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Skeletal muscle regeneration on protein-grafted and microchannel-patterned scaffold for hypopharyngeal tissue engineering.

Shen Z, Guo S, Ye D, Chen J, Kang C, Qiu S, Lu D, Li Q, Xu K, Lv J, Zhu Y - Biomed Res Int (2013)

Bottom Line: Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting.Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel.Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology of Lihuili Hospital, Ningbo University, Ningbo 315211, China.

ABSTRACT
In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.

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Related in: MedlinePlus

Cell morphology observed under SEM on (a) smooth PU-SF and (b) PU-SF with aligned microchannels. Cells were seeded at the density of 8 × 104 cells mL−1 and cultured for 10 days at 37°C in humidified air with 5% CO2 (the same conditions were followed for all cell culture).
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fig4: Cell morphology observed under SEM on (a) smooth PU-SF and (b) PU-SF with aligned microchannels. Cells were seeded at the density of 8 × 104 cells mL−1 and cultured for 10 days at 37°C in humidified air with 5% CO2 (the same conditions were followed for all cell culture).

Mentions: As the alignment of skeletal muscle cells is an important prerequisite for functional muscle tissue, a polymeric scaffold with unidirectional microchannels was designed to provide environmental cues to mimic the organization of myotubes in hypopharynx skeletal muscle. After 10 days of in vitro culture, the seeded myoblasts were found to be aligned parallel to the channels (Figure 4(b)). In contrast, cells cultured on smooth PU surface were found to be in random state (Figure 4(a)). Apart from being an important requirement for functional skeletal muscle tissue, such alignment may also potentially enhance myotube striation by restricting cell spreading to suppress myoblast proliferation and to promote cell fusion and skeletal muscle differentiation.


Skeletal muscle regeneration on protein-grafted and microchannel-patterned scaffold for hypopharyngeal tissue engineering.

Shen Z, Guo S, Ye D, Chen J, Kang C, Qiu S, Lu D, Li Q, Xu K, Lv J, Zhu Y - Biomed Res Int (2013)

Cell morphology observed under SEM on (a) smooth PU-SF and (b) PU-SF with aligned microchannels. Cells were seeded at the density of 8 × 104 cells mL−1 and cultured for 10 days at 37°C in humidified air with 5% CO2 (the same conditions were followed for all cell culture).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794632&req=5

fig4: Cell morphology observed under SEM on (a) smooth PU-SF and (b) PU-SF with aligned microchannels. Cells were seeded at the density of 8 × 104 cells mL−1 and cultured for 10 days at 37°C in humidified air with 5% CO2 (the same conditions were followed for all cell culture).
Mentions: As the alignment of skeletal muscle cells is an important prerequisite for functional muscle tissue, a polymeric scaffold with unidirectional microchannels was designed to provide environmental cues to mimic the organization of myotubes in hypopharynx skeletal muscle. After 10 days of in vitro culture, the seeded myoblasts were found to be aligned parallel to the channels (Figure 4(b)). In contrast, cells cultured on smooth PU surface were found to be in random state (Figure 4(a)). Apart from being an important requirement for functional skeletal muscle tissue, such alignment may also potentially enhance myotube striation by restricting cell spreading to suppress myoblast proliferation and to promote cell fusion and skeletal muscle differentiation.

Bottom Line: Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting.Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel.Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology of Lihuili Hospital, Ningbo University, Ningbo 315211, China.

ABSTRACT
In the field of tissue engineering, polymeric materials with high biocompatibility like polylactic acid and polyglycolic acid have been widely used for fabricating living constructs. For hypopharynx tissue engineering, skeletal muscle is one important functional part of the whole organ, which assembles the unidirectionally aligned myotubes. In this study, a polyurethane (PU) scaffold with microchannel patterns was used to provide aligning guidance for the seeded human myoblasts. Due to the low hydrophilicity of PU, the scaffold was grafted with silk fibroin (PU-SF) or gelatin (PU-Gel) to improve its cell adhesion properties. Scaffolds were observed to degrade slowly over time, and their mechanical properties and hydrophilicities were improved through the surface grafting. Also, the myoblasts seeded on PU-SF had the higher proliferative rate and better differentiation compared with those on the control or PU-Gel. Our results demonstrate that polyurethane scaffolds seeded with myoblasts hold promise to guide hypopharynx muscle regeneration.

Show MeSH
Related in: MedlinePlus