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Hesperidin Induces Apoptosis by Inhibiting Sp1 and Its Regulatory Protein in MSTO-211H Cells.

Lee KA, Lee SH, Lee YJ, Baeg SM, Shim JH - Biomol Ther (Seoul) (2012)

Bottom Line: The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h.Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death.Hesperidin increased Sub-G1 population in MSTO-211H cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Medicine, Soonchunhyang University.

ABSTRACT
Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-G1 population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-xl in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma.

No MeSH data available.


Related in: MedlinePlus

The effect of hesperidin on apoptosis of hesperidin-treated MSTO-211H. Immunoblot detection of the Bid, Bcl-xL, Cleaved-Caspase-3, PARP, and Bax in whole cell lysates from various concentration of hesperidin-treated MSTO-211H cells for 48 h. β-actin was used to normalize the protein loading from each treatment.
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Figure 006: The effect of hesperidin on apoptosis of hesperidin-treated MSTO-211H. Immunoblot detection of the Bid, Bcl-xL, Cleaved-Caspase-3, PARP, and Bax in whole cell lysates from various concentration of hesperidin-treated MSTO-211H cells for 48 h. β-actin was used to normalize the protein loading from each treatment.

Mentions: In order to investigate how hesperidin regulated the expression of apoptotic marker proteins in MSTO-211H cells, we determined the level of several pro- and anti-apoptotic proteins in hesperidin-treated cells by immunoblotting after treatment with hesperidin for 48 h, the activation of PARP and Bid, the cleavage of caspase3, and the induction of Bax, which are the hallmarks of apoptotic activity as well as the down-regulation of Bcl-xl in MSTO-211H cells (Fig. 6).


Hesperidin Induces Apoptosis by Inhibiting Sp1 and Its Regulatory Protein in MSTO-211H Cells.

Lee KA, Lee SH, Lee YJ, Baeg SM, Shim JH - Biomol Ther (Seoul) (2012)

The effect of hesperidin on apoptosis of hesperidin-treated MSTO-211H. Immunoblot detection of the Bid, Bcl-xL, Cleaved-Caspase-3, PARP, and Bax in whole cell lysates from various concentration of hesperidin-treated MSTO-211H cells for 48 h. β-actin was used to normalize the protein loading from each treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3794523&req=5

Figure 006: The effect of hesperidin on apoptosis of hesperidin-treated MSTO-211H. Immunoblot detection of the Bid, Bcl-xL, Cleaved-Caspase-3, PARP, and Bax in whole cell lysates from various concentration of hesperidin-treated MSTO-211H cells for 48 h. β-actin was used to normalize the protein loading from each treatment.
Mentions: In order to investigate how hesperidin regulated the expression of apoptotic marker proteins in MSTO-211H cells, we determined the level of several pro- and anti-apoptotic proteins in hesperidin-treated cells by immunoblotting after treatment with hesperidin for 48 h, the activation of PARP and Bid, the cleavage of caspase3, and the induction of Bax, which are the hallmarks of apoptotic activity as well as the down-regulation of Bcl-xl in MSTO-211H cells (Fig. 6).

Bottom Line: The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h.Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death.Hesperidin increased Sub-G1 population in MSTO-211H cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Medicine, Soonchunhyang University.

ABSTRACT
Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-G1 population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-xl in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma.

No MeSH data available.


Related in: MedlinePlus