Limits...
Hesperidin Induces Apoptosis by Inhibiting Sp1 and Its Regulatory Protein in MSTO-211H Cells.

Lee KA, Lee SH, Lee YJ, Baeg SM, Shim JH - Biomol Ther (Seoul) (2012)

Bottom Line: The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h.Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death.Hesperidin increased Sub-G1 population in MSTO-211H cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Medicine, Soonchunhyang University.

ABSTRACT
Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-G1 population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-xl in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma.

No MeSH data available.


Related in: MedlinePlus

The apoptotic effect induced by hesperidin in MSTO-211H cells. Cells were incubated with hesperidin (40 80 and 160 μM) treated and untreated (DMSO) of MSTO-211H cells for 48 h. The cells were harvested and prepared for DAPI staining and PI staining as described under Materials and Methods. (A) Analysis of DNA fragmentation and nuclear condensation (white arrows) by fluorescence microscopy (magnification ×600) after treatment of hesperidin in MSTO-211H cells (B) DNA fragmentation and nuclear condensation were quantified and the results in triplicates are expressed as the mean ± SD. (C) Analysis of cell cycle by flow cytometry after hesperidin treatment of cells for 48 h. (D) Representative histograms of Sub-G1 population. The hesperidin-treated cells were compared with untreated cells, and the graphs are shown as the average of triplicated data from three independent experiments (*p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3794523&req=5

Figure 002: The apoptotic effect induced by hesperidin in MSTO-211H cells. Cells were incubated with hesperidin (40 80 and 160 μM) treated and untreated (DMSO) of MSTO-211H cells for 48 h. The cells were harvested and prepared for DAPI staining and PI staining as described under Materials and Methods. (A) Analysis of DNA fragmentation and nuclear condensation (white arrows) by fluorescence microscopy (magnification ×600) after treatment of hesperidin in MSTO-211H cells (B) DNA fragmentation and nuclear condensation were quantified and the results in triplicates are expressed as the mean ± SD. (C) Analysis of cell cycle by flow cytometry after hesperidin treatment of cells for 48 h. (D) Representative histograms of Sub-G1 population. The hesperidin-treated cells were compared with untreated cells, and the graphs are shown as the average of triplicated data from three independent experiments (*p<0.05).

Mentions: condensation and fragmentation by DAPI. As shown in Fig. 2A, hesperidin treatment of mesothelioma cells led to an increase in nuclear condensation and fragmentation compared with the control. We determined the effect of hesperidin on Sub-G1 population in mesothelioma cells by PI staining (Fig. 2C and D). It showed that the Sub-G1 phase increased from


Hesperidin Induces Apoptosis by Inhibiting Sp1 and Its Regulatory Protein in MSTO-211H Cells.

Lee KA, Lee SH, Lee YJ, Baeg SM, Shim JH - Biomol Ther (Seoul) (2012)

The apoptotic effect induced by hesperidin in MSTO-211H cells. Cells were incubated with hesperidin (40 80 and 160 μM) treated and untreated (DMSO) of MSTO-211H cells for 48 h. The cells were harvested and prepared for DAPI staining and PI staining as described under Materials and Methods. (A) Analysis of DNA fragmentation and nuclear condensation (white arrows) by fluorescence microscopy (magnification ×600) after treatment of hesperidin in MSTO-211H cells (B) DNA fragmentation and nuclear condensation were quantified and the results in triplicates are expressed as the mean ± SD. (C) Analysis of cell cycle by flow cytometry after hesperidin treatment of cells for 48 h. (D) Representative histograms of Sub-G1 population. The hesperidin-treated cells were compared with untreated cells, and the graphs are shown as the average of triplicated data from three independent experiments (*p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3794523&req=5

Figure 002: The apoptotic effect induced by hesperidin in MSTO-211H cells. Cells were incubated with hesperidin (40 80 and 160 μM) treated and untreated (DMSO) of MSTO-211H cells for 48 h. The cells were harvested and prepared for DAPI staining and PI staining as described under Materials and Methods. (A) Analysis of DNA fragmentation and nuclear condensation (white arrows) by fluorescence microscopy (magnification ×600) after treatment of hesperidin in MSTO-211H cells (B) DNA fragmentation and nuclear condensation were quantified and the results in triplicates are expressed as the mean ± SD. (C) Analysis of cell cycle by flow cytometry after hesperidin treatment of cells for 48 h. (D) Representative histograms of Sub-G1 population. The hesperidin-treated cells were compared with untreated cells, and the graphs are shown as the average of triplicated data from three independent experiments (*p<0.05).
Mentions: condensation and fragmentation by DAPI. As shown in Fig. 2A, hesperidin treatment of mesothelioma cells led to an increase in nuclear condensation and fragmentation compared with the control. We determined the effect of hesperidin on Sub-G1 population in mesothelioma cells by PI staining (Fig. 2C and D). It showed that the Sub-G1 phase increased from

Bottom Line: The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h.Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death.Hesperidin increased Sub-G1 population in MSTO-211H cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, College of Medicine, Soonchunhyang University.

ABSTRACT
Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The IC50 value of hesperidin was determined to be 152.3 μM in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 μM) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-G1 population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-xl in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma.

No MeSH data available.


Related in: MedlinePlus