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16S rRNA PCR-Denaturing Gradient Gel Electrophoresis of Oral Lactobacillus casei Group and Their Phenotypic Appearances.

Piwat S, Teanpaisan R - ISRN Microbiol (2013)

Bottom Line: It was found that inhibitory effect of L. paracasei was stronger than L. casei and L. rhamnosus.The acid production of L. paracasei was lower than L. casei and L. rhamnosus.In conclusion, the technique has been proven to be able to differentiate between closely related species in L. casei group and thus provide reliable information of their phenotypic appearances.

View Article: PubMed Central - PubMed

Affiliation: Common Oral Diseases and Epidemiology Research Center, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand ; Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand.

ABSTRACT
This study aimed to develop a 16S rRNA PCR-denaturing gradient gel electrophoresis (DGGE) to identify the species level of Lactobacillus casei group and to investigate their characteristics of acid production and inhibitory effect. PCR-DGGE has been developed based on the 16S rRNA gene, and a set of HDA-1-GC and HDA-2, designed at V2-V3 region, and another set of CARP-1-GC and CARP-2, designed at V1 region, have been used. The bacterial strains included L. casei ATCC 393, L. paracasei CCUG 32212, L. rhamnosus ATCC 7469, L. zeae CCUG 35515, and 46 clinical strains of L. casei/paracasei/rhamnosus. Inhibitory effect against Streptococcus mutans and acid production were examined. Results revealed that each type species strain and identified clinical isolate showed its own unique DGGE pattern using CARP1-GC and CARP2 primers. HDA1-GC and HDA2 primers could distinguish the strains of L. paracasei from L. casei. It was found that inhibitory effect of L. paracasei was stronger than L. casei and L. rhamnosus. The acid production of L. paracasei was lower than L. casei and L. rhamnosus. In conclusion, the technique has been proven to be able to differentiate between closely related species in L. casei group and thus provide reliable information of their phenotypic appearances.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effects of clinical strains of L. casei/paracasei/rhamnosus against S. mutans ATCC 25175. Boxplot shows median, percentile (first and third percentile), and the minimum-maximum distribution of value.
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fig3: Inhibitory effects of clinical strains of L. casei/paracasei/rhamnosus against S. mutans ATCC 25175. Boxplot shows median, percentile (first and third percentile), and the minimum-maximum distribution of value.

Mentions: The ability for growth inhibition of L. casei group against S. mutans ATCC 25175 is shown in Figure 3. A statistically significant difference among the species was found (Kruskal-Wallis Test, P < 0.05) after L. paracasei strains were identified separately. L. paracasei strains had a significantly higher inhibitory effect than either L. casei or L. rhamnosus (Mann-Whitney U Test, P < 0.01).


16S rRNA PCR-Denaturing Gradient Gel Electrophoresis of Oral Lactobacillus casei Group and Their Phenotypic Appearances.

Piwat S, Teanpaisan R - ISRN Microbiol (2013)

Inhibitory effects of clinical strains of L. casei/paracasei/rhamnosus against S. mutans ATCC 25175. Boxplot shows median, percentile (first and third percentile), and the minimum-maximum distribution of value.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3794536&req=5

fig3: Inhibitory effects of clinical strains of L. casei/paracasei/rhamnosus against S. mutans ATCC 25175. Boxplot shows median, percentile (first and third percentile), and the minimum-maximum distribution of value.
Mentions: The ability for growth inhibition of L. casei group against S. mutans ATCC 25175 is shown in Figure 3. A statistically significant difference among the species was found (Kruskal-Wallis Test, P < 0.05) after L. paracasei strains were identified separately. L. paracasei strains had a significantly higher inhibitory effect than either L. casei or L. rhamnosus (Mann-Whitney U Test, P < 0.01).

Bottom Line: It was found that inhibitory effect of L. paracasei was stronger than L. casei and L. rhamnosus.The acid production of L. paracasei was lower than L. casei and L. rhamnosus.In conclusion, the technique has been proven to be able to differentiate between closely related species in L. casei group and thus provide reliable information of their phenotypic appearances.

View Article: PubMed Central - PubMed

Affiliation: Common Oral Diseases and Epidemiology Research Center, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand ; Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand.

ABSTRACT
This study aimed to develop a 16S rRNA PCR-denaturing gradient gel electrophoresis (DGGE) to identify the species level of Lactobacillus casei group and to investigate their characteristics of acid production and inhibitory effect. PCR-DGGE has been developed based on the 16S rRNA gene, and a set of HDA-1-GC and HDA-2, designed at V2-V3 region, and another set of CARP-1-GC and CARP-2, designed at V1 region, have been used. The bacterial strains included L. casei ATCC 393, L. paracasei CCUG 32212, L. rhamnosus ATCC 7469, L. zeae CCUG 35515, and 46 clinical strains of L. casei/paracasei/rhamnosus. Inhibitory effect against Streptococcus mutans and acid production were examined. Results revealed that each type species strain and identified clinical isolate showed its own unique DGGE pattern using CARP1-GC and CARP2 primers. HDA1-GC and HDA2 primers could distinguish the strains of L. paracasei from L. casei. It was found that inhibitory effect of L. paracasei was stronger than L. casei and L. rhamnosus. The acid production of L. paracasei was lower than L. casei and L. rhamnosus. In conclusion, the technique has been proven to be able to differentiate between closely related species in L. casei group and thus provide reliable information of their phenotypic appearances.

No MeSH data available.


Related in: MedlinePlus