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Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.

Kuete V, Fankam AG, Wiench B, Efferth T - Evid Based Complement Alternat Med (2013)

Bottom Line: Results.Conclusion.The three active plants may be a source for the development of new anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.

No MeSH data available.


Related in: MedlinePlus

Growth (% of untreated control) of CCRF-CEM leukemia cells in the presence of plant extracts (40 μg/mL) or doxorubicin (10 μg/mL).
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fig1: Growth (% of untreated control) of CCRF-CEM leukemia cells in the presence of plant extracts (40 μg/mL) or doxorubicin (10 μg/mL).

Mentions: The growth inhibition of CCRF-CEM cells induced by 12 extracts belonging to six medicinal plants is depicted in Figure 1. The extracts from Gladiolus quartinianus (whole plant; GQW; 25.69%), Vepris soyauxii (leaves; VSL; 29.82%), and Anonidium mannii (leaves; AML; 31.58%) inhibited cell growth by more than 50% at 40 μg/mL.


Cytotoxicity and Modes of Action of the Methanol Extracts of Six Cameroonian Medicinal Plants against Multidrug-Resistant Tumor Cells.

Kuete V, Fankam AG, Wiench B, Efferth T - Evid Based Complement Alternat Med (2013)

Growth (% of untreated control) of CCRF-CEM leukemia cells in the presence of plant extracts (40 μg/mL) or doxorubicin (10 μg/mL).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794640&req=5

fig1: Growth (% of untreated control) of CCRF-CEM leukemia cells in the presence of plant extracts (40 μg/mL) or doxorubicin (10 μg/mL).
Mentions: The growth inhibition of CCRF-CEM cells induced by 12 extracts belonging to six medicinal plants is depicted in Figure 1. The extracts from Gladiolus quartinianus (whole plant; GQW; 25.69%), Vepris soyauxii (leaves; VSL; 29.82%), and Anonidium mannii (leaves; AML; 31.58%) inhibited cell growth by more than 50% at 40 μg/mL.

Bottom Line: Results.Conclusion.The three active plants may be a source for the development of new anticancer drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University of Mainz, Staudinger Weg 5, 55128 Mainz, Germany ; Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.

ABSTRACT
Introduction. The present study aims at evaluating the cytotoxicity of twelve parts from six Cameroonian medicinal plants on sensitive and drug-resistant cancer cell lines. We also studied the mode of action of the most active plants, Gladiolus quartinianus, Vepris soyauxii, and Anonidium mannii. Methods. The cytotoxicity of the extracts was determined using a resazurin assay. Flow cytometry was used for cell-cycle analysis and detection of apoptosis, analysis of mitochondrial membrane potential (MMP), and measurement of reactive oxygen species (ROS). Results. At 40 g/mL, three extracts showed a growth of CCRF-CEM leukemia cells by less than 50%. This includes the extracts from G. quartinianus (GQW; 25.69%), Vepris soyauxii leaves (VSL; 29.82%), and Anonidium mannii leaves (AML; 31.58%). The lowest IC50 values below 30  μ g/mL were obtained with GQW, AML and VSL against 7/9, 8/9, and 9/9 tested cancer cell lines, respectively. The lowest IC50 values for each plant were 4.09  μ g/mL, and 9.14  μ g/mL (against U87MG.ΔEGFR cells), respectively, for VSL and AML and 10.57  μ g/mL (against CCRF-CEM cells) for GQW. GQW induced cell cycle arrest between G0/G1 and S phases, whilst VSL and AML induced arrest in G0/G1. All three extracts induced apoptosis in CCRF-CEM cells by loss of MMP, whilst AML also enhanced production of ROS. Conclusion. The three active plants may be a source for the development of new anticancer drugs.

No MeSH data available.


Related in: MedlinePlus