see Supplementary Material available online at http://dx.doi.org/10.1155/2013/285903, Figure  S1).


Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.

Kuete V, Fankam AG, Wiench B, Efferth T - Evid Based Complement Alternat Med (2013)

Cell-cycle distribution of CCRF-CEM cells treated with plant extractsordoxorubicin at their corresponding IC50 values for 72 h. Data of control and doxorubicin obtained under similar experimental conditions were previously reported [33]. Flow cytometry histograms are available as supportive information (Figure S1).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794640&req=5

fig2: Cell-cycle distribution of CCRF-CEM cells treated with plant extractsordoxorubicin at their corresponding IC50 values for 72 h. Data of control and doxorubicin obtained under similar experimental conditions were previously reported [33]. Flow cytometry histograms are available as supportive information (Figure S1).
Mentions: The cell-cycle distribution and induction of apoptosis of CCRF-CEM cells upon treatment with GQW, VSL AML, are depicted in Figure 2. Upon 72 h treatment, the GQWextract induced cell cycle arrest between G0/G1 and S phases whilst VSL and AMLextracts induced G0/G1 arrest. The three extracts led to a time-dependent increase of sub-G0/G1 cells, indicating induction of apoptosis. CCRF-CEM cells treated with concentrations equivalent to the IC50 value of each studied extracts progressively underwent apoptosis, with percentages in sub-G0/G1 phase ranging from 11.2% (24 h) to 44.3% (72 h) for GQW, from 19.7% (24 h) to 53.2% (72 h) for VSL, and from 22.7% (24 h) to 76.2% (72 h) for AML. The values of the sub-G0/G1 phase recorded with AMLwere higher than those obtained with nontreated cells (range from 3.82% (24 h) to 9.37% (72 h)), but were comparable to those obtained for the control drug, doxorubicin (range from 59.4% (24 h) to 71.9% (72 h)) (see Supplementary Material available online at http://dx.doi.org/10.1155/2013/285903, Figure  S1).

Bottom Line: Results.Conclusion.The three active plants may be a source for the development of new anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.

No MeSH data available.


Related in: MedlinePlus