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Rhodiola rosea Impairs Acquisition and Expression of Conditioned Place Preference Induced by Cocaine.

Titomanlio F, Manzanedo C, Rodríguez-Arias M, Mattioli L, Perfumi M, Miñarro J, Aguilar MA - Evid Based Complement Alternat Med (2013)

Bottom Line: A novel approach to the treatment of adverse effects of drugs of abuse is one which makes use of natural products.Rhodiola did not induce motivational effects by itself but attenuated the acquisition and expression of cocaine-induced CPP.Moreover, it was found that RHO did not block reinstatement.

View Article: PubMed Central - PubMed

Affiliation: Pharmacognosy Unit, School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9, 62032 Camerino, Italy.

ABSTRACT
A novel approach to the treatment of adverse effects of drugs of abuse is one which makes use of natural products. The present study investigated the effect of Rhodiola rosea L. hydroalcoholic extract (RHO) on cocaine-induced hyperactivity and conditioned place preference (CPP) in mice. In a first experiment, mice received RHO (15, 20 or 25 mg/kg, IG), cocaine (25 mg/kg, i.p.) (COC), or a combination of both drugs (COC + RHO15, COC + RHO20, and COC + RHO25), and their locomotor activity was evaluated. In a second experiment, the effects of RHO on the acquisition, expression, and reinstatement of cocaine CPP (induced by drug priming or social defeat stress) were evaluated. RHO alone did not increase activity but potentiated the hyperactivity induced by cocaine. Rhodiola did not induce motivational effects by itself but attenuated the acquisition and expression of cocaine-induced CPP. Moreover, it was found that RHO did not block reinstatement. The results indicate that RHO is effective in reducing the rewarding properties of cocaine but is ineffective in preventing priming or stress-induced cocaine reinstatement. In light of these findings, the benefits of Rhodiola rosea L. as a treatment of cocaine addiction would seem to be limited.

No MeSH data available.


Related in: MedlinePlus

Effects of RHO on the priming- and stress-induced reinstatement of cocaine-induced CPP. Six groups of mice (n = 10–13 for group) were conditioned with cocaine (Coc 25 mg/kg, IP), underwent daily extinction sessions until the CPP was extinguished, and received the following treatments before the reinstatement test: vehicle, RHO 15, or 20 mg/kg 45 min before 12.5 mg/kg IP cocaine priming (Coc, Coc + RHO15, Coc + RHO20); vehicle, RHO 15, or 20 mg/kg 45 min before social defeat exposure (SD, SD + RHO15, SD + RHO20). Bars represent the time in seconds spent in the drug-paired compartment before conditioning sessions in the pre-C test (white bars), after conditioning sessions in the post-C test (black bars), in the last extinction session (light gray bars), and in the reinstatement test (dark gray bars). **P < 0.001, significant difference in the time spent in the drug-paired compartment in preconditioning versus post-conditioning or reinstatement test.
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fig4: Effects of RHO on the priming- and stress-induced reinstatement of cocaine-induced CPP. Six groups of mice (n = 10–13 for group) were conditioned with cocaine (Coc 25 mg/kg, IP), underwent daily extinction sessions until the CPP was extinguished, and received the following treatments before the reinstatement test: vehicle, RHO 15, or 20 mg/kg 45 min before 12.5 mg/kg IP cocaine priming (Coc, Coc + RHO15, Coc + RHO20); vehicle, RHO 15, or 20 mg/kg 45 min before social defeat exposure (SD, SD + RHO15, SD + RHO20). Bars represent the time in seconds spent in the drug-paired compartment before conditioning sessions in the pre-C test (white bars), after conditioning sessions in the post-C test (black bars), in the last extinction session (light gray bars), and in the reinstatement test (dark gray bars). **P < 0.001, significant difference in the time spent in the drug-paired compartment in preconditioning versus post-conditioning or reinstatement test.

Mentions: The effects of RHO on the priming- and social defeat-induced reinstatement of cocaine CPP are represented in Figure 4. The ANOVA revealed a significant effect of the variable days (F(3,59) = 26.101; P < 0.001). Bonferroni post hoc comparisons revealed that more time was spent in the drug-paired compartment during post-C and the reinstatement test than during pre-C or extinction. The variables treatment and interaction were not significant. These results show that RHO does not block the reinstatement of cocaine-CPP induced by priming or stress.


Rhodiola rosea Impairs Acquisition and Expression of Conditioned Place Preference Induced by Cocaine.

Titomanlio F, Manzanedo C, Rodríguez-Arias M, Mattioli L, Perfumi M, Miñarro J, Aguilar MA - Evid Based Complement Alternat Med (2013)

Effects of RHO on the priming- and stress-induced reinstatement of cocaine-induced CPP. Six groups of mice (n = 10–13 for group) were conditioned with cocaine (Coc 25 mg/kg, IP), underwent daily extinction sessions until the CPP was extinguished, and received the following treatments before the reinstatement test: vehicle, RHO 15, or 20 mg/kg 45 min before 12.5 mg/kg IP cocaine priming (Coc, Coc + RHO15, Coc + RHO20); vehicle, RHO 15, or 20 mg/kg 45 min before social defeat exposure (SD, SD + RHO15, SD + RHO20). Bars represent the time in seconds spent in the drug-paired compartment before conditioning sessions in the pre-C test (white bars), after conditioning sessions in the post-C test (black bars), in the last extinction session (light gray bars), and in the reinstatement test (dark gray bars). **P < 0.001, significant difference in the time spent in the drug-paired compartment in preconditioning versus post-conditioning or reinstatement test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794542&req=5

fig4: Effects of RHO on the priming- and stress-induced reinstatement of cocaine-induced CPP. Six groups of mice (n = 10–13 for group) were conditioned with cocaine (Coc 25 mg/kg, IP), underwent daily extinction sessions until the CPP was extinguished, and received the following treatments before the reinstatement test: vehicle, RHO 15, or 20 mg/kg 45 min before 12.5 mg/kg IP cocaine priming (Coc, Coc + RHO15, Coc + RHO20); vehicle, RHO 15, or 20 mg/kg 45 min before social defeat exposure (SD, SD + RHO15, SD + RHO20). Bars represent the time in seconds spent in the drug-paired compartment before conditioning sessions in the pre-C test (white bars), after conditioning sessions in the post-C test (black bars), in the last extinction session (light gray bars), and in the reinstatement test (dark gray bars). **P < 0.001, significant difference in the time spent in the drug-paired compartment in preconditioning versus post-conditioning or reinstatement test.
Mentions: The effects of RHO on the priming- and social defeat-induced reinstatement of cocaine CPP are represented in Figure 4. The ANOVA revealed a significant effect of the variable days (F(3,59) = 26.101; P < 0.001). Bonferroni post hoc comparisons revealed that more time was spent in the drug-paired compartment during post-C and the reinstatement test than during pre-C or extinction. The variables treatment and interaction were not significant. These results show that RHO does not block the reinstatement of cocaine-CPP induced by priming or stress.

Bottom Line: A novel approach to the treatment of adverse effects of drugs of abuse is one which makes use of natural products.Rhodiola did not induce motivational effects by itself but attenuated the acquisition and expression of cocaine-induced CPP.Moreover, it was found that RHO did not block reinstatement.

View Article: PubMed Central - PubMed

Affiliation: Pharmacognosy Unit, School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9, 62032 Camerino, Italy.

ABSTRACT
A novel approach to the treatment of adverse effects of drugs of abuse is one which makes use of natural products. The present study investigated the effect of Rhodiola rosea L. hydroalcoholic extract (RHO) on cocaine-induced hyperactivity and conditioned place preference (CPP) in mice. In a first experiment, mice received RHO (15, 20 or 25 mg/kg, IG), cocaine (25 mg/kg, i.p.) (COC), or a combination of both drugs (COC + RHO15, COC + RHO20, and COC + RHO25), and their locomotor activity was evaluated. In a second experiment, the effects of RHO on the acquisition, expression, and reinstatement of cocaine CPP (induced by drug priming or social defeat stress) were evaluated. RHO alone did not increase activity but potentiated the hyperactivity induced by cocaine. Rhodiola did not induce motivational effects by itself but attenuated the acquisition and expression of cocaine-induced CPP. Moreover, it was found that RHO did not block reinstatement. The results indicate that RHO is effective in reducing the rewarding properties of cocaine but is ineffective in preventing priming or stress-induced cocaine reinstatement. In light of these findings, the benefits of Rhodiola rosea L. as a treatment of cocaine addiction would seem to be limited.

No MeSH data available.


Related in: MedlinePlus