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A Comparison between Extract Products of Magnolia officinalis on Memory Impairment and Amyloidogenesis in a Transgenic Mouse Model of Alzheimer's Disease.

Lee YJ, Choi DY, Han SB, Kim YH, Kim KH, Seong YH, Oh KW, Hong JT - Biomol Ther (Seoul) (2012)

Bottom Line: The components of Magnolia officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities.Oral pretreatment of two extract products of Magnolia officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented Aβ accumulation in the brain of Tg2576 mice.Therefore, our results showed that extract products of Magnolia officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating β-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Medical Research Center ; CBITRC ; Chungbuk National University, Cheongju 361-763.

ABSTRACT
The components of Magnolia officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities. These efficacies have been sold many products as nutritional supplement extracted from bark of Magnolia officinalis. Thus, to assess and compare neuroprotective effect in the nutritional supplement (Magnolia Extract(TM), Health Freedom Nutrition LLC, USA) and our ethanol extract of Magnolia officinalis (BioLand LTD, Korea), we investigated memorial improving and anti-Alzheimer's disease effects of extract products of Magnolia officinalis in a transgenic AD mice model. Oral pretreatment of two extract products of Magnolia officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented Aβ accumulation in the brain of Tg2576 mice. In addition, extract products of Magnolia officinalis also decreased expression of β-site APP cleaving enzyme 1 (BACE1), amyloid precursor protein (APP) and its product, C99. Although both two extract products of Magnolia officinalis could show preventive effect of memorial dysfunction and Aβ accumulation, our ethanol extract of Magnolia officinalis (BioLand LTD, Korea) could be more effective than Magnolia Extract(TM) (Health Freedom Nutrition LLC, USA). Therefore, our results showed that extract products of Magnolia officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating β-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.

No MeSH data available.


Related in: MedlinePlus

Memory improving effect of two ME products on probe test (A) and step-through test (B) in Tg2576 mice. The time spent in target zone in a probe test (A) conducted following the completion of training was recorded. Values are presented as mean ± S.E.M. from 10 mice. To perform passive avoidance test (B), the mice were given electric shock when entered dark room for training on learning day. After one day, of the learning day the retention time in illuminated compartment was recorded. Values are presented as mean ± S.E.M. from 10 mice. #Significant difference from non-treated Tg2576 mice (p<0.05). BL-ME: Ethanol extract of Magnolia officinalis from Bioland LTD HFN-ME: Extract product of Magnolia officinalis from Health Freedom Nutrition LLC.
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Figure 003: Memory improving effect of two ME products on probe test (A) and step-through test (B) in Tg2576 mice. The time spent in target zone in a probe test (A) conducted following the completion of training was recorded. Values are presented as mean ± S.E.M. from 10 mice. To perform passive avoidance test (B), the mice were given electric shock when entered dark room for training on learning day. After one day, of the learning day the retention time in illuminated compartment was recorded. Values are presented as mean ± S.E.M. from 10 mice. #Significant difference from non-treated Tg2576 mice (p<0.05). BL-ME: Ethanol extract of Magnolia officinalis from Bioland LTD HFN-ME: Extract product of Magnolia officinalis from Health Freedom Nutrition LLC.

Mentions: After the water maze test, we performed a probe test to analyze maintenance of memory. During the probe test, the time spent in the target quadrant on the BL-ME treated group (43.1 ± 8.9%, p=0.002) and the HFN-ME treated group (25.6± 8.2%, p=0.043) was significantly increased compared to the non-treated Tg2576 group (12.5 ± 10.4%) (Fig. 3A). Although all of ME products showed to ameliorate memorial dysfunction in memorial ability test using probe test, BL-ME treatment was also more effective than the HFN-ME treatment. We then evaluated learning and memory capacities by the passive avoidance test by step-through method. In the passive avoidance test, there was no significant difference on the learning trial. However, in test trial, step-through latency of the non-treated Tg2576 mice group (51.1 ± 36.6 sec) significantly increased to 114.3 ± 27.8 sec (p=0.005) by BL-ME treatment and 94.9± 31.2 sec (p=0.02) by HFN-ME treatment (Fig. 3B), and effects of two ME products did not show significant difference in step-through test.


A Comparison between Extract Products of Magnolia officinalis on Memory Impairment and Amyloidogenesis in a Transgenic Mouse Model of Alzheimer's Disease.

Lee YJ, Choi DY, Han SB, Kim YH, Kim KH, Seong YH, Oh KW, Hong JT - Biomol Ther (Seoul) (2012)

Memory improving effect of two ME products on probe test (A) and step-through test (B) in Tg2576 mice. The time spent in target zone in a probe test (A) conducted following the completion of training was recorded. Values are presented as mean ± S.E.M. from 10 mice. To perform passive avoidance test (B), the mice were given electric shock when entered dark room for training on learning day. After one day, of the learning day the retention time in illuminated compartment was recorded. Values are presented as mean ± S.E.M. from 10 mice. #Significant difference from non-treated Tg2576 mice (p<0.05). BL-ME: Ethanol extract of Magnolia officinalis from Bioland LTD HFN-ME: Extract product of Magnolia officinalis from Health Freedom Nutrition LLC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3794532&req=5

Figure 003: Memory improving effect of two ME products on probe test (A) and step-through test (B) in Tg2576 mice. The time spent in target zone in a probe test (A) conducted following the completion of training was recorded. Values are presented as mean ± S.E.M. from 10 mice. To perform passive avoidance test (B), the mice were given electric shock when entered dark room for training on learning day. After one day, of the learning day the retention time in illuminated compartment was recorded. Values are presented as mean ± S.E.M. from 10 mice. #Significant difference from non-treated Tg2576 mice (p<0.05). BL-ME: Ethanol extract of Magnolia officinalis from Bioland LTD HFN-ME: Extract product of Magnolia officinalis from Health Freedom Nutrition LLC.
Mentions: After the water maze test, we performed a probe test to analyze maintenance of memory. During the probe test, the time spent in the target quadrant on the BL-ME treated group (43.1 ± 8.9%, p=0.002) and the HFN-ME treated group (25.6± 8.2%, p=0.043) was significantly increased compared to the non-treated Tg2576 group (12.5 ± 10.4%) (Fig. 3A). Although all of ME products showed to ameliorate memorial dysfunction in memorial ability test using probe test, BL-ME treatment was also more effective than the HFN-ME treatment. We then evaluated learning and memory capacities by the passive avoidance test by step-through method. In the passive avoidance test, there was no significant difference on the learning trial. However, in test trial, step-through latency of the non-treated Tg2576 mice group (51.1 ± 36.6 sec) significantly increased to 114.3 ± 27.8 sec (p=0.005) by BL-ME treatment and 94.9± 31.2 sec (p=0.02) by HFN-ME treatment (Fig. 3B), and effects of two ME products did not show significant difference in step-through test.

Bottom Line: The components of Magnolia officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities.Oral pretreatment of two extract products of Magnolia officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented Aβ accumulation in the brain of Tg2576 mice.Therefore, our results showed that extract products of Magnolia officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating β-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Medical Research Center ; CBITRC ; Chungbuk National University, Cheongju 361-763.

ABSTRACT
The components of Magnolia officinalis have well known to act anti-inflammatory, anti-oxidative and neuroprotective activities. These efficacies have been sold many products as nutritional supplement extracted from bark of Magnolia officinalis. Thus, to assess and compare neuroprotective effect in the nutritional supplement (Magnolia Extract(TM), Health Freedom Nutrition LLC, USA) and our ethanol extract of Magnolia officinalis (BioLand LTD, Korea), we investigated memorial improving and anti-Alzheimer's disease effects of extract products of Magnolia officinalis in a transgenic AD mice model. Oral pretreatment of two extract products of Magnolia officinalis (10 mg/kg/day in 0.05% ethanol) into drinking water for 3 months ameliorated memorial dysfunction and prevented Aβ accumulation in the brain of Tg2576 mice. In addition, extract products of Magnolia officinalis also decreased expression of β-site APP cleaving enzyme 1 (BACE1), amyloid precursor protein (APP) and its product, C99. Although both two extract products of Magnolia officinalis could show preventive effect of memorial dysfunction and Aβ accumulation, our ethanol extract of Magnolia officinalis (BioLand LTD, Korea) could be more effective than Magnolia Extract(TM) (Health Freedom Nutrition LLC, USA). Therefore, our results showed that extract products of Magnolia officinalis were effective for prevention and treatment of AD through memorial improving and anti-amyloidogenic effects via down-regulating β-secretase activity, and neuroprotective efficacy of Magnolia extracts could be differed by cultivating area and manufacturing methods.

No MeSH data available.


Related in: MedlinePlus